TACSTD2 (tumor-associated calcium signal transducer 2), also known as Trop2, M1S1, EGP1, GA733-1, gp50 and T16, is a single-pass type I transmembrane glycoprotein of the EPCAM family. It was originally defined by the monoclonal antibody GA733.  TACSTD2 may be formed by the retroposition of the EPCAM gene. TACSTD2 and EPCAM share ~49% amino acid identity and ~67% similarity.  TACSTD2 is highly expressed in a wide variety of epithelial cancers, while little or no expression in adult normal tissue. TACSTD2 is a cell surface receptor that transduces calcium signals and may play a role in tumor growth. TACSTD2 may also have cell adhesive functions. Mutations of the TACSTD2 gene are associated with gelatinous drop-like corneal dystrophy.  TACSTD2 is a target for immunotherapy treatment of human carcinomas.

TACSTD2 (tumor-associated calcium signal transducer 2), also known as Trop2, M1S1, EGP1, GA733-1, gp50 and T16, is a single-pass type I transmembrane glycoprotein of the EPCAM family. It was originally defined by the monoclonal antibody GA733.  TACSTD2 may be formed by the retroposition of the EPCAM gene. TACSTD2 and EPCAM share ~49% amino acid identity and ~67% similarity.  TACSTD2 is highly expressed in a wide variety of epithelial cancers, while little or no expression in adult normal tissue. TACSTD2 is a cell surface receptor that transduces calcium signals and may play a role in tumor growth. TACSTD2 may also have cell adhesive functions. Mutations of the TACSTD2 gene are associated with gelatinous drop-like corneal dystrophy.  TACSTD2 is a target for immunotherapy treatment of human carcinomas.

 

Product Details

 

Gene Symbol: TACSTD2; EGP1; GP50; M1S1; EGP-1; TROP2; GA7331; GA733-1

 

NCBI Gene ID: 4070

 

Uniprot Entry: P09758

 

Construct Details: Full length human TACSTD2 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 972 (bp)

 

Gene Insert Sequence:  

ATGGCTCGGGGCCCCGGCCTCGCGCCGCCACCGCTGCGGCTGCCGCTGCTGCTGCTGGTGCTGGCGGCGG

TGACCGGCCACACGGCCGCGCAGGACAACTGCACGTGTCCCACCAACAAGATGACCGTGTGCAGCCCCGA

CGGCCCCGGCGGCCGCTGCCAGTGCCGCGCGCTGGGCTCGGGCATGGCGGTCGACTGCTCCACGCTGACC

TCCAAGTGTCTGCTGCTCAAGGCGCGCATGAGCGCCCCCAAGAACGCCCGCACGCTGGTGCGGCCGAGTG

AGCACGCGCTCGTGGACAACGATGGCCTCTACGACCCCGACTGCGACCCCGAGGGCCGCTTCAAGGCGCG

CCAGTGCAACCAGACGTCGGTGTGCTGGTGCGTGAACTCGGTGGGCGTGCGCCGCACGGACAAGGGCGAC

CTGAGCCTACGCTGCGATGAGCTGGTGCGCACCCACCACATCCTCATTGACCTGCGCCACCGCCCCACCG

CCGGCGCCTTCAACCACTCAGACCTGGACGCCGAGCTGAGGCGGCTCTTCCGCGAGCGCTATCGGCTGCA

CCCCAAGTTCGTGGCGGCCGTGCACTACGAGCAGCCCACCATCCAGATCGAGCTGCGGCAGAACACGTCT

CAGAAGGCCGCCGGTGACGTGGATATCGGCGATGCCGCCTACTACTTCGAGAGGGACATCAAGGGCGAGT

CTCTATTCCAGGGCCGCGGCGGCCTGGACTTGCGCGTGCGCGGAGAACCCCTGCAGGTGGAGCGCACGCT

CATCTATTACCTGGACGAGATTCCCCCGAAGTTCTCCATGAAGCGCCTCACCGCCGGCCTCATCGCCGTC

ATCGTGGTGGTCGTGGTGGCCCTCGTCGCCGGCATGGCCGTCCTGGTGATCACCAACCGGAGAAAGTCGG

GGAAGTACAAGAAGGTGGAGATCAAGGAACTGGGGGAGTTGAGAAAGGAACCGAGCTTGTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Proc. Natl. Acad. Sci. U.S.A. 86:27-31(1989)

2. Mol. Cell. Biol. 13:1507-1515(1993)

3. Int. J. Cancer 62:610-618(1995)

4. Nat. Genet. 21:420-423(1999)

5. Invest. Ophthalmol. Vis. Sci. 42:2762-2764(2001)

 

 

 

 

Additional supporting documents, including Product Datasheet (PDS), COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.