TAJ (Toxicity and JNK inducer), also known as TRADE and TROY, is a single-pass type I membrane glycoprotein in the tumor necrosis factor receptor superfamily (TNFRSF19). TAJ contains 3 TNFR cysteine-rich repeats in the extracellular region but lacks a cytoplasmic death domain (DD). TAJ is widely expressed in the embryo, but shows restricted expression in adult. TAJ is detected in embryonic brain and epithelium, and at lower levels in adult heart, lung and liver. In the adult, TROY is found in the nervous system associated with dorsal root and retinal ganglia neurons, astrocytes, and microglia, and in hair follicles. TAJ has been shown to interact with TRAF family members to activate JNK signaling pathway when overexpressed in cells. TAJ is capable of inducing apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development. TAJ may be a component of the Nogo­A receptor that mediates axon repulsion initiated by one of five myelin­associated inhibitory factors. In the hair follicle, TROY binds Lymphotoxin-α and acts with EDA to drive secondary hair follicle development. TAJ has also been found on melanoma cells, not normal melanocytes.  TAJ has thus been identified as a biomarker for melanoma and represents a promising cell surface target for cell and antibody-based immunotherapies.

TAJ (Toxicity and JNK inducer), also known as TRADE and TROY, is a single-pass type I membrane glycoprotein in the tumor necrosis factor receptor superfamily (TNFRSF19). TAJ contains 3 TNFR cysteine-rich repeats in the extracellular region but lacks a cytoplasmic death domain (DD). TAJ is widely expressed in the embryo, but shows restricted expression in adult. TAJ is detected in embryonic brain and epithelium, and at lower levels in adult heart, lung and liver. In the adult, TROY is found in the nervous system associated with dorsal root and retinal ganglia neurons, astrocytes, and microglia, and in hair follicles. TAJ has been shown to interact with TRAF family members to activate JNK signaling pathway when overexpressed in cells. TAJ is capable of inducing apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development. TAJ may be a component of the Nogo­A receptor that mediates axon repulsion initiated by one of five myelin­associated inhibitory factors. In the hair follicle, TROY binds Lymphotoxin-α and acts with EDA to drive secondary hair follicle development. TAJ has also been found on melanoma cells, not normal melanocytes.  TAJ has thus been identified as a biomarker for melanoma and represents a promising cell surface target for cell and antibody-based immunotherapies.

  

Product Details

 

Gene Symbol: TNFRSF19; TROY; TRADE; TAJ-alpha; UNQ1888/PRO4333

  

NCBI Gene ID: 55504

 

Uniprot Entry: Q9NS68

 

Construct Details: Full length human TAJ gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Sequence:  

ATGGCTTTAAAAGTGCTACTAGAACAAGAGAAAACGTTTTTCACTCTTTTAGTATTACTAGGCTATTTGTCATGTAAAGT

GACTTGTGAATCAGGAGACTGTAGACAGCAAGAATTCAGGGATCGGTCTGGAAACTGTGTTCCCTGCAACCAGTGTGGGC

CAGGCATGGAGTTGTCTAAGGAATGTGGCTTCGGCTATGGGGAGGATGCACAGTGTGTGACGTGCCGGCTGCACAGGTTC

AAGGAGGACTGGGGCTTCCAGAAATGCAAGCCCTGTCTGGACTGCGCAGTGGTGAACCGCTTTCAGAAGGCAAATTGTTC

AGCCACCAGTGATGCCATCTGCGGGGACTGCTTGCCAGGATTTTATAGGAAGACGAAACTTGTCGGCTTTCAAGACATGG

AGTGTGTGCCTTGTGGAGACCCTCCTCCTCCTTACGAACCGCACTGTGCCAGCAAGGTCAACCTCGTGAAGATCGCGTCC

ACGGCCTCCAGCCCACGGGACACGGCGCTGGCTGCCGTTATCTGCAGCGCTCTGGCCACCGTCCTGCTGGCCCTGCTCAT

CCTCTGTGTCATCTATTGTAAGAGACAGTTTATGGAGAAGAAACCCAGCTGGTCTCTGCGGTCACAGGACATTCAGTACA

ACGGCTCTGAGCTGTCGTGTTTTGACAGACCTCAGCTCCACGAATATGCCCACAGAGCCTGCTGCCAGTGCCGCCGTGAC

TCAGTGCAGACCTGCGGGCCGGTGCGCTTGCTCCCATCCATGTGCTGTGAGGAGGCCTGCAGCCCCAACCCGGCGACTCT

TGGTTGTGGGGTGCATTCTGCAGCCAGTCTTCAGGCAAGAAACGCAGGCCCAGCCGGGGAGATGGTGCCGACTTTCTTCG

GATCCCTCACGCAGTCCATCTGTGGCGAGTTTTCAGATGCCTGGCCTCTGATGCAGAATCCCATGGGTGGTGACAACATC

TCTTTTTGTGACTCTTATCCTGAACTCACTGGAGAAGACATTCATTCTCTCAATCCAGAACTTGAAAGCTCAACGTCTTT

GGATTCAAATAGCAGTCAAGATTTGGTTGGTGGGGCTGTTCCAGTCCAGTCTCATTCTGAAAACTTTACAGCAGCTACTG

ATTTATCTAGATATAACAACACACTGGTAGAATCAGCATCAACTCAGGATGCACTAACTATGAGAAGCCAGCTAGATCAG

GAGAGTGGTGCTGTCATCCACCCAGCCACTCAGACGTCCCTCCAGGTAAGGCAGCGACTGGGTTCCCTGTGA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Genomics 62:103 (1999).

2. J. Biol. Chem. 275:15336 (2000).

3. J. Biol. Chem. 275:20742 (2000).

4. Neuron 45:345 (2005).

5. Hum. Mol. Genet. 17:3380 (2008).

 

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTP0291-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.