DR3 (death receptor 3), also known as AIR, LARD, WSL­1, APO3, TRAMP and TR3, is a single pass type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF25). DR3 contains an extracellular region with 4 TNFR cysteine­rich repeats and a cytoplasmic region with 1 death domain (DD) that is also found in other 5 TNFRSF members. DR3 is most closely related to TNFR1 and FAS/CD95, sharing 29% and 23% amino acid sequence identity, respectively.  DR3 is abundantly expressed in thymocytes and lymphocytes and detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. It is also found in the prostate. Naïve B and T cells express multiple truncated DR3 isoforms that lack the transmembrane domain. Upon T cell activation, expression of the transmembrane isoform 1 of DR3 predominates. DR3 interacts directly with the adapter protein TRADD to mediate activation of NF-κB and induce apoptosis. DR3 may play a role in regulating lymphocyte homeostasis. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. TL1A/TNFSF15, a TNF superfamily ligand, has been shown to bind and activate DR3. On T cells, TL1A induces a costimulatory signal to increase IL­2 responsiveness and the secretion of proinflammatory cytokines. TL1A has also been shown to induce caspase activity and apoptosis.

DR3 (death receptor 3), also known as AIR, LARD, WSL­1, APO3, TRAMP and TR3, is a single pass type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF25). DR3 contains an extracellular region with 4 TNFR cysteine­rich repeats and a cytoplasmic region with 1 death domain (DD) that is also found in other 5 TNFRSF members. DR3 is most closely related to TNFR1 and FAS/CD95, sharing 29% and 23% amino acid sequence identity, respectively.  DR3 is abundantly expressed in thymocytes and lymphocytes and detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. It is also found in the prostate. Naïve B and T cells express multiple truncated DR3 isoforms that lack the transmembrane domain. Upon T cell activation, expression of the transmembrane isoform 1 of DR3 predominates. DR3 interacts directly with the adapter protein TRADD to mediate activation of NF-κB and induce apoptosis. DR3 may play a role in regulating lymphocyte homeostasis. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. TL1A/TNFSF15, a TNF superfamily ligand, has been shown to bind and activate DR3. On T cells, TL1A induces a costimulatory signal to increase IL­2 responsiveness and the secretion of proinflammatory cytokines. TL1A has also been shown to induce caspase activity and apoptosis.

  

Product Details

 

Gene Symbol: TNFRSF25; AIR; TR3; DDR3; LARD; APO-3; TRAMP; WSL; WSL1; WSL-1; WSL-LR; TNFRSF12; UNQ455/PRO779

  

NCBI Gene ID: 8718

 

Uniprot Entry: Q93038

 

Construct Details: Full length human DR3 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Sequence:  

ATGGAGCAGCGGCCGCGGGGCTGCGCGGCGGTGGCGGCGGCGCTCCTCCTGGTGCTGCTGGGGGCCCGGGCCCAGGGCGG

CACTCGTAGCCCCAGGTGTGACTGTGCCGGTGACTTCCACAAGAAGATTGGTCTGTTTTGTTGCAGAGGCTGCCCAGCGG

GGCACTACCTGAAGGCCCCTTGCACGGAGCCCTGCGGCAACTCCACCTGCCTTGTGTGTCCCCAAGACACCTTCTTGGCC

TGGGAGAACCACCATAATTCTGAATGTGCCCGCTGCCAGGCCTGTGATGAGCAGGCCTCCCAGGTGGCGCTGGAGAACTG

TTCAGCAGTGGCCGACACCCGCTGTGGCTGTAAGCCAGGCTGGTTTGTGGAGTGCCAGGTCAGCCAATGTGTCAGCAGTT

CACCCTTCTACTGCCAACCATGCCTAGACTGCGGGGCCCTGCACCGCCACACACGGCTACTCTGTTCCCGCAGAGATACT

GACTGTGGGACCTGCCTGCCTGGCTTCTATGAACATGGCGATGGCTGCGTGTCCTGCCCCACGAGCACCCTGGGGAGCTG

TCCAGAGCGCTGTGCCGCTGTCTGTGGCTGGAGGCAGATGTTCTGGGTCCAGGTGCTCCTGGCTGGCCTTGTGGTCCCCC

TCCTGCTTGGGGCCACCCTGACCTACACATACCGCCACTGCTGGCCTCACAAGCCCCTGGTTACTGCAGATGAAGCTGGG

ATGGAGGCTCTGACCCCACCACCGGCCACCCATCTGTCACCCTTGGACAGCGCCCACACCCTTCTAGCACCTCCTGACAG

CAGTGAGAAGATCTGCACCGTCCAGTTGGTGGGTAACAGCTGGACCCCTGGCTACCCCGAGACCCAGGAGGCGCTCTGCC

CGCAGGTGACATGGTCCTGGGACCAGTTGCCCAGCAGAGCTCTTGGCCCCGCTGCTGCGCCCACACTCTCGCCAGAGTCC

CCAGCCGGCTCGCCAGCCATGATGCTGCAGCCGGGCCCGCAGCTCTACGACGTGATGGACGCGGTCCCAGCGCGGCGCTG

GAAGGAGTTCGTGCGCACGCTGGGGCTGCGCGAGGCAGAGATCGAAGCCGTGGAGGTGGAGATCGGCCGCTTCCGAGACC

AGCAGTACGAGATGCTCAAGCGCTGGCGCCAGCAGCAGCCCGCGGGCCTCGGAGCCGTTTACGCGGCCCTGGAGCGCATG

GGGCTGGACGGCTGCGTGGAAGACTTGCGCAGCCGCCTGCAGCGCGGCCCGTGA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

1. Science 274:990 (1996).

2. Nature 384:372 (1996).

3. Curr. Biol. 6:1669 (1996).

4. Immunity 6:79 (1997).

5. Immunity 16:479 (2002).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTPSF56-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.