DR6 (death receptor 6), also known as CD358, is a single-pass, type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF21). DR6 contains an extracellular domain (ECD) with 4 TNFR cysteine-rich repeats and a palmitylated cytoplasmic region with 1 death domain (DD). DR6 is expressed in most human tissues with the highest levels in heart, brain, placenta, pancreas, thymus, lymph node and several non-lymphoid cancer cell lines. Among hematopoietic cells, DR6 is expressed on monocytes, resting CD4+ T cells, and pro-, pre-, and naïve B cells. DR6 interacts with the adaptor protein TRADD and mediates signal transduction through its death domain. Expression of DR6 in mammalian cells induces the activation of NF-κB and JNK pathways as well as apoptosis. DR6 acts as an important regulatory molecule in T cell activation, and is involved in inflammation. DR6-defieicent mice have enhanced CD4+ T cell proliferation and are resistant to induced airway inflammation and experimental autoimmune encephalitis. DR6 is constitutively expressed on some prostate cancer cells and can be induced by TNF-α on other cytokines. DR6 is uniquely cleaved from tumor cell surface by the membrane- associated matrix metalloproteinase that is associated with tumor malignancy. DR6 is also expressed on developing neurons where it can bind APP fragment that is generated following deprivation of neurotrophic factors. APP binding to DR6 triggers DR6-mediated axonal pruning..

DR6 (death receptor 6), also known as CD358, is a single-pass, type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF21). DR6 contains an extracellular domain (ECD) with 4 TNFR cysteine-rich repeats and a palmitylated cytoplasmic region with 1 death domain (DD). DR6 is expressed in most human tissues with the highest levels in heart, brain, placenta, pancreas, thymus, lymph node and several non-lymphoid cancer cell lines. Among hematopoietic cells, DR6 is expressed on monocytes, resting CD4+ T cells, and pro-, pre-, and naïve B cells. DR6 interacts with the adaptor protein TRADD and mediates signal transduction through its death domain. Expression of DR6 in mammalian cells induces the activation of NF-κB and JNK pathways as well as apoptosis. DR6 acts as an important regulatory molecule in T cell activation, and is involved in inflammation. DR6-defieicent mice have enhanced CD4+ T cell proliferation and are resistant to induced airway inflammation and experimental autoimmune encephalitis. DR6 is constitutively expressed on some prostate cancer cells and can be induced by TNF-α on other cytokines. DR6 is uniquely cleaved from tumor cell surface by the membrane- associated matrix metalloproteinase that is associated with tumor malignancy. DR6 is also expressed on developing neurons where it can bind APP fragment that is generated following deprivation of neurotrophic factors. APP binding to DR6 triggers DR6-mediated axonal pruning..

  

Product Details

 

Gene Symbol: TNFRSF21; CD358; BM-018; UNQ437/PRO868

  

NCBI Gene ID: 27242

 

Uniprot Entry: O75509

 

Construct Details: Full length human DR6 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Sequence:  

