CD70, also known as CD27L (CD27 Ligand), is a member of the tumor necrosis factor superfamily (TNFSF7). CD70 is a single-pass type II transmebrane glycoprotein consisting of a short cytoplasmic domain (1-17aa), a transmembrane domain (TM: 18-38 aa) and a 155aa extracellular domain (ECD: 39-193 aa).  CD70 exists as a trimer that is found on activated T-and B-lymphocytes and mature dendritic cells. The expression of CD70 is induced by antigen receptor activation in B cells and at low levels in mouse T cells.  CD70 interacts with CD27, a member of the TNF receptor superfamily (TNFRSF7) that is expressed on natural killer (NK) cells and subsets of T and B cells. Ligation with CD27 on T cells provides co-stimulatory signals for T cell proliferation, clonal expansion and the promotion of effector T cell function. On NK cells, ligation of CD70 induces proliferation and IFN-gamma production. CD70 expression on tumor cells coupled with its highly restricted expression on normal tissues makes CD70 an attractive target for immunotherapy.

CD70, also known as CD27L (CD27 Ligand), is a member of the tumor necrosis factor superfamily (TNFSF7). CD70 is a single-pass type II transmebrane glycoprotein consisting of a short cytoplasmic domain (1-17aa), a transmembrane domain (TM: 18-38 aa) and a 155aa extracellular domain (ECD: 39-193 aa).  CD70 exists as a trimer that is found on activated T-and B-lymphocytes and mature dendritic cells. The expression of CD70 is induced by antigen receptor activation in B cells and at low levels in mouse T cells.  CD70 interacts with CD27, a member of the TNF receptor superfamily (TNFRSF7) that is expressed on natural killer (NK) cells and subsets of T and B cells. Ligation with CD27 on T cells provides co-stimulatory signals for T cell proliferation, clonal expansion and the promotion of effector T cell function. On NK cells, ligation of CD70 induces proliferation and IFN-gamma production. CD70 expression on tumor cells coupled with its highly restricted expression on normal tissues makes CD70 an attractive target for immunotherapy.

  

Product Details

 

Gene Symbol: CD70; CD27L; CD27LG; TNFSF7; TNLG8A

  

NCBI Gene ID: 970

 

Uniprot Entry: P32970

 

Construct Details: The recombinant human CD70-Fc is expressed as a 384-amino acid protein consisting of Gln39 -  Pro193 region of CD70 (UniProt accession #P32970) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.   

 

Source:  Human cells stably expressing CD70-Fc growing in chemical-defined media with no animal components or antibiotics 

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

QRFAQAQQQL PLESLGWDVA ELQLNHTGPQ QDPRLYWQGG PALGRSFLHG PELDKGQLRI 

        70         80         90        100        110        120 

HRDGIYMVHI QVTLAICSST TASRHHPTTL AVGICSPASR SISLLRLSFH QGCTIASQRL  

       130        140        150        160        170        180 

TPLARGDTLC TNLTGTLLPS RNTDETFFGV QWVRPRTDKT HTCPPCPAPE LLGGPSVFLF  

       190        200        210        220        230        240 

PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV  

       250        260        270        280        290        300 

SVLTVLHQDW LNGKEYKCKV SNKALPAPIE KTISKAKGQP REPQVYTLPP SREEMTKNQV 

       310        320        330        340        350        360 

SLTCLVKGFY PSDIAVEWES NGQPENNYKT TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF 

       370        380 

SCSVMHEALH NHYTQKSLSL SPGK 

 

M.W.: Calculated molecular mass (kDa): 43; Estimated by SDS-PAGE under reducing condition (kDa): 50-60

 

Calculated PI: 8.46

 

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm): 55515

 

Purity: >90% judged by SDS-PAGE under reducing condition (multiple bands, see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to its receptor CD27-Fc (SKU# FCL0102) by ELISA and  stimulates T cell proliferation in the presence of anti-CD3e antibody. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

1. Immunol. Today 15:307 (1994)

2. Proc. Natl. Acad. Sci. 92:11249 (1995)

3. J. Immunol. 164:1741 (2000)

4. Nature Reviews Immunol. 3:609 (2003)

5. J. Immunol. 169:33 (2003)

 

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2530B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.