The TGFβ (transforming growth factor β) superfamily proteins are pleiotropic cytokines that regulate a diverse range of cellular processes, including proliferation, differentiation, migration, adhesion and death.  The TGFβ superfamily elicits 4 signaling pathways: TGFβ, Bone Morphogenic Protein (BMP), Activin, and Nodal.  Each pathway signals through a heteromeric receptor complex composed of at least two type-I and two type-II transmembrane receptors containing cytoplasmic serine/threonine kinase domains. These receptors are distinguished by the presence of a glycine/serine-rich juxta-membrane domain found only in the type I receptors. Either type receptor may initially bind ligand, followed by the recruitment of an alternate-type receptor counterpart to form a signal-activating complex. ACVR2B (Activin receptor type-2B), also known as ACTR-IIB, is a type-II receptors for Activin  and Myostatin. Ligands such as Activin and Myostatin bind to ACVR2B, which then associates with a type I receptor to initiate signal transduction. Human, mouse and rat ACVRIIB share greater than 98% amino acid sequence homology. Recombinant soluble ACVRIIB bind Activin with high affinity, and are potent Activin antagonists. Myostatin, a negative regulator of skeletal muscle growth, binds to ACVR2B effectively, and to a lesser extent, to ACVR2A. Haplotype structure at the ACVR2B gene locus may contribute to interindividual variation in skeletal muscle mass and strength. A heterozygous mutation in ACVR2B is a cause of left-right axis malformation, visceral heterotaxy-4 (HTX4). 

The TGFβ (transforming growth factor β) superfamily proteins are pleiotropic cytokines that regulate a diverse range of cellular processes, including proliferation, differentiation, migration, adhesion and death.  The TGFβ superfamily elicits 4 signaling pathways: TGFβ, Bone Morphogenic Protein (BMP), Activin, and Nodal.  Each pathway signals through a heteromeric receptor complex composed of at least two type-I and two type-II transmembrane receptors containing cytoplasmic serine/threonine kinase domains. These receptors are distinguished by the presence of a glycine/serine-rich juxta-membrane domain found only in the type I receptors. Either type receptor may initially bind ligand, followed by the recruitment of an alternate-type receptor counterpart to form a signal-activating complex. ACVR2B (Activin receptor type-2B), also known as ACTR-IIB, is a type-II receptors for Activin  and Myostatin. Ligands such as Activin and Myostatin bind to ACVR2B, which then associates with a type I receptor to initiate signal transduction. Human, mouse and rat ACVRIIB share greater than 98% amino acid sequence homology. Recombinant soluble ACVRIIB bind Activin with high affinity, and are potent Activin antagonists. Myostatin, a negative regulator of skeletal muscle growth, binds to ACVR2B effectively, and to a lesser extent, to ACVR2A. Haplotype structure at the ACVR2B gene locus may contribute to interindividual variation in skeletal muscle mass and strength. A heterozygous mutation in ACVR2B is a cause of left-right axis malformation, visceral heterotaxy-4 (HTX4). 

 

Product Details

 

Gene Symbol: ACVR2B; HTX4; ACTRIIB; ActR-IIB; ACVRIIB; ACVR-IIB

 

NCBI Gene ID: 93

 

Uniprot Entry: Q13705

 

Construct Details: The recombinant human ACVR2B-Fc fusion protein is expressed as a 344 amino acid protein consisting of Ser19 - Thr134 region of ACVR2B (UniProt accession #Q13705) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition (see the gel image above, labeled as "DTT: -")

 

Source: Human cells stably expressing human ACVR2B-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence:

SGRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWRN

SSGTIELVKKGCWLDDFNCYDRQECVATEENPQVYFCCCEGNFCNER

FTHLPEAGGPEVTYEPPPTAPTSTGTHTCPPCPAPELLGGPSVFLFP

PKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKP

REEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISK

AKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNG

QPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEAL

HNHYTQKSLSLSPGK

 

 

M.W.: Calculated molecular mass (kDa): 38.9; Estimated by SDS-PAGE under reducing condition (kDa): ~55

 

Calculated PI: 5.49

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  61850

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Immobilized ACVR2B binds Activin or Inhibin in a functional ELISA.  Neutralize Activin-induced inhibition of MPC11 cell proliferation and hemoglobin expression in K562 human chronic myelogenous leukemia cells.

 

 

 

 

 

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Blood 83:2163(1994). 

2. Mol. Cell. Biol. 16:1066(1996). 

3. Nature Genet. 17: 252(1997).

4. Genes Dev. 11: 1812(1997). 

5. Am. J. Med. Genet. 82: 70(1999). 

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL0138-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.