DcR3 (death decoy receptor), also known as decoy receptor for Fas ligand, TR6 and M68, is a member of the TNF receptor superfamily (TNFRSF6B).  Like osteoprotegerin (OPG), DcR3 lacks a transmembrane sequence and is a secreted protein that contains 4 TNFR cysteine-rich repeats. Expression of DcR3 has been reported in a wide array of normal tissues as well as primary tumors and tumor cell lines. DcR3 is detected in fetal lung, brain and liver and in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung.  DcR3 is highly expressed in primary tumors from colon, stomach, rectum, and esophagus as well as in SW480 colon cancer cell line. DcR3 was shown to bind with Fas ligand (FasL) and LIGHT and TNF- like molecule 1A (TL1A). The principal function of DcR3 is the inhibition of FasL­ and LIGHT-­induced apoptosis. In addition to DcR1, DcR2 and OPG, DcR3 is another TNFRSF member that modulates cytotoxic ligands-induced apoptosis. Overexpression of DcR3 may be a mechanism by which certain tumors escape immune­cytotoxic attack. DcR3 may also modulate CNS- autoimmunity by interfering with Th17 responses via the blockade of Fas-FasL interaction.

DcR3 (death decoy receptor), also known as decoy receptor for Fas ligand, TR6 and M68, is a member of the TNF receptor superfamily (TNFRSF6B).  Like osteoprotegerin (OPG), DcR3 lacks a transmembrane sequence and is a secreted protein that contains 4 TNFR cysteine-rich repeats. Expression of DcR3 has been reported in a wide array of normal tissues as well as primary tumors and tumor cell lines. DcR3 is detected in fetal lung, brain and liver and in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung.  DcR3 is highly expressed in primary tumors from colon, stomach, rectum, and esophagus as well as in SW480 colon cancer cell line. DcR3 was shown to bind with Fas ligand (FasL) and LIGHT and TNF- like molecule 1A (TL1A). The principal function of DcR3 is the inhibition of FasL­ and LIGHT-­induced apoptosis. In addition to DcR1, DcR2 and OPG, DcR3 is another TNFRSF member that modulates cytotoxic ligands-induced apoptosis. Overexpression of DcR3 may be a mechanism by which certain tumors escape immune­cytotoxic attack. DcR3 may also modulate CNS- autoimmunity by interfering with Th17 responses via the blockade of Fas-FasL interaction.

  

Product Details

 

Gene Symbol: TNFRSF6B; M68; TR6; DCR3; M68E; DJ583P15.1.1; UNQ186/PRO212

  

NCBI Gene ID: 8771

 

Uniprot Entry: Q95407

 

Construct Details: Full length human DcR3 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Sequence:  

ATGAGGGCGCTGGAGGGGCCAGGCCTGTCGCTGCTGTGCCTGGTGTTGGCGCTGCCTGCCCTGCTGCCGGTGCCGGCTGT

ACGCGGAGTGGCAGAAACACCCACCTACCCCTGGCGGGACGCAGAGACAGGGGAGCGGCTGGTGTGCGCCCAGTGCCCCC

CAGGCACCTTTGTGCAGCGGCCGTGCCGCCGAGACAGCCCCACGACGTGTGGCCCGTGTCCACCGCGCCACTACACGCAG

TTCTGGAACTACCTAGAGCGCTGCCGCTACTGCAACGTCCTCTGCGGGGAGCGTGAGGAGGAGGCACGGGCTTGCCACGC

CACCCACAACCGTGCCTGCCGCTGCCGCACCGGCTTCTTCGCGCACGCTGGTTTCTGCTTGGAGCACGCATCGTGTCCAC

CTGGTGCCGGCGTGATTGCCCCGGGCACCCCCAGCCAGAACACGCAGTGCCAGCCGTGCCCCCCAGGCACCTTCTCAGCC

AGCAGCTCCAGCTCAGAGCAGTGCCAGCCCCACCGCAACTGCACGGCCCTGGGCCTGGCCCTCAATGTGCCAGGCTCTTC

CTCCCATGACACCCTGTGCACCAGCTGCACTGGCTTCCCCCTCAGCACCAGGGTACCAGGAGCTGAGGAGTGTGAGCGTG

CCGTCATCGACTTTGTGGCTTTCCAGGACATCTCCATCAAGAGGCTGCAGCGGCTGCTGCAGGCCCTCGAGGCCCCGGAG

GGCTGGGGTCCGACACCAAGGGCGGGCCGCGCGGCCTTGCAGCTGAAGCTGCGTCGGCGGCTCACGGAGCTCCTGGGGGC

GCAGGACGGGGCGCTGCTGGTGCGGCTGCTGCAGGCGCTGCGCGTGGCCAGGATGCCCGGGCTGGAGCGGAGCGTCCGTG

AGCGCTTCCTCCCTGTGCACTGA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

1. Nature 396:699 (1998).

2. J. Biochem. Chem. 274:13733 (1999).

3. Proc. Natl. Acad. Sci. 97:1230 (2000).

4. J Am Soc Nephrol. 19: 1025 (2008). 

5. J Neuroimmunol. 209: 57 (2009).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTPSF55-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.