CD116, also known as GM-CSFRα (granulocyte macrophage colony stimulating factor receptor alpha), is a component of the receptor complex that mediates cellular responses to GM­CSF. This receptor complex is composed of a cytokine-specific alpha- chain CD116 and a beta-chain CD131 shared with the receptors for IL-3 and IL-5. CD116 alone binds GM-CSF with low affinity, whereas CD131 does not bind GM-CSF by itself. Association with CD131 converts CD116 from a low affinity to a high affinity receptor for GM­CSF. GM­CSF is a hematopoietic cytokine that regulates differentiation, survival, and proliferation of colony-forming unit-granulocyte-macrophage progenitor cells. It promotes the differentiation and mobilization of granulocyte­macrophage, erythroid, megakaryocyte, and eosinophil progenitors. GM­CSF also enhances the activation of myeloid cell effector functions and plays an important role in the development of Th1 biased immune responses, allergic inflammation, and autoimmunity. CD116 is a single pass, type I transmembrane glycoprotein that consists of a 298 amino acid (aa) extracellular region with 2 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a 54 aa cytoplasmic tail that mediates the activation of Janus kinase (Jak)- Stat and MAPK as well as phosphatidylinositol 3-kinase (PI3K) pathways.  CD116 is expressed on hematopoietic stem cells, progenitor and differentiated cells in the myeloid lineage, vascular endothelial cells, and placenta as well as in those non­hematopoietic tumor cells.  Soluble forms of CD116 retain the ability to bind GM­CSF. 

CD116, also known as GM-CSFRα (granulocyte macrophage colony stimulating factor receptor alpha), is a component of the receptor complex that mediates cellular responses to GM­CSF. This receptor complex is composed of a cytokine-specific alpha- chain CD116 and a beta-chain CD131 shared with the receptors for IL-3 and IL-5. CD116 alone binds GM-CSF with low affinity, whereas CD131 does not bind GM-CSF by itself. Association with CD131 converts CD116 from a low affinity to a high affinity receptor for GM­CSF. GM­CSF is a hematopoietic cytokine that regulates differentiation, survival, and proliferation of colony-forming unit-granulocyte-macrophage progenitor cells. It promotes the differentiation and mobilization of granulocyte­macrophage, erythroid, megakaryocyte, and eosinophil progenitors. GM­CSF also enhances the activation of myeloid cell effector functions and plays an important role in the development of Th1 biased immune responses, allergic inflammation, and autoimmunity. CD116 is a single pass, type I transmembrane glycoprotein that consists of a 298 amino acid (aa) extracellular region with 2 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a 54 aa cytoplasmic tail that mediates the activation of Janus kinase (Jak)- Stat and MAPK as well as phosphatidylinositol 3-kinase (PI3K) pathways.  CD116 is expressed on hematopoietic stem cells, progenitor and differentiated cells in the myeloid lineage, vascular endothelial cells, and placenta as well as in those non­hematopoietic tumor cells.  Soluble forms of CD116 retain the ability to bind GM­CSF. 

 

Product Details

 

Gene Symbol: CD116; GMR; CSF2R; CSF2RA; SMDP4; CDw116; CSF2RX; CSF2RY; GMCSFR; CSF2RAX; CSF2RAY; GM-CSF-R-alpha; GMR-alpha

 

NCBI Gene ID: 1438

 

Uniprot Entry: P15509

 

Construct Details: The recombinant human CD116-Fc fusion is expressed as a 536 amino acid protein consisting of Asp23 - Gly320 region of CD116 (Uniprot Accession #P15509) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source:  Human cells stably expressing CD116-Fc growing in chemical-defined media with no animal components or antibiotics 

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

EKSDLRTVAP ASSLNVRFDS RTMNLSWDCQ ENTTFSKCFL TDKKNRVVEP RLSNNECSCT 

        70         80         90        100        110        120 

FREICLHEGV TFEVHVNTSQ RGFQQKLLYP NSGREGTAAQ NFSCFIYNAD LMNCTWARGP  

       130        140        150        160        170        180 

TAPRDVQYFL YIRNSKRRRE IRCPYYIQDS GTHVGCHLDN LSGLTSRNYF LVNGTSREIG 

       190        200        210        220        230        240 

IQFFDSLLDT KKIERFNPPS NVTVRCNTTH CLVRWKQPRT YQKLSYLDFQ YQLDVHRKNT 

       250        260        270        280        290        300 

QPGTENLLIN VSGDLENRYN FPSSEPRAKH SVKIRAADVR ILNWSSWSEA IEFGSDDGST 

       310        320        330        340        350        360 

TENLYFQGST GTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP  

       370        380        390        400        410        420 

EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP 

       430        440        450        460        470        480 

IEKTISKAKG QPREPQVYTL PPSREEMTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY 

       490        500        510        520        530 

KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK 

 

M.W.: Calculated molecular mass (kDa): 61.2; Estimated by SDS-PAGE under reducing condition (kDa): 75-80

 

Calculated PI: 8.45

 

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm): 81790

 

Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Inhibits GM­CSF­dependent proliferation of human erythroleukemic TF-1 cells. 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C for immediate use. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. EMBO J. 8:3667 (1989) 

2. Proc. Natl. Acad. Sci. 87:9655 (1990)

3. Proc. Natl. Acad. Sci. 88: 5082 (1991)

4. J. Biol. Chem. 268:11881 (1993).

5. Crit. Rev. Immunol. 25:405 (2005)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2268B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.