NRP1 (neuropilin-1), one of the 2 members of the neuropilin family, is a single-pass type I transmembrane glycoprotein.  Both members of  neuropilins contain a large N-terminal extracellular region consisting of 2 complement-binding (CUB),  2 coagulation factor V/VIII (F5/8 type C), and 1 meprin (MAM) domains in addition to a transmembrane domain and a small cytoplasmic tail. These specific domains allow neuropilins to participate in several different types of signaling pathways that control cell survival, migration, and attraction. Neuropilins bind many ligands and various types of co-receptors including vascular endothelial growth factor (VEGF) and semaphoring (SEMA) family members.  The tandem CUB domains mediate the binding to SEMA, while the tandem F5/8 type C domains are responsible for heparin and VEGF binding. NRP1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. NRP1 mediates the chemorepulsant activity of semaphorins. NRP1 binds to SEMA3A, the PLGF-2 isoform of PGF, the VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. NRP1 may regulate VEGF-induced angiogenesis.  The soluble isoform of NRP1 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165 and bind various members of the SEMA family. Its expression has an adverse effect on blood vessel number and integrity.

NRP1 (neuropilin-1), one of the 2 members of the neuropilin family, is a single-pass type I transmembrane glycoprotein.  Both members of  neuropilins contain a large N-terminal extracellular region consisting of 2 complement-binding (CUB),  2 coagulation factor V/VIII (F5/8 type C), and 1 meprin (MAM) domains in addition to a transmembrane domain and a small cytoplasmic tail. These specific domains allow neuropilins to participate in several different types of signaling pathways that control cell survival, migration, and attraction. Neuropilins bind many ligands and various types of co-receptors including vascular endothelial growth factor (VEGF) and semaphoring (SEMA) family members.  The tandem CUB domains mediate the binding to SEMA, while the tandem F5/8 type C domains are responsible for heparin and VEGF binding. NRP1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. NRP1 mediates the chemorepulsant activity of semaphorins. NRP1 binds to SEMA3A, the PLGF-2 isoform of PGF, the VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. NRP1 may regulate VEGF-induced angiogenesis.  The soluble isoform of NRP1 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165 and bind various members of the SEMA family. Its expression has an adverse effect on blood vessel number and integrity.

 

Product Details

 

Gene Symbol: NRP1; NP1; NRP; BDCA4; CD304; VEGF165R; neuropilin-1; neuropilin1

 

NCBI Gene ID: 8829

 

Uniprot Entry: O14786

 

Construct Details: The recombinant human NRP1 ECD protein is expressed as an 842-amino acid protein consisting of Phe22 -  Lys852 region of NRP1 and a C-terminal His-tag. NRP1 ECD contains 5 potential N-linked glycosylation sites.

 

Source: Human cells stably expressing NRP1 ECD and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

FRNDKCGDTI KIESPGYLTS PGYPHSYHPS EKCEWLIQAP DPYQRIMINF NPHFDLEDRD 

        70         80         90        100        110        120 

CKYDYVEVFD GENENGHFRG KFCGKIAPPP VVSSGPFLFI KFVSDYETHG AGFSIRYEIF 

       130        140        150        160        170        180 

KRGPECSQNY TTPSGVIKSP GFPEKYPNSL ECTYIVFVPK MSEIILEFES FDLEPDSNPP 

       190        200        210        220        230        240 

GGMFCRYDRL EIWDGFPDVG PHIGRYCGQK TPGRIRSSSG ILSMVFYTDS AIAKEGFSAN 

       250        260        270        280        290        300 

YSVLQSSVSE DFKCMEALGM ESGEIHSDQI TASSQYSTNW SAERSRLNYP ENGWTPGEDS 

       310        320        330        340        350        360 

YREWIQVDLG LLRFVTAVGT QGAISKETKK KYYVKTYKID VSSNGEDWIT IKEGNKPVLF 

       370        380        390        400        410        420 

QGNTNPTDVV VAVFPKPLIT RFVRIKPATW ETGISMRFEV YGCKITDYPC SGMLGMVSGL 

       430        440        450        460        470        480 

ISDSQITSSN QGDRNWMPEN IRLVTSRSGW ALPPAPHSYI NEWLQIDLGE EKIVRGIIIQ 

       490        500        510        520        530        540 

GGKHRENKVF MRKFKIGYSN NGSDWKMIMD DSKRKAKSFE GNNNYDTPEL RTFPALSTRF 

       550        560        570        580        590        600 

IRIYPERATH GGLGLRMELL GCEVEAPTAG PTTPNGNLVD ECDDDQANCH SGTGDDFQLT 

       610        620        630        640        650        660 

GGTTVLATEK PTVIDSTIQS EFPTYGFNCE FGWGSHKTFC HWEHDNHVQL KWSVLTSKTG 

       670        680        690        700        710        720 

PIQDHTGDGN FIYSQADENQ KGKVARLVSP VVYSQNSAHC MTFWYHMSGS HVGTLRVKLR 

       730        740        750        760        770        780 

YQKPEEYDQL VWMAIGHQGD HWKEGRVLLH KSLKLYQVIF EGEIGKGNLG GIAVDDISIN 

       790        800        810        820        830        840 

NHISQEDCAK PADLDKKNPE IKIDETGSTP GYEGEGEGDK NISRKPGNVL KSTGHHHHHH 

 

HH 

 

  

M.W.: Calculated molecular mass (kDa): 94.7; Estimated by SDS-PAGE under reducing condition (kDa): 100-110

 

Calculated PI: 5.84

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  146775

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).  The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Not available

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.