CD2, also known as T-cell surface antigen T11/Leu-5 and SRBC (sheep red blood cell receptor), is a single-pass type I transmembrane protein of the immunoglobulin superfamily (IgSF). CD2 contains one Ig-like C2-type motif and one Ig-like V-type motifs in the extracellular domain (ECD). CD2 is a surface antigen of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells.  CD2 is one of the earliest T-cell markers present on more than 95% of thymocytes. CD2 is also found on some natural killer (NK) cells but not on B lymphocytes. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or closely associated with it.  CD2 interacts with lymphocyte function-associated antigen (CD58/LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells and its cytoplasmic domain is implicated in the signaling function by binding to intracellular proteins. CD2 may set quantitative thresholds in T cell activation both in vitro and in vivo. The complex network of CD2-CD58 signaling also plays an important role in enhancing the adhesion of T lymphocytes to target cells, and promoting hyperplasia and activation of T lymphocytes. 

CD2, also known as T-cell surface antigen T11/Leu-5 and SRBC (sheep red blood cell receptor), is a single-pass type I transmembrane protein of the immunoglobulin superfamily (IgSF). CD2 contains one Ig-like C2-type motif and one Ig-like V-type motifs in the extracellular domain (ECD). CD2 is a surface antigen of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells.  CD2 is one of the earliest T-cell markers present on more than 95% of thymocytes. CD2 is also found on some natural killer (NK) cells but not on B lymphocytes. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or closely associated with it.  CD2 interacts with lymphocyte function-associated antigen (CD58/LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells and its cytoplasmic domain is implicated in the signaling function by binding to intracellular proteins. CD2 may set quantitative thresholds in T cell activation both in vitro and in vivo. The complex network of CD2-CD58 signaling also plays an important role in enhancing the adhesion of T lymphocytes to target cells, and promoting hyperplasia and activation of T lymphocytes. 

 

Product Details

 

Gene Symbol: CD2; T11; SRBC; LFA-2; Leu-5

 

NCBI Gene ID: 914

 

Uniprot Entry: P06729

 

Construct Details: The recombinant human CD2-Fc is expressed as a 425-amino acid protein consisting of  Lys25 -  Asp209 region of CD2 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.   

 

Source:  Human cells stably expressing CD2-Fc growing in chemical-defined media with no animal components or antibiotics 

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

KEITNALETW GALGQDINLD IPSFQMSDDI DDIKWEKTSD KKKIAQFRKE KETFKEKDTY 

        70         80         90        100        110        120 

KLFKNGTLKI KHLKTDDQDI YKVSIYDTKG KNVLEKIFDL KIQERVSKPK ISWTCINTTL 

       130        140        150        160        170        180 

TCEVMNGTDP ELNLYQDGKH LKLSQRVITH KWTTSLSAKF KCTAGNKVSK ESSVEPVSCP 

       190        200        210        220        230        240 

EKGLDGSSTT ENLYFQGSTG THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV 

       250        260        270        280        290        300 

VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK  

       310        320        330        340        350        360 

VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSREEMTKNQ VSLTCLVKGF YPSDIAVEWE 

       370        380        390        400        410        420 

SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS 

  

LSPGK 

 

M.W.: Calculated molecular mass (kDa): 48.1; Estimated by SDS-PAGE under reducing condition (kDa): 60-65

 

Calculated PI: 8.18

 

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm): 65485

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to human CD58/LFA3 protein by ELISA and blocks CD58/LFA3’s binding to cell surface CD2 and mediated signaling activity.

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. J. Exp. Med. 166:923 (1987)

2. Nature 329:840 (1987) 

3. J. Immunol. 160:2862 (1998)

4. Cell 97:791 (1999) 

5. Proc. Natl. Acad. Sci. 96:4289 (1999)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2552B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.