The TGFβ (transforming growth factor β) superfamily proteins are pleiotropic cytokines that regulate a diverse range of cellular processes, including proliferation, differentiation, migration, adhesion and death.  The TGFβ superfamily elicits 4 signaling pathways: TGFβ, Bone Morphogenic Protein (BMP), Activin, and Nodal.  Each pathway signals through a heteromeric receptor complex composed of at least two type-I and two type-II transmembrane receptors containing cytoplasmic serine/threonine kinase domains. These receptors are distinguished by the presence of a glycine/serine-rich juxta-membrane domain found only in the type I receptors. Either type receptor may initially bind ligand, followed by the recruitment of an alternate-type receptor counterpart to form a signal-activating complex. AMHR2 (Anti-Müllerian hormone type 2 receptor), also known as MRII and MISRII, is a type II receptor that forms a heteromeric complex with various type I TGFBRs to mediate AMH (anti-Müllerian hormone) or MIS (Müllerian inhibiting substance) signaling. AMH/MIS, in addition to testosterone, plays a critical role in male sex differentiation. AMH and testosterone are produced in the testes by different cells and show different effects. Testosterone promotes the development of male genitalia, while the binding of AMH/MIS to AMHR2 prevents the development of the mullerian ducts into uterus and Fallopian tubes. Mutations in the AMHR2 are associated with persistent Müllerian duct syndrome type 2 (PMDS2). AMHR2 may suppress tumorigenesis in testes. 

The TGFβ (transforming growth factor β) superfamily proteins are pleiotropic cytokines that regulate a diverse range of cellular processes, including proliferation, differentiation, migration, adhesion and death.  The TGFβ superfamily elicits 4 signaling pathways: TGFβ, Bone Morphogenic Protein (BMP), Activin, and Nodal.  Each pathway signals through a heteromeric receptor complex composed of at least two type-I and two type-II transmembrane receptors containing cytoplasmic serine/threonine kinase domains. These receptors are distinguished by the presence of a glycine/serine-rich juxta-membrane domain found only in the type I receptors. Either type receptor may initially bind ligand, followed by the recruitment of an alternate-type receptor counterpart to form a signal-activating complex. AMHR2 (Anti-Müllerian hormone type 2 receptor), also known as MRII and MISRII, is a type II receptor that forms a heteromeric complex with various type I TGFBRs to mediate AMH (anti-Müllerian hormone) or MIS (Müllerian inhibiting substance) signaling. AMH/MIS, in addition to testosterone, plays a critical role in male sex differentiation. AMH and testosterone are produced in the testes by different cells and show different effects. Testosterone promotes the development of male genitalia, while the binding of AMH/MIS to AMHR2 prevents the development of the mullerian ducts into uterus and Fallopian tubes. Mutations in the AMHR2 are associated with persistent Müllerian duct syndrome type 2 (PMDS2). AMHR2 may suppress tumorigenesis in testes. 

 

Product Details

 

Gene Symbol: AMHR2; AMHR; MRII; MISR2; MISRII; MR2

 

NCBI Gene ID: 269

 

Uniprot Entry: Q16671

 

Construct Details: The recombinant human AMHR2-Fc fusion protein is expressed as a 354 amino acid protein consisting of Pro18 - Ser144 region of AMHR2 (UniProt accession #Q16671) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition (see the gel image above, labeled as "DTT: -").

 

Source: Human cells stably expressing human AMHR2-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence:

PPNRRTCVFFEAPGVRGSTKTLGELLDTGTELPRAIRCLYSRCCFGIWNLTQDRAQ

VEMQGCRDSDEPGCESLHCDPSPRAHPSPGSTLFTCSCGTDFCNANYSHLPPPGSP

GTPGSQGPQAAPGESTGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTC

VVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGK

EYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFY

PSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMH

EALHNHYTQKSLSLSPGK

 

M.W.: Calculated molecular mass (kDa): 39.0; Estimated by SDS-PAGE under reducing condition (kDa): 50-55

 

Calculated PI: 6.40

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  44890

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Recombinant AMHR2 protein binds human AMH/MIS and blocks AMH/MIS-induced signaling activity

 

 

 

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Development 120:189 (1994).  

2. Nat. Genet. 11:382 (1995).  

3. Endocrinology 137:160 (1996).  

4. Hum. Mol. Genet. 5:1269 (1996). 

5. Carcinogenesis. 33:2351(2012).  

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL0356-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.