OSMR (oncostatin M receptor), also known as OSMRB (OSMR subunit beta) and IL31RB (IL-31 receptor subunit beta), is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 2 subfamily.  OSMR consists of an extracellular region with 4 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a cytoplasmic tail with a box 1 motif.  The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding, while the box 1 motif is required for JAK interaction and activation.  OSMR heterodimerizes with IL6ST (interleukin 6 signal transducer) or gp130 to form the type II OSMR complex and with IL31RA to form the IL31R complex, and thus transduces oncostatin M and IL31 induced signaling events.  Mutations in the OSMR gene have been associated with familial primary localized cutaneous amyloidosis (PLCA1). 

OSMR (oncostatin M receptor), also known as OSMRB (OSMR subunit beta) and IL31RB (IL-31 receptor subunit beta), is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 2 subfamily.  OSMR consists of an extracellular region with 4 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a cytoplasmic tail with a box 1 motif.  The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding, while the box 1 motif is required for JAK interaction and activation.  OSMR heterodimerizes with IL6ST (interleukin 6 signal transducer) or gp130 to form the type II OSMR complex and with IL31RA to form the IL31R complex, and thus transduces oncostatin M and IL31 induced signaling events.  Mutations in the OSMR gene have been associated with familial primary localized cutaneous amyloidosis (PLCA1). 

 

Product Details

 

Gene Symbol: OSMR; OSMRB; IL31RB; PLCA1; IL-31RB; IL-31R-beta

 

NCBI Gene ID: 9180

 

Uniprot Entry: Q99650

 

Construct Details: The recombinant human OSMR-Fc protein is expressed as a 939 amino acid protein consisting of Glu28 - Ser739 region of OSMR with a Fc-fusion from human IgG1, which exists as a dimer under non-reducing conditions.  

   

Source: Human cells stably expressing OSMR-Fc protein and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60
ERLPLTPVSL KVSTNSTRQS LHLQWTVHNL PYHQELKMVF QIQISRIETS NVIWVGNYST

        70         80         90        100        110        120
TVKWNQVLHW SWESELPLEC ATHFVRIKSL VDDAKFPEPN FWSNWSSWEE VSVQDSTGQD

       130        140        150        160        170        180
ILFVFPKDKL VEEGTNVTIC YVSRNIQNNV SCYLEGKQIH GEQLDPHVTA FNLNSVPFIR

       190        200        210        220        230        240
NKGTNIYCEA SQGNVSEGMK GIVLFVSKVL EEPKDFSCET EDFKTLHCTW DPGTDTALGW

       250        260        270        280        290        300
SKQPSQSYTL FESFSGEKKL CTHKNWCNWQ ITQDSQETYN FTLIAENYLR KRSVNILFNL

       310        320        330        340        350        360
THRVYLMNPF SVNFENVNAT NAIMTWKVHS IRNNFTYLCQ IELHGEGKMM QYNVSIKVNG

       370        380        390        400        410        420
EYFLSELEPA TEYMARVRCA DASHFWKWSE WSGQNFTTLE AAPSEAPDVW RIVSLEPGNH

       430        440        450        460        470        480
TVTLFWKPLS KLHANGKILF YNVVVENLDK PSSSELHSIP APANSTKLIL DRCSYQICVI

       490        500        510        520        530        540
ANNSVGASPA SVIVISADPE NKEVEEERIA GTEGGFSLSW KPQPGDVIGY VVDWCDHTQD

       550        560        570        580        590        600
VLGDFQWKNV GPNTTSTVIS TDAFRPGVRY DFRIYGLSTK RIACLLEKKT GYSQELAPSD

       610        620        630        640        650        660
NPHVLVDTLT SHSFTLSWKD YSTESQPGFI QGYHVYLKSK ARQCHPRFEK AVLSDGSECC

       670        680        690        700        710        720
KYKIDNPEEK ALIVDNLKPE SFYEFFITPF TSAGEGPSAT FTKVTTPDEH SSTGTHTCPP

       730        740        750        760        770        780
CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK

       790        800        810        820        830        840
TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV

       850        860        870        880        890        900
YTLPPSREEM TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS

       910        920        930
KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK

 

 

M.W.: Calculated molecular mass 106.5 kDa; estimated by SDS-PAGE under reducing condition 120-130 kDa with a minor band at ~90 kDa probably due to proteolytic cleavage

 

Calculated PI: 5.84

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 193545

  

Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

References

  

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.