Axl, also known as Ufo and Ark, is a single-pass type I transmembrane glycoprotein in the TAM receptor tyrosine kinase (RTK) family. Like other family members Tyro3 and Mer, Axl contains two immunoglobulin­like (Ig) domains and two fibronectin type III domains in the extracellular region and a cytoplasmic tyrosine kinase domain. The TAM receptors are involved in regulation of immune response, cell survival and migration as well as tumorigenesis and metastasis. Axl binds to its ligand Gas6 (growth arrest specific protein 6), which triggers tyrosine auto-phosphorylation and activation of Axl. Axl induces a wide range of activities including platelet aggregation and thrombus formation, macrophage and dendritic cell phagocytosis, NK cell development, and angiogenesis. Axl is upregulated in various disease states, such as in multiple sclerosis (MS) lesions. Axl contributes to vascular remodeling and inflammatory cell infiltration. Axl acts as a cellular entry receptor for Gas6­opsonized lentiviruses. Axl also contributes to tumor cell survival, migration, invasion, metastasis and chemosensitivity. Axl is highly expressed in solid cancers and mediates multiple oncogenic phenotypes. Axl expression correlates with poor prognosis in several cancers. A soluble form of the Axl ectodomain, which is shed by proteolytic cleavage, retains the ability to bind Gas6 and blocks its signaling, showing the therapeutic potential in the inhibition of metastatic tumor progression.

Axl, also known as Ufo and Ark, is a single-pass type I transmembrane glycoprotein in the TAM receptor tyrosine kinase (RTK) family. Like other family members Tyro3 and Mer, Axl contains two immunoglobulin­like (Ig) domains and two fibronectin type III domains in the extracellular region and a cytoplasmic tyrosine kinase domain. The TAM receptors are involved in regulation of immune response, cell survival and migration as well as tumorigenesis and metastasis. Axl binds to its ligand Gas6 (growth arrest specific protein 6), which triggers tyrosine auto-phosphorylation and activation of Axl. Axl induces a wide range of activities including platelet aggregation and thrombus formation, macrophage and dendritic cell phagocytosis, NK cell development, and angiogenesis. Axl is upregulated in various disease states, such as in multiple sclerosis (MS) lesions. Axl contributes to vascular remodeling and inflammatory cell infiltration. Axl acts as a cellular entry receptor for Gas6­opsonized lentiviruses. Axl also contributes to tumor cell survival, migration, invasion, metastasis and chemosensitivity. Axl is highly expressed in solid cancers and mediates multiple oncogenic phenotypes. Axl expression correlates with poor prognosis in several cancers. A soluble form of the Axl ectodomain, which is shed by proteolytic cleavage, retains the ability to bind Gas6 and blocks its signaling, showing the therapeutic potential in the inhibition of metastatic tumor progression.

 

Product Details

 

Gene Symbol: AXL; UFO; JTK11

 

NCBI Gene ID: 558

 

Uniprot Entry: P30530

 

Construct Details: Full length human AXL/UFO gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Gene Insert Sequence:  

