Human CD28H Full-length Gene in Lentivector, Endotoxin-free DNA

Product ID: pLTC-CD28H (SKU#: LTP1837)

Price:

$796.00

Description

CD28H (CD28 homolog), also known as TMIGD2 (transmembrane and immunoglobulin domain-containing protein 2) and IGPR1 (immunoglobulin and proline-rich receptor 1), is a single-pass type I transmembrane glycoprotein. CD28H contains 1 Ig (immunoglobulin)-like domain in the extracellular region and 1 cytoplasmic proline-rich domain. CD28H is widely expressed, mainly by epithelial and endothelial cells. It is also expressed in lymphoid organs, with highest levels in thymus, spleen, peripheral blood lymphocytes and liver. In the thymus, it is expressed in CD4 and CD8 single- and double-positive cells, but not in immature CD4 and CD8 double-negative cells. In peripheral blood mononuclear cells, CD28H is highly expressed on natural killer (NK) cells and CD3+ T-cells but not on B-cells. CD28H may form homophilic interactions that could regulate cell-cell interaction, cell migration, and angiogenesis. The B7 family member B7-H7 (B7 homolog 7), also know n as HHLA2 (human endogenous retrovirus-H long terminal repeat-associating protein 2), was identified as a specific ligand for CD28H. Through interaction with B7-H7, CD28H may co-stimulate T-cells in the context of TCR-mediated activation. The B7-H7/CD28H interaction selectively co-stimulates human T-cell growth and cytokine production via an AKT-dependent signalling cascade.

Product Details

Gene Symbol: CD28H; TMIGD2; IGPR1; IGPR-1;UNQ3059/PRO9879

NCBI Gene ID: 126259

Uniprot Entry: Q96BF3

Construct Details: Full length human CD28H/TMIGD2 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

Gene Insert Sequence:

ATGGGGTCCCCGGGCATGGTGCTGGGCCTCCTGGTGCAGATCTGGGCCCTGCAAGAAGCCTCAAGCCTGAGCGTGCAGCA

GGGGCCCAACTTGCTGCAGGTGAGGCAGGGCAGTCAGGCGACCCTGGTCTGCCAGGTGGACCAGGCCACAGCCTGGGAAC

GGCTCCGTGTTAAGTGGACAAAGGATGGGGCCATCCTGTGTCAACCGTACATCACCAACGGCAGCCTCAGCCTGGGGGTC

TGCGGGCCCCAGGGACGGCTCTCCTGGCAGGCACCCAGCCATCTCACCCTGCAGCTGGACCCTGTGAGCCTCAACCACAG

CGGGGCGTACGTGTGCTGGGCGGCCGTAGAGATTCCTGAGTTGGAGGAGGCTGAGGGCAACATAACAAGGCTCTTTGTGG

ACCCAGATGACCCCACACAGAACAGAAACCGGATCGCAAGCTTCCCAGGATTCCTCTTCGTGCTGCTGGGGGTGGGAAGC

ATGGGTGTGGCTGCGATCGTGTGGGGTGCCTGGTTCTGGGGCCGCCGCAGCTGCCAGCAAAGGGACTCAGGTAACAGCCC

AGGAAATGCATTCTACAGCAACGTCCTATACCGGCCCCGGGGGGCCCCAAAGAAGAGTGAGGACTGCTCTGGAGAGGGGA

AGGACCAGAGGGGCCAGAGCATTTATTCAACCTCCTTCCCGCAACCGGCCCCCCGCCAGCCGCACCTGGCGTCAAGACCC

TGCCCCAGCCCGAGACCCTGCCCCAGCCCCAGGCCCGGCCACCCCGTCTCTATGGTCAGGGTCTCTCCTAGACCAAGCCC

CACCCAGCAGCCGAGGCCAAAAGGGTTCCCCAAAGTGGGAGAGGAGTGA

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

Storage

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

References