CLEC4M (C-type lectin domain family 4 member C) or CD299, is a single-pass type II transmembrane protein that belongs to a the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. CLEC4M is often referred to as L-SIGN because of its expression in the endothelial cells of the lymph nodes and liver. CLEC4M is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses. CLEC4M contains 1 tandem-repeat neck domain and 1 C-type lectin domain in the extracellular region with a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells.  The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity.  CLEC4M is closely related in terms of both sequence and function to a neighboring gene DC-SIGN or CD209. They differ in their ligand-binding properties and distribution. CLEC4M may act as a pathogen-recognition receptor involved in peripheral immune surveillance in liver. CLEC4M may mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. It is also a receptor for ICAM3, probably by binding to mannose-like carbohydrates.CLEC4M acts as an attachment receptor for ebola virus, hepatitis C virus, HIV-1 ,  coronavirus 229E, cytomegalovirus/HHV-5, influenzavirus, SARS coronavirus, and west-nile virus etc.

CLEC4M (C-type lectin domain family 4 member C) or CD299, is a single-pass type II transmembrane protein that belongs to a the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. CLEC4M is often referred to as L-SIGN because of its expression in the endothelial cells of the lymph nodes and liver. CLEC4M is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses. CLEC4M contains 1 tandem-repeat neck domain and 1 C-type lectin domain in the extracellular region with a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells.  The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity.  CLEC4M is closely related in terms of both sequence and function to a neighboring gene DC-SIGN or CD209. They differ in their ligand-binding properties and distribution. CLEC4M may act as a pathogen-recognition receptor involved in peripheral immune surveillance in liver. CLEC4M may mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. It is also a receptor for ICAM3, probably by binding to mannose-like carbohydrates.CLEC4M acts as an attachment receptor for ebola virus, hepatitis C virus, HIV-1 ,  coronavirus 229E, cytomegalovirus/HHV-5, influenzavirus, SARS coronavirus, and west-nile virus etc.

 

Product Details

 

Gene Symbol: CLEC4M; CD299; LSIGN; CD209L; L-SIGN; DCSIGNR; HP10347; DC-SIGN2; DC-SIGNR

 

NCBI Gene ID: 10332

 

Uniprot Entry: Q9H2X3

 

Construct Details: The recombinant human CLEC4M-Fc is expressed as a 557-amino acid protein consisting of Gln71 -  Glu399 region of CLEC4M and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.  

   

Source: Human cells stably expressing CLEC4M-Fc protein and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60
QVSKVPSSLS QEQSEQDAIY QNLTQLKAAV GELSEKSKLQ EIYQELTQLK AAVGELPEKS

        70         80         90        100        110        120
KLQEIYQELT RLKAAVGELP EKSKLQEIYQ ELTRLKAAVG ELPEKSKLQE IYQELTRLKA

       130        140        150        160        170        180
AVGELPEKSK LQEIYQELTE LKAAVGELPE KSKLQEIYQE LTQLKAAVGE LPDQSKQQQI

       190        200        210        220        230        240
YQELTDLKTA FERLCRHCPK DWTFFQGNCY FMSNSQRNWH DSVTACQEVR AQLVVIKTAE

       250        260        270        280        290        300
EQNFLQLQTS RSNRFSWMGL SDLNQEGTWQ WVDGSPLSPS FQRYWNSGEP NNSGNEDCAE

       310        320        330        340        350        360
FSGSGWNDNR CDVDNYWICK KPAACFRDES TGTHTCPPCP APELLGGPSV FLFPPKPKDT

       370        380        390        400        410        420
LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH

       430        440        450        460        470        480
QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSREEMTK NQVSLTCLVK

       490        500        510        520        530        540
GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE

       550
ALHNHYTQKS LSLSPGK

 

 

M.W.: Calculated molecular mass 63.3 kDa; estimated by SDS-PAGE under reducing condition 65-70 kDa

 

Calculated PI: 5.49

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 96675

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.