CD177 is a glycosyl-phosphatidylinositol (GPI)-anchored cell surface glycoprotein that plays a role in neutrophil activation. A variety of GPI-linked surface glycoproteins are composed of one or more copies of a conserved domain of ~100 amino-acid residues termed UPAR/Ly6 motif that was originally found in UPAR (Urokinase plasminogen activator surface receptor) and Ly6/CD59. The u-PAR/Ly-6 (UL) motif has 10 conserved cysteines with a defined disulfide-bonding pattern.  CD177 contains 2 copies of  UPAR/Ly6 motifs in the extracellular domain (ECD).  CD177 is highly expressed in normal bone marrow and weakly expressed in fetal liver. CD177 is detected  on neutrophils and in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET). A soluble form of CD177 may be produced by proteolytic cleavage at the cell surface (shedding).  CD177 binds platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in the CD177 gene are associated with myeloproliferative diseases. Over-expression of the CD177 gene has been found in patients with polycythemia rubra vera. Autoantibodies against CD177 may result in pulmonary transfusion reactions, and CD177 may be involved in Wegener's granulomatosis.

CD177 is a glycosyl-phosphatidylinositol (GPI)-anchored cell surface glycoprotein that plays a role in neutrophil activation. A variety of GPI-linked surface glycoproteins are composed of one or more copies of a conserved domain of ~100 amino-acid residues termed UPAR/Ly6 motif that was originally found in UPAR (Urokinase plasminogen activator surface receptor) and Ly6/CD59. The u-PAR/Ly-6 (UL) motif has 10 conserved cysteines with a defined disulfide-bonding pattern.  CD177 contains 2 copies of  UPAR/Ly6 motifs in the extracellular domain (ECD).  CD177 is highly expressed in normal bone marrow and weakly expressed in fetal liver. CD177 is detected  on neutrophils and in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET). A soluble form of CD177 may be produced by proteolytic cleavage at the cell surface (shedding).  CD177 binds platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in the CD177 gene are associated with myeloproliferative diseases. Over-expression of the CD177 gene has been found in patients with polycythemia rubra vera. Autoantibodies against CD177 may result in pulmonary transfusion reactions, and CD177 may be involved in Wegener's granulomatosis.

 

Product Details

 

Gene Symbol: CD177; NB1; PRV1; HNA2A; PRV-1; HNA-2a; NB1GP

 

NCBI Gene ID: 57126

 

Uniprot Entry: Q8N6Q3

 

Construct Details: Full length human CD177 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1314 (bp)

 

Gene Insert Sequence:  

ATGAGCGCGGTATTACTGCTGGCCCTCCTGGGGTTCATCCTCCCACTGCCAGGAGTGCAGGCGCTGCTCT

GCCAGTTTGGGACAGTTCAGCATGTGTGGAAGGTGTCCGACCTGCCCCGGCAATGGACCCCTAAGAACAC

CAGCTGCGACAGCGGCTTGGGGTGCCAGGACACGTTGATGCTCATTGAGAGCGGACCCCAAGTGAGCCTG

GTGCTCTCCAAGGGCTGCACGGAGGCCAAGGACCAGGAGCCCCGCGTCACTGAGCACCGGATGGGCCCCG

GCCTCTCCCTGATCTCCTACACCTTCGTGTGCCGCCAGGAGGACTTCTGCAACAACCTCGTTAACTCCCT

CCCGCTTTGGGCCCCACAGCCCCCAGCAGACCCAGGATCCTTGAGGTGCCCAGTCTGCTTGTCTATGGAA

GGCTGTCTGGAGGGGACAACAGAAGAGATCTGCCCCAAGGGGACCACACACTGTTATGATGGCCTCCTCA

GGCTCAGGGGAGGAGGCATCTTCTCCAATCTGAGAGTCCAGGGATGCATGCCCCAGCCAGTTTGCAACCT

GCTCAATGGGACACAGGAAATTGGGCCCGTGGGTATGACTGAGAACTGCGATATGAAAGATTTTCTGACC

TGTCATCGGGGGACCACCATTATGACACACGGAAACTTGGCTCAAGAACCCACTGATTGGACCACATCGA

ATACCGAGATGTGCGAGGTGGGGCAGGTGTGTCAGGAGACGCTGCTGCTCCTAGATGTAGGACTCACATC

AACCCTGGTGGGGACAAAAGGCTGCAGCACTGTTGGGGCTCAAAATTCCCAGAAGACCACCATCCACTCA

GCCCCTCCTGGGGTGCTTGTGGCCTCCTATACCCACTTCTGCTCCTCGGACCTGTGCAATAGTGCCAGCA

GCAGCAGCGTTCTGCTGAACTCCCTCCCTCCTCAAGCTGCCCCTGTCCCAGGAGACCGGCAGTGTCCTAC

CTGTGTGCAGCCCCTTGGAACCTGTTCAAGTGGCTCCCCCCGAATGACCTGCCCCAGGGGCGCCACTCAT

TGTTATGATGGGTACATTCATCTCTCAGGAGGTGGGCTGTCCACCAAAATGAGCATTCAGGGCTGCGTGG

CCCAACCTTCCAGCTTCTTGTTGAACCACACCAGACAAATCGGGATCTTCTCTGCGCGTGAGAAGCGTGA

TGTGCAGCCTCCTGCCTCTCAGCATGAGGGAGGTGGGGCTGAGGGCCTGGAGTCTCTCACTTGGGGGGTG

GGGCTGGCACTGGCCCCAGCGCTGTGGTGGGGAGTGGTTTGCCCTTCCTGCTAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.