CD30L (CD30 ligand), also known as TNFSF8 and CD153, is a cytokine that belongs to the tumor necrosis factor (TNF) superfamily (TNFSF). CD30L/CD153 is a single-pass type II transmebrane glycoprotein consisting of a 37 amino acid (aa) cytoplasmic domain, a 25-aa transmembrane domain (TM) and a 172 aa extracellular domain (ECD).  This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of CD30L expressed on B cell surface plays an inhibitory role in modulating Ig class switch. CD30L induces proliferation of T-cells. It enhances cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of CD30L on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. 

CD30L (CD30 ligand), also known as TNFSF8 and CD153, is a cytokine that belongs to the tumor necrosis factor (TNF) superfamily (TNFSF). CD30L/CD153 is a single-pass type II transmebrane glycoprotein consisting of a 37 amino acid (aa) cytoplasmic domain, a 25-aa transmembrane domain (TM) and a 172 aa extracellular domain (ECD).  This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of CD30L expressed on B cell surface plays an inhibitory role in modulating Ig class switch. CD30L induces proliferation of T-cells. It enhances cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of CD30L on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. 

  

Product Details

 

Gene Symbol: CD30L; CD153; TNFSF8; CD30-L; CD30LG; TNLG3A

  

NCBI Gene ID: 944

 

Uniprot Entry: P32971

 

Construct Details: The recombinant human CD30L-Fc fusion is expressed as a 400 amino acid protein consisting of Gln63 - Asp234 region of CD30L and a Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source: Human cells stably expressing human CD30L-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

QRTDSIPNSP DNVPLKGGNC SEDLLCILKR APFKKSWAYL QVAKHLNKTK LSWNKDGILH 

        70         80         90        100        110        120 

GVRYQDGNLV IQFPGLYFII CQLQFLVQCP NNSVDLKLEL LINKHIKKQA LVTVCESGMQ 

       130        140        150        160        170        180 

TKHVYQNLSQ FLLDYLQVNT TISVNVDTFQ YIDTSTFPLE NVLSIFLYSN SDSTGTHTCP 

       190        200        210        220        230        240 

PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA 

       250        260        270        280        290        300 

KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ 

       310        320        330        340        350        360 

VYTLPPSREE MTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY 

       370        380        390        400 

SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK 

 

M.W.: Calculated molecular mass (kDa): 45.2; Estimated by SDS-PAGE under reducing condition (kDa): 55-60 

 

Calculated PI: 7.97

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 57465

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method 

 

Bioactivity:  Binds to its receptor CD30 and induces CD30-mediated signaling activities. 

 

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.