ATGGGGACCTCTCCGAGCAGCAGCACCGCCCTCGCCTCCTGCAGCCGCATCGCCCGCCGAGCCACAGCCACGATGATCGC

GGGCTCCCTTCTCCTGCTTGGATTCCTTAGCACCACCACAGCTCAGCCAGAACAGAAGGCCTCGAATCTCATTGGCACAT

ACCGCCATGTTGACCGTGCCACCGGCCAGGTGCTAACCTGTGACAAGTGTCCAGCAGGAACCTATGTCTCTGAGCATTGT

ACCAACACAAGCCTGCGCGTCTGCAGCAGTTGCCCTGTGGGGACCTTTACCAGGCATGAGAATGGCATAGAGAAATGCCA

TGACTGTAGTCAGCCATGCCCATGGCCAATGATTGAGAAATTACCTTGTGCTGCCTTGACTGACCGAGAATGCACTTGCC

CACCTGGCATGTTCCAGTCTAACGCTACCTGTGCCCCCCATACGGTGTGTCCTGTGGGTTGGGGTGTGCGGAAGAAAGGG

ACAGAGACTGAGGATGTGCGGTGTAAGCAGTGTGCTCGGGGTACCTTCTCAGATGTGCCTTCTAGTGTGATGAAATGCAA

AGCATACACAGACTGTCTGAGTCAGAACCTGGTGGTGATCAAGCCGGGGACCAAGGAGACAGACAACGTCTGTGGCACAC

TCCCGTCCTTCTCCAGCTCCACCTCACCTTCCCCTGGCACAGCCATCTTTCCACGCCCTGAGCACATGGAAACCCATGAA

GTCCCTTCCTCCACTTATGTTCCCAAAGGCATGAACTCAACAGAATCCAACTCTTCTGCCTCTGTTAGACCAAAGGTACT

GAGTAGCATCCAGGAAGGGACAGTCCCTGACAACACAAGCTCAGCAAGGGGGAAGGAAGACGTGAACAAGACCCTCCCAA

ACCTTCAGGTAGTCAACCACCAGCAAGGCCCCCACCACAGACACATCCTGAAGCTGCTGCCGTCCATGGAGGCCACTGGG

GGCGAGAAGTCCAGCACGCCCATCAAGGGCCCCAAGAGGGGACATCCTAGACAGAACCTACACAAGCATTTTGACATCAA

TGAGCATTTGCCCTGGATGATTGTGCTTTTCCTGCTGCTGGTGCTTGTGGTGATTGTGGTGTGCAGTATCCGGAAAAGCT

CGAGGACTCTGAAAAAGGGGCCCCGGCAGGATCCCAGTGCCATTGTGGAAAAGGCAGGGCTGAAGAAATCCATGACTCCA

ACCCAGAACCGGGAGAAATGGATCTACTACTGCAATGGCCATGGTATCGATATCCTGAAGCTTGTAGCAGCCCAAGTGGG

AAGCCAGTGGAAAGATATCTATCAGTTTCTTTGCAATGCCAGTGAGAGGGAGGTTGCTGCTTTCTCCAATGGGTACACAG

CCGACCACGAGCGGGCCTACGCAGCTCTGCAGCACTGGACCATCCGGGGCCCCGAGGCCAGCCTCGCCCAGCTAATTAGC

GCCCTGCGCCAGCACCGGAGAAACGATGTTGTGGAGAAGATTCGTGGGCTGATGGAAGACACCACCCAGCTGGAAACTGA

CAAACTAGCTCTCCCGATGAGCCCCAGCCCGCTTAGCCCGAGCCCCATCCCCAGCCCCAACGCGAAACTTGAGAATTCCG

CTCTCCTGACGGTGGAGCCTTCCCCACAGGACAAGAACAAGGGCTTCTTCGTGGATGAGTCGGAGCCCCTTCTCCGCTGT

GACTCTACATCCAGCGGCTCCTCCGCGCTGAGCAGGAACGGTTCCTTTATTACCAAAGAAAAGAAGGACACAGTGTTGCG

GCAGGTACGCCTGGACCCCTGTGACTTGCAGCCTATCTTTGATGACATGCTCCACTTTCTAAATCCTGAGGAGCTGCGGG

TGATTGAAGAGATTCCCCAGGCTGAGGACAAACTAGACCGGCTATTCGAAATTATTGGAGTCAAGAGCCAGGAAGCCAGC

CAGACCCTCCTGGACTCTGTTTATAGCCATCTTCCTGACCTGCTGTAG

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

1. FEBS. Lett. 431: 351 (1998). 

2. J. Exp. Med. 194: 1441 (2001). 

3. Oncogene. 20: 7965 (2001). 

4. Immunity 15:23 (2001)

5. J. Immunol. 169: 3993 (2002). 

6. Nature 457:981 (2009).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTP0280-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.