CD160, also known as Natural killer cell receptor BY55, is a GPI-anchored cell surface protein. As a member of the Ig (immunoglobulin) superfamily, CD160 contains 1 Ig-like V-type domain in its extracellular region. Its expression is restricted to functional NK and T cytotoxic lymphocytes. CD160 is expressed as a disulfide-linked multimer on the cell surface. CD160 is a receptor showing broad specificity for both classical and non-classical MHC class I molecules. CD160 is known to bind HLA-G1, HLA-C, and HVEM. CD160 binding to HLA-G1 initiates apoptosis. CD160 signaling in response to HLA-C binding promotes cytokine secretion. CD160 is up regulated on CD4+ T cells following activation. CD160 may function as a co-activator receptor for CD3-induced proliferation of T cells and CD4+ CD160+ lymphocyte subset may play a role in the pathogenesis of skin inflammation. CD160 engagement by HVEM (expressed by APC) serves to block a simultaneous LIGHT stimulation of HVEM that promotes receptor expression and cytokine release. Activated NK lymphocytes may release a soluble form of CD160 that impairs the MHC-I-specific cytotoxic T lymphocyte responsiveness.

 

CD160, also known as Natural killer cell receptor BY55, is a GPI-anchored cell surface protein. As a member of the Ig (immunoglobulin) superfamily, CD160 contains 1 Ig-like V-type domain in its extracellular region. Its expression is restricted to functional NK and T cytotoxic lymphocytes. CD160 is expressed as a disulfide-linked multimer on the cell surface. CD160 is a receptor showing broad specificity for both classical and non-classical MHC class I molecules. CD160 is known to bind HLA-G1, HLA-C, and HVEM. CD160 binding to HLA-G1 initiates apoptosis. CD160 signaling in response to HLA-C binding promotes cytokine secretion. CD160 is up regulated on CD4+ T cells following activation. CD160 may function as a co-activator receptor for CD3-induced proliferation of T cells and CD4+ CD160+ lymphocyte subset may play a role in the pathogenesis of skin inflammation. CD160 engagement by HVEM (expressed by APC) serves to block a simultaneous LIGHT stimulation of HVEM that promotes receptor expression and cytokine release. Activated NK lymphocytes may release a soluble form of CD160 that impairs the MHC-I-specific cytotoxic T lymphocyte responsiveness.

  

Product Details

 

Gene Symbol: CD160; NK1; BY55; NK28

  

NCBI Gene ID: 11126

 

Uniprot Entry: O95971

 

Construct Details: The recombinant human CD160-Fc fusion is expressed as a 359 amino acid protein consisting of Ile27 - Thr157 region of CD160 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions

 

Source: Human cells stably expressing human CD160-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

 

        10         20         30         40         50         60 

INITSSASQE GTRLNLICTV WHKKEEAEGF VVFLCKDRSG DCSPETSLKQ LRLKRDPGID 

        70         80         90        100        110        120 

GVGEISSQLM FTISQVTPLH SGTYQCCARS QKSGIRLQGH FFSILFTETG NYTVTGLKQR 

       130        140        150        160        170        180 

QHLEFSHNEG TSTGTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH  

       190        200        210        220        230        240 

EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL 

       250        260        270        280        290        300 

PAPIEKTISK AKGQPREPQV YTLPPSREEM TKNQVSLTCL VKGFYPSDIA VEWESNGQPE 

       310        320        330        340        350 

NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK 

 

  

M.W.: Calculated molecular mass (kDa): 40.2 Estimated by SDS-PAGE under reducing condition (kDa): 45-50

 

Calculated PI: 7.67

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 44515

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to human HVEM protein (SKU#FCL0096) in a functional ELISA.

 

 

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

1. J. Immunol. 161:2780 (1998)

2. J. Immunol. 162:1223 (1999)

3. J Immunol.173: 5349 (2004)

4. Immunol. Rev. 229:244 (2009)

 

 

  

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.