LFA3 (lymphocyte function-associated antigen 3), also known as Ag3 and CD58, is single-pass type I transmembrane or GPI-anchored glycoprotein belonging to the immunoglobulin superfamily (IgSF). LFA3 contains 1 Ig-like C2-type domain in its extracellular region. LFA3 is primarily expressed on Antigen Presenting Cells (APCs), particularly macrophages.  LFA3 is the ligand of the T-lymphocyte CD2 glycoprotein and functions in adhesion and activation of T lymphocytes. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and antigen-dependent interactions of T-lymphocytes with target cells. It is important in strengthening the adhesion between the T cells and Professional APCs. This adhesion occurs as part of the transitory initial encounters between T cells and APCs before T cell activation, when T cells are roaming the lymph nodes looking at the surface of APCs for peptide:MHC complexes that the T-cell receptors are reactive to. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA3+ cells and is important for the T-lymphocyte rosetting with erythrocytes. Polymorphisms in the LFA3 gene are associated with increased risk for multiple sclerosis.

LFA3 (lymphocyte function-associated antigen 3), also known as Ag3 and CD58, is single-pass type I transmembrane or GPI-anchored glycoprotein belonging to the immunoglobulin superfamily (IgSF). LFA3 contains 1 Ig-like C2-type domain in its extracellular region. LFA3 is primarily expressed on Antigen Presenting Cells (APCs), particularly macrophages.  LFA3 is the ligand of the T-lymphocyte CD2 glycoprotein and functions in adhesion and activation of T lymphocytes. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and antigen-dependent interactions of T-lymphocytes with target cells. It is important in strengthening the adhesion between the T cells and Professional APCs. This adhesion occurs as part of the transitory initial encounters between T cells and APCs before T cell activation, when T cells are roaming the lymph nodes looking at the surface of APCs for peptide:MHC complexes that the T-cell receptors are reactive to. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA3+ cells and is important for the T-lymphocyte rosetting with erythrocytes. Polymorphisms in the LFA3 gene are associated with increased risk for multiple sclerosis.

 

Product Details

 

Gene Symbol: CD58; ag3; LFA3; LFA-3

 

NCBI Gene ID: 965

 

Uniprot Entry: P19256

 

Construct Details: The recombinant human LFA3-Fc is expressed as a 319-amino acid protein consisting of Phe29 - Val120 region of LFA3 with a Fc-fusion from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image above).   

 

Source: Human cells stably expressing human LFA3-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

FSQQIYGVVY GNVTFHVPSN VPLKEVLWKK QKDKVAELEN SEFRAFSSFK NRVYLDTVSG 

        70         80         90        100        110        120 

SLTIYNLTSS DEDEYEMESP NITDTMKFFL YVDKTHTCPP CPAPELLGGP SVFLFPPKPK 

       130        140        150        160        170        180 

DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV 

       190        200        210        220        230        240 

LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL 

       250        260        270        280        290        300 

VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM 

       310 

HEALHNHYTQ KSLSLSPGK 

 

  

M.W.: Calculated molecular mass (kDa): 36.2 Estimated by SDS-PAGE under reducing condition (kDa): 50-55.

 

Calculated PI: 6.11

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 50225

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to its receptor human CD2 (SKU#FCL2552) and increases T cell proliferation and cytokine secretion in the presence of anti-CD3 antibody.

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. J. Exp. Med. 166:923 (1987)

2. Nature 329:840 (1987)

3. J. Immunol. 160:2862 (1998)

4. Cell 97:791 (1999)

5. Proc. Natl. Acad. Sci. 96:4289 (1999) 

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2774B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.