CD70, also known as CD27L (CD27 Ligand), is a member of the tumor necrosis factor superfamily (TNFSF7). CD70 is a single-pass type II transmebrane glycoprotein consisting of a short cytoplasmic domain (1-17aa), a transmembrane domain (TM: 18-38 aa) and a 155aa extracellular domain (ECD: 39-193 aa).  CD70 exists as a trimer that is found on activated T-and B-lymphocytes and mature dendritic cells. The expression of CD70 is induced by antigen receptor activation in B cells and at low levels in mouse T cells.  CD70 interacts with CD27, a member of the TNF receptor superfamily (TNFRSF7) that is expressed on natural killer (NK) cells and subsets of T and B cells. Ligation with CD27 on T cells provides co-stimulatory signals for T cell proliferation, clonal expansion and the promotion of effector T cell function. On NK cells, ligation of CD70 induces proliferation and IFN-gamma production. CD70 expression on tumor cells coupled with its highly restricted expression on normal tissues makes CD70 an attractive target for immunotherapy.

CD70, also known as CD27L (CD27 Ligand), is a member of the tumor necrosis factor superfamily (TNFSF7). CD70 is a single-pass type II transmebrane glycoprotein consisting of a short cytoplasmic domain (1-17aa), a transmembrane domain (TM: 18-38 aa) and a 155aa extracellular domain (ECD: 39-193 aa).  CD70 exists as a trimer that is found on activated T-and B-lymphocytes and mature dendritic cells. The expression of CD70 is induced by antigen receptor activation in B cells and at low levels in mouse T cells.  CD70 interacts with CD27, a member of the TNF receptor superfamily (TNFRSF7) that is expressed on natural killer (NK) cells and subsets of T and B cells. Ligation with CD27 on T cells provides co-stimulatory signals for T cell proliferation, clonal expansion and the promotion of effector T cell function. On NK cells, ligation of CD70 induces proliferation and IFN-gamma production. CD70 expression on tumor cells coupled with its highly restricted expression on normal tissues makes CD70 an attractive target for immunotherapy.

  

Product Details

 

Gene Symbol: CD70; CD27L; CD27LG; TNFSF7; TNLG8A

  

NCBI Gene ID: 970

 

Uniprot Entry: P32970

 

Construct Details: Full length human CD70 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter and a marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Sequence:  

ATGCCGGAGGAGGGTTCGGGCTGCTCGGTGCGGCGCAGGCCCTATGGGTGCGTCCTGCGGGCTGCTTTGG

TCCCATTGGTCGCGGGCTTGGTGATCTGCCTCGTGGTGTGCATCCAGCGCTTCGCACAGGCTCAGCAGCA

GCTGCCGCTCGAGTCACTTGGGTGGGACGTAGCTGAGCTGCAGCTGAATCACACAGGACCTCAGCAGGAC

CCCAGGCTATACTGGCAGGGGGGCCCAGCACTGGGCCGCTCCTTCCTGCATGGACCAGAGCTGGACAAGG

GGCAGCTACGTATCCATCGTGATGGCATCTACATGGTACACATCCAGGTGACGCTGGCCATCTGCTCCTC

CACGACGGCCTCCAGGCACCACCCCACCACCCTGGCCGTGGGAATCTGCTCTCCCGCCTCCCGTAGCATC

AGCCTGCTGCGTCTCAGCTTCCACCAAGGTTGTACCATTGCCTCCCAGCGCCTGACGCCCCTGGCCCGAG

GGGACACACTCTGCACCAACCTCACTGGGACACTTTTGCCTTCCCGAAACACTGATGAGACCTTCTTTGG

AGTGCAGTGGGTGCGCCCCTGA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

1. Immunol. Today 15:307 (1994)

2. Proc. Natl. Acad. Sci. 92:11249 (1995)

3. J. Immunol. 164:1741 (2000)

4. Nature Reviews Immunol. 3:609 (2003)

5. J. Immunol. 169:33 (2003)

 

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTP2530-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.