G&P Biosciences offers custom gene synthesis, cloning and mutagenesis services utilizing our High Throughput Gene Cloning (HTGC) technology in combination with state-of-the-art gene synthesis, high fidelity PCR, and DNA assembly methods.  We perform seamless cloning of any DNA fragments, synthetic or PCR-amplified, into any vector including difficult retroviral vectors. We can synthesize and insert a codon-optimized gene into any expression vector for your transient or stable protein expression. We can modify the coding sequence using site-directed or systematic mutagenesis, and further optimize its expression through genetic & protein engineering. 

Custom Gene Synthesis

(Service Code: GS-001)

We offer de novo synthesis of any gene that is not on our list of pre-made, expression-ready clones. We can generate virtually any client-desired DNA sequences, cloned and 100% sequence-verified, ready for downstream applications.  We can assembly them in a standard cloning vector or the vector provided by the client at no additional charge.

Custom Gene Cloning

(Service Code: GS-002)

We provide custom cloning services at competitive rates and short turn-around time. We can subclone any DNA insert seamlessly into any vector of your choice (e.g., eukaryotic, prokaryotic, viral vectors). We have considerable experience in challenging cloning projects, such as those involvinglarge vector or insert sizes, and unstable DNA elements.

Custom Gene Mutagenesis

(Service Code: GS-003)

We can introduce any mutations, including single/multiple-point mutations, deletions, insertions, and substitutions, into your gene of interest. We can introduce mutations by site-directed or systematic mutagenesis, including client-defined modifications or random mutations at the desired region of an existing DNA template.

Bundled Service Package

One-Stop Shop Solution: Gene-To-Clone

(Service Code: GS-G2C)

We offer bundled gene synthesis and cloning service, “Gene-to-Clone”, a one-stop shop solution with standardized workflow and turnaround time. Bundle discount is included when you order the target gene to be synthesized and cloned by G&P Biosciences at the same time.

To order, simply provide the DNA nucleotide or protein amino acid sequence for the target gene to be delivered, and indicate the vector to use. If the protein sequence entered, select the species for codon optimization, e.g., mammalian, bacterial, mouse, or human.  Upon receipt of your request, our service team will contact you with the optimized sequence, detailed pricing and timeline information tailored to your needs.

Data Example

The experimental data from our three different bundled gene synthesis and cloning projects (project A, B, & C) are shown below as an example to illustrate the efficiency of our services.

The table below summarizes the feature and size for gene insert synthesized & vector cloned as well as the delivery time for each project.  For all three projects, ready-to-use, endotoxin-free DNA have been delivered to the clients within very short turnaround time after all DNA inserts have been verified by restriction digestion (see the gel picture below) and by DNA sequencing (data not shown).

The gel picture below shows the results from restriction digestion analysis of DNA samples prepared from multiple independent clones picked for each project (2 clones for projects A & B, 6 for project C).  As labeled by numbers (in kb) on the gel, all of these clones contain the inserts with correct sizes.

Using our cutting-edge High Throughput Gene Cloning and assembly methods, we are able to offer custom gene services at very high success rate and speed. We can synthesize and subclone any DNA inserts seamlessly into any vector with almost 100% success rates.  Ready-to-use, endotoxin-free DNA is delivered with each insert restriction digestion and 100% sequence verified.

Our custom gene services are also highly flexible to your specific needs with many fusions, tags, and selectable markers to choose from (see the lists below):

Additional Gene Services

Custom Vector Construction

(Service Code: GS-004)

Using our cloning and assembly strategies, we can help develop completely customized vectors quickly and reliably. We can design vectors from our collection of modular DNA fragments encoding tags, fusions, selectable markers, regulatory/functional elements or from client-provided elements. They can be customized to accommodate your needs and to achieve maximum benefits:

Average turnaround time is 3-4 weeks.

For details and pricing information, please contact us by email: This email address is being protected from spambots. You need JavaScript enabled to view it.

Custom Library Construction

(Service Code: GS-005)

From standard libraries to uncut, full-length, expression-ready libraries, we offer multiple options tailored for your research needs, beginning with a minimum of 100 µg of total RNA or 10 µg of poly(A+) RNA. We also provide custom construction of synthetic libraries containing random mutations at specific regions of gene of interest.

Average turnaround time is 10-12 weeks.

For details and pricing information, please contact us by email: This email address is being protected from spambots. You need JavaScript enabled to view it.