CD87, also known as u-PAR/UPAR (Urokinase plasminogen activator surface receptor) and PLAUR (plasminogen activator, urokinase receptor), is a glycosyl-phosphatidylinositol (GPI)-linked cell surface or secreted glycoprotein. A variety of GPI-linked surface glycoproteins are composed of one or more copies of a conserved domain of ~100 amino-acid residues termed u-PAR/Ly-6 motif that was originally found in CD87/u-PAR and Ly-6/CD59. The u-PAR/Ly-6 (UL) motif has 10 conserved cysteines with a defined disulfide-bonding pattern. CD87 contains 3 copies of u-PAR/Ly6 motifs in the extracellular domain (ECD).  CD87 is the receptor for UPA (urokinase plasminogen activator) and influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix.  CD87 binds both the pro-protein and mature forms of UPA and permits the activation of the receptor-bound pro-enzyme by plasmin. CD87 thus plays a role in localizing and promoting plasmin formation. CD87 also mediates the proteolysis-independent signal transduction activation effects of U-PA. CD87 is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.

CD87, also known as u-PAR/UPAR (Urokinase plasminogen activator surface receptor) and PLAUR (plasminogen activator, urokinase receptor), is a glycosyl-phosphatidylinositol (GPI)-linked cell surface or secreted glycoprotein. A variety of GPI-linked surface glycoproteins are composed of one or more copies of a conserved domain of ~100 amino-acid residues termed u-PAR/Ly-6 motif that was originally found in CD87/u-PAR and Ly-6/CD59. The u-PAR/Ly-6 (UL) motif has 10 conserved cysteines with a defined disulfide-bonding pattern. CD87 contains 3 copies of u-PAR/Ly6 motifs in the extracellular domain (ECD).  CD87 is the receptor for UPA (urokinase plasminogen activator) and influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix.  CD87 binds both the pro-protein and mature forms of UPA and permits the activation of the receptor-bound pro-enzyme by plasmin. CD87 thus plays a role in localizing and promoting plasmin formation. CD87 also mediates the proteolysis-independent signal transduction activation effects of U-PA. CD87 is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.

 

Product Details

 

Gene Symbol: CD87; UPAR; PLAUR; URKR; U-PAR; MO3

 

NCBI Gene ID: 5329

 

Uniprot Entry: Q03405

 

Construct Details: The recombinant human CD87-Fc fusion is expressed as a 509 amino acid protein consisting of Leu23 - Ser304 region of CD87 and a C-terminal Fc from human IgG1.

   

Source: Human cells stably expressing CD87-Fc and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60
LRCMQCKTNG DCRVEECALG QDLCRTTIVR LWEEGEELEL VEKSCTHSEK TNRTLSYRTG

        70         80         90        100        110        120
LKITSLTEVV CGLDLCNQGN SGRAVTYSRS RYLECISCGS SDMSCERGRH QSLQCRSPEE

       130        140        150        160        170        180
QCLDVVTHWI QEGEEGRPKD DRHLRGCGYL PGCPGSNGFH NNDTFHFLKC CNTTKCNEGP

       190        200        210        220        230        240
ILELENLPQN GRQCYSCKGN STHGCSSEET FLIDCRGPMN QCLVATGTHE PKNQSYMVRG

       250        260        270        280        290        300
CATASMCQHA HLGDAFSMNH IDVSCCTKSG CNHPDLDVQY RSTGTHTCPP CPAPELLGGP

       310        320        330        340        350        360
SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS

       370        380        390        400        410        420
TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSREEM

       430        440        450        460        470        480
TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ

       490        500
QGNVFSCSVM HEALHNHYTQ KSLSLSPGK  

 

M.W.: Calculated molecular mass 56.9 kDa; estimated by SDS-PAGE under reducing condition: ~55-65 kDa 

 

Calculated PI: 6.27

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  58965

  

Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining).  The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.