4-1BBL (4-1BB ligand), also known as CD137L or TNFSF9, is single pass type II transmembrane protein that belongs to the TNF superfamily (TNFSF).  4-1BBL is a  a bidirectional signal transducer that acts as a high affinity ligand for 4-1BB/CD137/TNFRSF9, a member of the TNF-receptor superfamily (TNFRSF).  Members of the TNFRSF have been shown to play critical roles in regulating cellular activation, differentiation and apoptosis.  The extracellular domain (ECD) of 4-1BBL has a jellyroll, β-sandwich tertiary structure that is similar to other TNFSF members. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated T cells, neurons and astrocytes.  A soluble active form of 4-1BBL is detected in humans, presumably generated by MMP activity. 4-1BBL may signal through both 4-1BB and itself as its cytoplasmic tail participates in reverse signaling to induce apoptosis in T cells and secretion of cytokines such as IL6 and TNFα by monocytes. 4-1BBL/4-1BB signaling is involved in the antigen presentation process and in the generation of cytotoxic T cells.  4-1BBL/4-1BB interaction also provides a co-stimulatory signal to T cells, and increases T cell proliferation and cytokines production. 4-1BBL may play a key role in T cell recall response and maintaining T cell numbers. 4-1BBL is required for the optimal CD8 responses in CD8 T cells. 4-1BBL is expressed on cancer cells, and is thought to be involved in T cell-tumor cell interaction.  4-1BBL may also play a role in interactions between T-cells and B-cells/macrophages.  4-1BBL is implicated in cancers, infectious diseases and autoimmune diseases.

4-1BBL (4-1BB ligand), also known as CD137L or TNFSF9, is single pass type II transmembrane protein that belongs to the TNF superfamily (TNFSF).  4-1BBL is a  a bidirectional signal transducer that acts as a high affinity ligand for 4-1BB/CD137/TNFRSF9, a member of the TNF-receptor superfamily (TNFRSF).  Members of the TNFRSF have been shown to play critical roles in regulating cellular activation, differentiation and apoptosis.  The extracellular domain (ECD) of 4-1BBL has a jellyroll, β-sandwich tertiary structure that is similar to other TNFSF members. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated T cells, neurons and astrocytes.  A soluble active form of 4-1BBL is detected in humans, presumably generated by MMP activity. 4-1BBL may signal through both 4-1BB and itself as its cytoplasmic tail participates in reverse signaling to induce apoptosis in T cells and secretion of cytokines such as IL6 and TNFα by monocytes. 4-1BBL/4-1BB signaling is involved in the antigen presentation process and in the generation of cytotoxic T cells.  4-1BBL/4-1BB interaction also provides a co-stimulatory signal to T cells, and increases T cell proliferation and cytokines production. 4-1BBL may play a key role in T cell recall response and maintaining T cell numbers. 4-1BBL is required for the optimal CD8 responses in CD8 T cells. 4-1BBL is expressed on cancer cells, and is thought to be involved in T cell-tumor cell interaction.  4-1BBL may also play a role in interactions between T-cells and B-cells/macrophages.  4-1BBL is implicated in cancers, infectious diseases and autoimmune diseases.

  

Product Details

 

Gene Symbol: 4-1BBL; TNFSF9; CD137L; TNLG5A; 4-1BB-L

  

NCBI Gene ID: 8744

 

Uniprot Entry: P41273

 

Construct Details: The recombinant human 4-1BBL-Fc is expressed as a 412-amino acid protein consisting of  Arg71 -  Glu254 region of 4-1BBL with a Fc-fusion from human IgG1, which exists as a dimer under non-reducing conditions.   

   

Source: Human cells stably expressing human 4-1BBL-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

REGPELSPDD PAGLLDLRQG MFAQLVAQNV LLIDGPLSWY SDPGLAGVSL TGGLSYKEDT 

        70         80         90        100        110        120 

KELVVAKAGV YYVFFQLELR RVVAGEGSGS VSLALHLQPL RSAAGAAALA LTVDLPPASS 

       130        140        150        160        170        180 

EARNSAFGFQ GRLLHLSAGQ RLGVHLHTEA RARHAWQLTQ GATVLGLFRV TPEIPAGLPS 

       190        200        210        220        230        240 

PRSESTGTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF 

       250        260        270        280        290        300 

NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT 

       310        320        330        340        350        360 

ISKAKGQPRE PQVYTLPPSR EEMTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP 

       370        380        390        400        410 

PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK 

  

M.W.: Calculated molecular mass (kDa): 45; Estimated by SDS-PAGE under reducing condition (kDa): ~45-50 with 2 distinct high MW bands around 75 kDa  and 95 kDa.

 

Calculated PI: 6.68

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 52745

 

Purity: >85% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to its receptor 4-1BB (SKU#FCL0100) with high affinity and induces its signaling activity.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

1. Eur. J. Immunol. 24:2219 (1994)

2. J. Immunol. 160:2488 (1998)

3. J. Immunol. 167:4059 (2001)

4. Immunol Rev. 229: 192 (2009)

5. J. Biol. Chem. 285:9202 (2010) 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.