Human RELT Full-length Gene in Lentivector, Endotoxin-free DNA

Product ID: pLTC-RELT (SKU#: LTP0041)

Price:

$796.00

Description

RELT (receptor expressed in lymphoid tissues) is a single-pass, type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF19L). RELT is primarily expressed in hematopoietic tissues with highest levels in spleen, lymph node, thymus, peripheral blood leukocytes, bone marrow and fetal liver. It is also found in in leukemias and lymphomas. RELT has a 137 amino acid (aa) extracellular domain containing one TNFR cysteinerich repeat and a 247 aa cytoplasmic region that lacks a death domain. The ECD of RELT is most closely related to that of TROY (TNFRSF19) and OX40 (TNFRSF4). RELT has been shown to selectively bind the adaptor protein TNF receptor-associated factor 1 (TRAF1). Overexpression of RELT can activate the NF-kB, p38 and JNK signaling pathways and induces apoptosis. RELT is also capable of stimulating T-cell proliferation in the presence of CD3 signaling. However RELT does not bind any of the TNF superfamily ligands. Two related transmembrane proteins, RELL1 and RELL2, have been identified. Both RELL1 and RELL2 can interact with RELT and may modulate its signaling activity.

Product Details

Gene Symbol: TRLT; TNFRSF19L

NCBI Gene ID: 84957

Uniprot Entry: Q969Z4

Construct Details: Full length human RELT gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

Gene Insert Sequence:

ATGAAGCCAAGTCTGCTGTGCCGGCCCCTGTCCTGCTTCCTTATGCTGCTGCCCTGGCCTCTCGCCACCCTGACATCAAC

AACCCTTTGGCAGTGCCCACCTGGGGAGGAGCCCGACCTGGACCCAGGGCAGGGCACATTATGCAGGCCCTGCCCCCCAG

GCACCTTCTCAGCTGCATGGGGCTCCAGCCCATGCCAGCCCCATGCCCGTTGCAGCCTTTGGAGGAGGCTGGAGGCCCAG

GTGGGCATGGCAACTCGAGATACACTCTGTGGAGACTGCTGGCCTGGGTGGTTTGGGCCTTGGGGGGTTCCCCGCGTTCC

ATGTCAACCATGTTCCTGGGCACCTCTGGGTACTCATGGCTGTGATGAGTGGGGGCGGCGGGCCCGACGTGGCGTGGAGG

TGGCAGCAGGGGCCAGCAGCGGTGGTGAGACACGGCAGCCTGGGAACGGCACCCGGGCAGGTGGCCCAGAGGAGACAGCC

GCCCAGTACGCGGTCATCGCCATCGTCCCTGTCTTCTGCCTCATGGGGCTGTTGGGCATCCTGGTGTGCAACCTCCTCAA

GCGGAAGGGCTACCACTGCACGGCGCACAAGGAGGTCGGGCCCGGCCCTGGAGGTGGAGGCAGTGGAATCAACCCTGCCT

ACCGGACTGAGGATGCCAATGAGGACACCATTGGGGTCCTGGTGCGCTTGATCACAGAGAAGAAAGAGAATGCTGCGGCC

CTGGAGGAGCTGCTGAAAGAGTACCACAGCAAACAGCTGGTGCAGACGAGCCACAGGCCTGTGTCCAAGCTGCCGCCAGC

GCCCCCGAACGTGCCACACATCTGCCCGCACCGCCACCATCTCCACACCGTGCAGGGCCTGGCCTCGCTCTCTGGCCCCT

GCTGCTCCCGCTGTAGCCAGAAGAAGTGGCCCGAGGTGCTGCTGTCCCCTGAGGCTGTAGCCGCCACTACTCCTGTTCCC

AGCCTTCTGCCTAACCCGACCAGGGTTCCCAAGGCCGGGGCCAAGGCAGGGCGTCAGGGCGAGATCACCATCTTGTCTGT

GGGCAGGTTCCGCGTGGCTCGAATTCCTGAGCAGCGGACAAGTTCAATGGTGTCTGAGGTGAAGACCATCACGGAGGCTG

GGCCCTCGTGGGGTGATCTCCCTGACTCCCCACAGCCTGGCCTCCCCCCTGAGCAGCAGGCCCTGCTAGGAAGTGGCGGA

AGCCGTACAAAGTGGCTGAAGCCCCCAGCAGAGAACAAGGCCGAGGAGAACCGCTATGTGGTCCGGCTAAGTGAGAGCAA

CCTGGTCATCTGA

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

Storage

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

References