Human B7-H2 Full-length Gene in Lentivector, Endotoxin-free DNA

Product ID: pLTC-B7-H2 (SKU#: LTP0797)

Price:

$796.00

Description

B7H2 (B7 homolog 2), also known as CD275, B7RP1 (B7related protein) and ICOSL (ICOS Ligand), is a single-pass type I transmembrane glycoprotein that belongs to the B7 family of the immunoglobulin (Ig) superfamily. Like other B7 family members, B7-H25 contains 1 Ig-like C2-type domain and 1 Ig-like V-type domain in the extracellular region. Among the family members, they share about 20-25% amino acid identity. B7-H2 is expressed on antigen presenting cells such as B cells, macrophages, monocytes, and dendritic cells. B7-H2 has been identified as the ligand for ICOS (also known as CD278), a member of the CD28/CTLA4 family of co-stimulatory receptors. The receptor binding of B7-H2 is mediated by the IgV domain and requires the IgC domain for maintaining the structural integrity of the protein. The B7-H2/ICOS interaction plays roles in T cell dependent B cell activation and Th (T helper) cell differentiation, leading to both positive and negative effects on immune responses. In human, B7-H2 also binds to CD28 and CTLA4, and its interaction with CD28 can co-stimulate T cells. B7-H2 contributes to the development of allergic asthma and other autoimmune conditions. A soluble form of human B7H2 is elevated in the circulation of patients with systemic lupus erythematosus. In the thyroid, B7H2 is upregulated on thyrocytes during inflammation and promotes the production of thryoid hormones. It is reported that mouse and human B7-H2 show crossspecies binding to ICOS.

Product Details

Gene Symbol: B7-H2; CD275; ICOSL; B7H2; GL50; ICOSLG; B7RP1; ICOS-L; LICOS; B7RP-1; KIAA0653

NCBI Gene ID: 23308

Uniprot Entry: O75144

Construct Details: Full length human B7-H2/CD275/ICOSL gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

Gene Insert Size: 909 (bp)

Gene Insert Sequence:

ATGCGGCTGGGCAGTCCTGGACTGCTCTTCCTGCTCTTCAGCAGCCTTCGAGCTGATACTCAGGAGAAGGAAGTCAGAGC

GATGGTAGGCAGCGACGTGGAGCTCAGCTGCGCTTGCCCTGAAGGAAGCCGTTTTGATTTAAATGATGTTTACGTATATT

GGCAAACCAGTGAGTCGAAAACCGTGGTGACCTACCACATCCCACAGAACAGCTCCTTGGAAAACGTGGACAGCCGCTAC

CGGAACCGAGCCCTGATGTCACCGGCCGGCATGCTGCGGGGCGACTTCTCCCTGCGCTTGTTCAACGTCACCCCCCAGGA

CGAGCAGAAGTTTCACTGCCTGGTGTTGAGCCAATCCCTGGGATTCCAGGAGGTTTTGAGCGTTGAGGTTACACTGCATG

TGGCAGCAAACTTCAGCGTGCCCGTCGTCAGCGCCCCCCACAGCCCCTCCCAGGATGAGCTCACCTTCACGTGTACATCC

ATAAACGGCTACCCCAGGCCCAACGTGTACTGGATCAATAAGACGGACAACAGCCTGCTGGACCAGGCTCTGCAGAATGA

CACCGTCTTCTTGAACATGCGGGGCTTGTATGACGTGGTCAGCGTGCTGAGGATCGCACGGACCCCCAGCGTGAACATTG

GCTGCTGCATAGAGAACGTGCTTCTGCAGCAGAACCTGACTGTCGGCAGCCAGACAGGAAATGACATCGGAGAGAGAGAC

AAGATCACAGAGAATCCAGTCAGTACCGGCGAGAAAAACGCGGCCACGTGGAGCATCCTGGCTGTCCTGTGCCTGCTTGT

GGTCGTGGCGGTGGCCATAGGCTGGGTGTGCAGGGACCGATGCCTCCAACACAGCTATGCAGGTGCCTGGGCTGTGAGTC

CGGAGACAGAGCTCACTGGCCACGTTTGA

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

Storage

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

References