CD125, also known as IL5RA/IL5R (Interleukin-5 receptor subunit alpha), is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 5 subfamily.  CD125/IL5RA consists of an extracellular region with 2 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a cytoplasmic tail with the box 1 motif.  The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding, while the box 1 motif is required for JAK interaction/activation. CD125/IL5RA is expressed on eosinophils and basophils. CD125/IL5RA is a component of the receptor complex specific for interleukin-5. The IL5 receptor complex is a heterodimer of CD125/IL5RA and the common signal transducing subunit CD131/CSF2RB shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of CD125 to IL5 depends on the beta subunit CD131. The beta subunit CD131 is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4.

CD125, also known as IL5RA/IL5R (Interleukin-5 receptor subunit alpha), is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 5 subfamily.  CD125/IL5RA consists of an extracellular region with 2 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a cytoplasmic tail with the box 1 motif.  The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding, while the box 1 motif is required for JAK interaction/activation. CD125/IL5RA is expressed on eosinophils and basophils. CD125/IL5RA is a component of the receptor complex specific for interleukin-5. The IL5 receptor complex is a heterodimer of CD125/IL5RA and the common signal transducing subunit CD131/CSF2RB shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of CD125 to IL5 depends on the beta subunit CD131. The beta subunit CD131 is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4.

 

Product Details

 

Gene Symbol: IL5RA; IL5R; CD125; CDw125; HSIL5R3

 

NCBI Gene ID: 3568

 

Uniprot Entry: Q01344

 

Construct Details: The recombinant human CD125/IL5RA ECD protein is expressed as a 326 amino acid protein consisting of Asp21 - Glu335 region of CD125 (UniProt accession #Q01344) and and a C-terminal His-tag. It contains 4 potential sites for N-linked glycosylation.

 

Source: Human cells stably expressing CD125 ECD and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

DLLPDEKISL LPPVNFTIKV TGLAQVLLQW KPNPDQEQRN VNLEYQVKIN APKEDDYETR 

        70         80         90        100        110        120 

ITESKCVTIL HKGFSASVRT ILQNDHSLLA SSWASAELHA PPGSPGTSIV NLTCTTNTTE 

       130        140        150        160        170        180 

DNYSRLRSYQ VSLHCTWLVG TDAPEDTQYF LYYRYGSWTE ECQEYSKDTL GRNIACWFPR 

       190        200        210        220        230        240 

TFILSKGRDW LAVLVNGSSK HSAIRPFDQL FALHAIDQIN PPLNVTAEIE GTRLSIQWEK 

       250        260        270        280        290        300 

PVSAFPIHCF DYEVKIHNTR NGYLQIEKLM TNAFISIIDD LSKYDVQVRA AVSSMCREAG 

       310        320 

LWSEWSQPIY VGNDESTGHH HHHHHH 

 

 

  

M.W.: Calculated molecular mass 37.2 kDa; estimated by SDS-PAGE under reducing condition: 1 major species of ~50-55 kDa with 2 additional minor species of ~70 and ~80 kDa, respectively

 

Calculated PI: 5.87

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  69245

  

Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Cell 66:1175-1184(1991)

2. J. Exp. Med. 175:341-351(1992)

3. Proc. Natl. Acad. Sci. 89:7041-7045(1992) 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.