Human B7-H7 Full-length Gene in Lentivector, Endotoxin-free DNA

Product ID: pLTC-B7-H7 (SKU#: LTP1037)

Price:

$796.00

Description

B7-H7 (B7 homolog 7), also know n as HHLA2 (human endogenous retrovirus-H long terminal repeat-associating protein 2), is regarded as a new member of the B7 ligand family. B7-H7 contains 1 Ig-like (immunoglobulin-like) C1-type and 2 Ig-like V-type domains while other members are typically composed of 1 Ig-like V-type and 1 Ig-like C1-type domains. Interactions between members of the B7 ligand and CD28/CTLA4 receptor families can generate positive co-stimulation and negative co-inhibition in regulating T cell responses. B7-H7 is expressed at high levels in colon, kidney, testis, lung and pancreas, and at lower levels in small intestine, liver and skeletal muscle. In immune cells, B7-H7 is highly expressed in B-cells, dendritic cells, monocytes and macrophages, but not detected in T-cells. B7-H7 was reported to regulate cell-mediated immunity by binding to a putative receptor on T lymphocytes and inhibiting the proliferation of T cells. B7-H7 has also been referred to as B-H5 (B7 homolog 5), which was identified as a specific ligand for CD28H (CD28 homolog) or TMIGD2 (transmembrane and Ig domain-containing protein 2). B7-H5 is constitutively found in macrophages and could be induced on dendritic cells in response to inflammation. The B7-H5/CD28H interaction was shown to selectively co-stimulate human T-cell growth and cytokine production via an AKT-dependent signalling cascade.

Product Details

Gene Symbol: B7-H7; HHLA2; B7H7; B7-H5; B7H5

NCBI Gene ID: 11148

Uniprot Entry: Q9UM44

Construct Details: Full length human B7-H7/HHLA2 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

Gene Insert Size: 1245 (bp)

Gene Insert Sequence:

ATGAAGGCACAGACAGCACTGTCTTTCTTCCTCATTCTCATAACATCTCTGAGTGGATCTCAAGGCATATTCCCTTTGGC

TTTCTTCATTTATGTTCCTATGAATGAACAAATCGTCATTGGAAGACTTGATGAAGATATAATTCTCCCTTCTTCATTTG

AGAGGGGATCCGAAGTCGTAATACACTGGAAGTATCAAGATAGCTATAAGGTTCACAGTTACTACAAAGGCAGTGACCAT

TTGGAAAGCCAAGATCCCAGATATGCAAACAGGACATCCCTTTTCTATAATGAGATTCAAAATGGGAATGCGTCGCTATT

TTTCAGAAGAGTAAGCCTTCTGGACGAAGGAATTTACACCTGCTATGTAGGAACAGCAATTCAAGTGATTACAAACAAAG

TGGTGCTAAAGGTGGGAGTTTTTCTCACACCCGTGATGAAGTATGAAAAGAGGAACACAAACAGCTTCTTAATATGCAGC

GTGTTAAGTGTTTATCCTCGTCCAATTATCACGTGGAAAATGGACAACACACCTATCTCTGAAAACAACATGGAAGAAAC

AGGGTCTTTGGATTCTTTTTCTATTAACAGCCCACTGAATATTACAGGATCAAATTCATCTTATGAATGTACAATTGAAA

ATTCACTGCTGAAGCAAACATGGACAGGGCGCTGGACGATGAAAGATGGCCTTCATAAAATGCAAAGTGAACACGTTTCA

CTCTCATGTCAACCTGTAAATGATTATTTTTCACCAAACCAAGACTTCAAAGTTACTTGGTCCAGAATGAAAAGTGGGAC

TTTCTCTGTCCTGGCTTACTATCTGAGCTCCTCACAAAATACAATTATCAATGAATCCCGATTCTCATGGAACAAAGAGC

TGATAAACCAGAGTGACTTCTCTATGAATTTGATGGATCTTAATCTTTCAGACAGTGGGGAATATTTATGCAATATTTCT

TCGGATGAATATACTTTACTTACCATCCACACAGTGCATGTAGAACCGAGCCAAGAAACAGCTTCCCATAACAAAGGCTT

ATGGATTTTGGTGCCCTCTGCGATTTTGGCAGCTTTTCTGCTGATTTGGAGCGTAAAATGTTGCAGAGCCCAGCTAGAAG

CCAGGAGGAGCAGACACCCTGCTGATGGAGCCCAACAAGAAAGATGTTGTGTCCCTCCTGGTGAGCGCTGTCCCAGTGCA

CCCGATAATGGCGAAGAAAATGTGCCTCTTTCAGGAAAAGTATAG

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

Storage

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

References