CD207 or CLEC4K (C-type lectin domain family 4 member K) is a single-pass type II transmembrane protein and a member of the C-type lectin superfamily.  CLEC4K/CD207 contains 1 C-type lectin domain in the extracellular region. CLEC4K is expressed only in Langerhans cells which are immature dendritic cells of the epidermis and mucosa. CLEC4K is localized in the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed and zippered membranes. CLEC4K is a C-type lectin with mannose binding specificity, and it has been proposed that mannose binding by this protein leads to internalization of antigen into Birbeck granules and providing access to a nonclassical antigen-processing pathway. Mutations in the CLEC4K gene result in Birbeck granules deficiency or loss of sugar binding activity. CLEC4K binds to sulfated as well as mannosylated glycans, keratan sulfate (KS) and beta-glucans. It facilitates uptake of antigens and is involved in the routing and/or processing of antigen for presentation to T cells. CLEC4K acts as a major receptor on primary Langerhans cells for Candida species, Saccharomyces species, and Malassezia furfur. CLEC4K protects against human immunodeficiency virus-1 (HIV-1) infection. CLEC4K binds to high-mannose structures present on the envelope glycoprotein followed by subsequent targeting of the virus to the Birbeck granules leading to its rapid degradation.

CD207 or CLEC4K (C-type lectin domain family 4 member K) is a single-pass type II transmembrane protein and a member of the C-type lectin superfamily.  CLEC4K/CD207 contains 1 C-type lectin domain in the extracellular region. CLEC4K is expressed only in Langerhans cells which are immature dendritic cells of the epidermis and mucosa. CLEC4K is localized in the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed and zippered membranes. CLEC4K is a C-type lectin with mannose binding specificity, and it has been proposed that mannose binding by this protein leads to internalization of antigen into Birbeck granules and providing access to a nonclassical antigen-processing pathway. Mutations in the CLEC4K gene result in Birbeck granules deficiency or loss of sugar binding activity. CLEC4K binds to sulfated as well as mannosylated glycans, keratan sulfate (KS) and beta-glucans. It facilitates uptake of antigens and is involved in the routing and/or processing of antigen for presentation to T cells. CLEC4K acts as a major receptor on primary Langerhans cells for Candida species, Saccharomyces species, and Malassezia furfur. CLEC4K protects against human immunodeficiency virus-1 (HIV-1) infection. CLEC4K binds to high-mannose structures present on the envelope glycoprotein followed by subsequent targeting of the virus to the Birbeck granules leading to its rapid degradation.

 

Product Details

 

Gene Symbol: CLEC4K; CD207

 

NCBI Gene ID: 50489

 

Uniprot Entry: O9UJ71

 

Construct Details: Full length human CD207 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 987 (bp)

 

Gene Insert Sequence:  

ATGACTGTGGAGAAGGAGGCCCCTGATGCGCACTTCACTGTGGACAAACAGAACATCTCCCTCTGGCCCC

GAGAGCCTCCTCCCAAGTCCGGTCCATCTCTGGTCCCGGGGAAAACACCCACAGTCCGTGCTGCATTAAT

CTGCCTGACGCTGGTCCTGGTCGCCTCCGTCCTGCTGCAGGCCGTCCTTTATCCCCGGTTTATGGGCACC

ATATCAGATGTAAAGACCAATGTCCAGTTGCTGAAAGGTCGTGTGGACAACATCAGCACCCTGGATTCTG

AAATTAAAAAGAATAGTGACGGCATGGAGGCAGCTGGCGTTCAGATCCAGATGGTGAATGAGAGCCTGGG

TTATGTGCGTTCTCAGTTCCTGAAGTTAAAAACCAGTGTGGAGAAGGCCAACGCACAGATCCAGATCTTA

ACAAGAAGTTGGGAAGAAGTCAGTACCTTAAATGCCCAAATCCCAGAGTTAAAAAGTGATTTGGAGAAAG

CCAGTGCTTTAAATACAAAGATCCGGGCACTCCAGGGCAGCTTGGAGAATATGAGCAAGTTGCTCAAACG

ACAAAATGATATTCTACAGGTGGTTTCTCAAGGCTGGAAGTACTTCAAGGGGAACTTCTATTACTTTTCT

CTCATTCCAAAGACCTGGTATAGTGCCGAGCAGTTCTGTGTGTCCAGGAATTCACACCTGACCTCGGTGA

CCTCAGAGAGTGAGCAGGAGTTTCTGTATAAAACAGCGGGGGGACTCATCTACTGGATTGGCCTGACTAA

AGCAGGGATGGAAGGGGACTGGTCCTGGGTGGATGACACGCCATTCAACAAGGTCCAAAGTGTGAGGTTC

TGGATTCCAGGTGAGCCCAACAATGCTGGGAACAATGAACACTGTGGCAATATAAAGGCTCCCTCACTTC

AGGCCTGGAATGATGCCCCATGTGACAAAACGTTTCTTTTCATTTGTAAGCGACCCTATGTCCCATCAGA

ACCGTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.