ATGGCGTGGCGGTGCCCCAGGATGGGCAGGGTCCCGCTGGCCTGGTGCTTGGCGCTGTGCGGCTGGGCGTGCATGGCCCC

CAGGGGCACGCAGGCTGAAGAAAGTCCCTTCGTGGGCAACCCAGGGAATATCACAGGTGCCCGGGGACTCACGGGCACCC

TTCGGTGTCAGCTCCAGGTTCAGGGAGAGCCCCCCGAGGTACATTGGCTTCGGGATGGACAGATCCTGGAGCTCGCGGAC

AGCACCCAGACCCAGGTGCCCCTGGGTGAGGATGAACAGGATGACTGGATAGTGGTCAGCCAGCTCAGAATCACCTCCCT

GCAGCTTTCCGACACGGGACAGTACCAGTGTTTGGTGTTTCTGGGACATCAGACCTTCGTGTCCCAGCCTGGCTATGTTG

GGCTGGAGGGCTTGCCTTACTTCCTGGAGGAGCCCGAAGACAGGACTGTGGCCGCCAACACCCCCTTCAACCTGAGCTGC

CAAGCTCAGGGACCCCCAGAGCCCGTGGACCTACTCTGGCTCCAGGATGCTGTCCCCCTGGCCACGGCTCCAGGTCACGG

CCCCCAGCGCAGCCTGCATGTTCCAGGGCTGAACAAGACATCCTCTTTCTCCTGCGAAGCCCATAACGCCAAGGGGGTCA

CCACATCCCGCACAGCCACCATCACAGTGCTCCCCCAGCAGCCCCGTAACCTCCACCTGGTCTCCCGCCAACCCACGGAG

CTGGAGGTGGCTTGGACTCCAGGCCTGAGCGGCATCTACCCCCTGACCCACTGCACCCTGCAGGCTGTGCTGTCAGACGA

TGGGATGGGCATCCAGGCGGGAGAACCAGACCCCCCAGAGGAGCCCCTCACCTCGCAAGCATCCGTGCCCCCCCATCAGC

TTCGGCTAGGCAGCCTCCATCCTCACACCCCTTATCACATCCGCGTGGCATGCACCAGCAGCCAGGGCCCCTCATCCTGG

ACCCACTGGCTTCCTGTGGAGACGCCGGAGGGAGTGCCCCTGGGCCCCCCTGAGAACATTAGTGCTACGCGGAATGGGAG

CCAGGCCTTCGTGCATTGGCAAGAGCCCCGGGCGCCCCTGCAGGGTACCCTGTTAGGGTACCGGCTGGCGTATCAAGGCC

AGGACACCCCAGAGGTGCTAATGGACATAGGGCTAAGGCAAGAGGTGACCCTGGAGCTGCAGGGGGACGGGTCTGTGTCC

AATCTGACAGTGTGTGTGGCAGCCTACACTGCTGCTGGGGATGGACCCTGGAGCCTCCCAGTACCCCTGGAGGCCTGGCG

CCCAGGGCAAGCACAGCCAGTCCACCAGCTGGTGAAGGAACCTTCAACTCCTGCCTTCTCGTGGCCCTGGTGGTATGTAC

TGCTAGGAGCAGTCGTGGCCGCTGCCTGTGTCCTCATCTTGGCTCTCTTCCTTGTCCACCGGCGAAAGAAGGAGACCCGT

TATGGAGAAGTGTTTGAACCAACAGTGGAAAGAGGTGAACTGGTAGTCAGGTACCGCGTGCGCAAGTCCTACAGTCGTCG

GACCACTGAAGCTACCTTGAACAGCTTGGGCATCAGTGAAGAGCTGAAGGAGAAGCTGCGGGATGTGATGGTGGACCGGC

ACAAGGTGGCCCTGGGGAAGACTCTGGGAGAGGGAGAGTTTGGAGCTGTGATGGAAGGCCAGCTCAACCAGGACGACTCC

ATCCTCAAGGTGGCTGTGAAGACGATGAAGATTGCCATCTGCACGAGGTCAGAGCTGGAGGATTTCCTGAGTGAAGCGGT

CTGCATGAAGGAATTTGACCATCCCAACGTCATGAGGCTCATCGGTGTCTGTTTCCAGGGTTCTGAACGAGAGAGCTTCC

CAGCACCTGTGGTCATCTTACCTTTCATGAAACATGGAGACCTACACAGCTTCCTCCTCTATTCCCGGCTCGGGGACCAG

CCAGTGTACCTGCCCACTCAGATGCTAGTGAAGTTCATGGCAGACATCGCCAGTGGCATGGAGTATCTGAGTACCAAGAG

ATTCATACACCGGGACCTGGCGGCCAGGAACTGCATGCTGAATGAGAACATGTCCGTGTGTGTGGCGGACTTCGGGCTCT

CCAAGAAGATCTACAATGGGGACTACTACCGCCAGGGACGTATCGCCAAGATGCCAGTCAAGTGGATTGCCATTGAGAGT

CTAGCTGACCGTGTCTACACCAGCAAGAGCGATGTGTGGTCCTTCGGGGTGACAATGTGGGAGATTGCCACAAGAGGCCA

AACCCCATATCCGGGCGTGGAGAACAGCGAGATTTATGACTATCTGCGCCGGGGAAATCGCCTGAAGCAGCCTGCGGACT

GTCTGGATGGACTGTATGCCTTGATGTCGCGGTGCTGGGAGCTAAATCCCCAGGACCGGCCAAGTTTTACAGAGCTGCGG

GAAGATTTGGAGAACACACTGAAGGCCTTGCCTCCTGCCCAGGAGCCTGACGAAATCCTCTATGTCAACATGGATGAGGG

TGGAGGTTATCCTGAACCCCCTGGAGCTGCAGGAGGAGCTGACCCCCCAACCCAGCCAGACCCTAAGGATTCCTGTAGCT

GCCTCACTGCGGCTGAGGTCCATCCTGCTGGACGCTATGTCCTCTGCCCTTCCACAACCCCTAGCCCCGCTCAGCCTGCT

GATAGGGGCTCCCCAGCAGCCCCAGGGCAGGAGGATGGTGCCTGA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

Formulation: Lentivector encoded and pre-packaged viral particles (typical titer 10⁶ - 10⁷ IFU/ml) in the conditional medium (serum-free) from HEK293 cells that have been transfected with the lentivector gene clone and the LentiPAK DNA mix

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Important Safety Information: With the safety features in place, our lentiviral vectors and viral particles can be employed in standard Biosafety Level 2 tissue culture facilities and should be treated with the same level of caution as any other potentially infectious agent. Any investigator who purchases our lentiviral/retroviral products & services is responsible for following Biosafety Level 2 requirements on the handling of viral particles. For more information on Biosafety Level 2 agents and practices, please refer to NIH’s “Biosafety Considerations for Research with Lentiviral Vectors”.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C for immediate use.  Upon receipt, centrifuge the vial briefly before opening.  Store at –80°C or lower and the product is stable for 3 months.  Avoid repeated freeze-thaw cycles.

 

 

The product should be employed in a Biosafety Level 2 tissue culture facility.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Mol. Cell. Biol. 11:5016 (1991).  

2. J. Biol. Chem. 270:551 (1995).  

3. Nature 373:623 (1995).

4. Cancer Res. 65:9294 (2005).  

5. Cancer Res. 70:7570 (2010).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTV1057-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.