G&P Biosciences offers stable cell line establishment and customization services utilizing our high throughput gene expression platform. Our platform provides a rapid and cost-effective solution for generating custom stable cell lines, which can be used for cellular function study, drug screening, protein production, target validation, and many other research purposes. Our services are focused on optimizing the parameters specific to your cell lines and delivering stable cell lines according to your requirements.

Custom Cell Line Generation

(Service Code: CS-001)

We can engineer essentially any cell line to stably express your gene of interest, produce your protein of interest, or generate stable shRNA knocking down a specific gene. We bring years of cell culture experience and expertise for this service, including DNA transfection, stable cell selection, colony pickup, clonal expansion, functional screening, and expression validation by various bioassays. We have the experience with more than 60 cancer cell lines and highly efficient gene delivery methods.  We have the expertise to generate stable cell lines for many research and development applications:

• Functional assays in the fields such as GPCR, receptor, transporter & kinase
• Reporter expressing stable cell lines, such as luciferase, GFP, RFP, HRP, lacZ
• Protein & antibody production stable cell lines, such as CHO, 293, NS0, PerC6
• Stable gene knockdown (shRNA) cell lines

Bundled Service Package

One-Stop Shop Solution: Gene-To-Cell-line

(Service Code: CS-G2C)

We offer bundled stable cell line generation service, “Gene-to-Cell-line”, a one-stop shop solution with standardized workflow and reduced turnaround time. Bundle discount is included when you order the target gene expression construct and custom made stable cell lines from G&P Biosciences at the same time. We can synthesize and subcloneyour gene of interest into a suitable expression vector with desired selection marker. We can introduce the DNA construct into the host cells and select the stable pools/clones that express your gene of interest at desirable levels. We can help you validate the genomic integration by genomic PCR and the expression using ELISA, flow cytometry, Western blot, or other cell-based assay of your interest. We can also use established protocols to assess cell proliferation rates and expression stabilities for your custom-made cell lines.

To request this bundled service, simply provide us the DNA nucleotide or protein amino acid sequence for the target gene to be delivered, and indicate the host cell line to use and the desired number of independent stable clones required. Upon receipt of your request, our service team will contact you with the detailed construct design, stable cell generation & validation methods, pricing, and timeline information tailored to your needs.

Data Example

Two custom stable cell line projects are shown below as examples to demonstrate the speed and efficiency of our one-stop shop solution, “Gene-to-Cell-line” services:

Example #1

The client requested us to generate a stable mouse fibroblast cells expressing a gene of interest (GOI) fused with GFP.  In just 16 days, we were able to deliver the stable cells (pools) using our unique gene delivery and cell selection methods. Fluorescence microscopy confirmed GOI’s surface expression  (see the image below on the left) and subsequently the stable cells were employed by the client to develop a successful cell-based screening assay.

Example #2

The client requested to produce two independent stable clones of human cancer cells that constitutively and uniformly overexpress a cell surface receptor. In just 6 weeks, we were able to deliver the two stable clones after screening 12 independent single-cell lineages.  Flow cytometry analysis with PE-labeled monoclonal antibody (mAb-PE) against this surface receptor confirms the high and uniform surface expression in comparison with the parental cells (see the flow data below for one of the stable clones, #8). The client further used the the stable clones to establish a tumor animal model for in vivo efficacy studies.

Stable cell lines expressing a gene/protein of interest or a shRNA construct targeting a specific gene are important research tools for biological and pharmaceutical research. We provide custom research stable cell line services based on our unique expression vectors, gene delivery vehicles, cell selecting, screening and cloning technologies in a significantly reduced timeline. Our bundled cell line development services can accommodate a variety of stages and needs of your research and development process. With G&P Biosciences, you can count on our experience in the field of cell line establishment and customization to efficiently produce stable cell lines according to your requirements.

Additional Cell Services

Custom Cell Culture & Scale-up

(Service Code: CS-002)

We also provide custom cell culture services for cell-based assay development, bioproduction and other tissue culture-related needs.  We have a collections of pre-made DNA constructs, lentivirus, and common cell lines that permanently express different reporter genes such as luciferase and GFP for cell-based assays. Through our strategic partner, we can develop cell lines to produce therapeutic proteins and antibodies at industry production levels. We have the capacity to provide large-scale cell culture and conduct unbiased expression system assessment and optimization of existing cell lines of low productivities.

To discuss your project and receive a quote, please contact us by email: This email address is being protected from spambots. You need JavaScript enabled to view it.