CD28H (CD28 homolog), also known as TMIGD2 (transmembrane and immunoglobulin domain-containing protein 2) and IGPR1 (immunoglobulin and proline-rich receptor 1), is a single-pass type I transmembrane glycoprotein. CD28H contains 1 Ig (immunoglobulin)-like domain in the extracellular region and 1 cytoplasmic proline-rich domain.  CD28H is widely expressed, mainly by epithelial and endothelial cells.  It is also expressed in lymphoid organs, with highest levels in thymus, spleen, peripheral blood lymphocytes and liver. In the thymus, it is expressed in CD4 and CD8 single- and double-positive cells, but not in immature CD4 and CD8 double-negative cells. In peripheral blood mononuclear cells, CD28H is highly expressed on natural killer (NK) cells and CD3+ T-cells but not on B-cells. CD28H may form homophilic interactions that could regulate cell-cell interaction, cell migration, and angiogenesis. The B7 family member B7-H7 (B7 homolog 7), also know n as HHLA2 (human endogenous retrovirus-H long terminal repeat-associating protein 2), was identified as a specific ligand for CD28H. Through interaction with B7-H7, CD28H may co-stimulate T-cells in the context of TCR-mediated activation. The B7-H7/CD28H interaction selectively co-stimulates human T-cell growth and cytokine production via an AKT-dependent signalling cascade.

CD28H (CD28 homolog), also known as TMIGD2 (transmembrane and immunoglobulin domain-containing protein 2) and IGPR1 (immunoglobulin and proline-rich receptor 1), is a single-pass type I transmembrane glycoprotein. CD28H contains 1 Ig (immunoglobulin)-like domain in the extracellular region and 1 cytoplasmic proline-rich domain.  CD28H is widely expressed, mainly by epithelial and endothelial cells.  It is also expressed in lymphoid organs, with highest levels in thymus, spleen, peripheral blood lymphocytes and liver. In the thymus, it is expressed in CD4 and CD8 single- and double-positive cells, but not in immature CD4 and CD8 double-negative cells. In peripheral blood mononuclear cells, CD28H is highly expressed on natural killer (NK) cells and CD3+ T-cells but not on B-cells. CD28H may form homophilic interactions that could regulate cell-cell interaction, cell migration, and angiogenesis. The B7 family member B7-H7 (B7 homolog 7), also know n as HHLA2 (human endogenous retrovirus-H long terminal repeat-associating protein 2), was identified as a specific ligand for CD28H. Through interaction with B7-H7, CD28H may co-stimulate T-cells in the context of TCR-mediated activation. The B7-H7/CD28H interaction selectively co-stimulates human T-cell growth and cytokine production via an AKT-dependent signalling cascade.

 

Product Details

 

Gene Symbol: CD28H; TMIGD2; IGPR1; IGPR-1;UNQ3059/PRO9879

 

NCBI Gene ID: 126259

 

Uniprot Entry: Q96BF3

 

Construct Details: The recombinant human CD28H-Fc fusion is expressed as a 366-amino acid protein consisting of Leu23 - Gly150 region of CD28H (UniProt accession #Q96BF3) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition.

 

Source:  Human cells stably expressing CD28H-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

LSVQQGPNLL QVRQGSQATL VCQVDQATAW ERLRVKWTKD GAILCQPYIT NGSLSLGVCG 

        70         80         90        100        110        120 

PQGRLSWQAP SHLTLQLDPV SLNHSGAYVC WAAVEIPELE EAEGNITRLF VDPDDPTQNR 

       130        140        150        160        170        180 

NRIASFPGST TENLYFQGST GTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC 

       190        200        210        220        230        240 

VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC 

       250        260        270        280        290        300 

KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSREEMTKN QVSLTCLVKG FYPSDIAVEW 

       310        320        330        340        350        360 

ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL 

 

SLSPGK 

M.W.: Calculated molecular mass 40.7 kDa; estimated by SDS-PAGE under reducing condition 55-60 kDa probably due to glycosylation

 

Calculated PI: 6.17

 

Calculated Extinction Coefficients (M-1 cm-1, at 280nm):  62505

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier and preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to its B7 family ligand, B7-H7/HHLA2 (SKU#: FCL1038) and iInhibit B7-H7-mediated signaling activity including IL-2 secretion in  human T cells stimulated with anti-CD3 antibody.  

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

  

References

 

1. Mol. Biol. Cell 23:1646 (2012).

2. Nat. Commun. 4:2043-2043 (2013)

3. Proc. Natl. Acad. Sci. 110:9879 (2013).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL1824B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.