LFA3 (lymphocyte function-associated antigen 3), also known as Ag3 and CD58, is single-pass type I transmembrane or GPI-anchored glycoprotein belonging to the immunoglobulin superfamily (IgSF). LFA3 contains 1 Ig-like C2-type domain in its extracellular region. LFA3 is primarily expressed on Antigen Presenting Cells (APCs), particularly macrophages.  LFA3 is the ligand of the T-lymphocyte CD2 glycoprotein and functions in adhesion and activation of T lymphocytes. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and antigen-dependent interactions of T-lymphocytes with target cells. It is important in strengthening the adhesion between the T cells and Professional APCs. This adhesion occurs as part of the transitory initial encounters between T cells and APCs before T cell activation, when T cells are roaming the lymph nodes looking at the surface of APCs for peptide:MHC complexes that the T-cell receptors are reactive to. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA3+ cells and is important for the T-lymphocyte rosetting with erythrocytes. Polymorphisms in the LFA3 gene are associated with increased risk for multiple sclerosis.

LFA3 (lymphocyte function-associated antigen 3), also known as Ag3 and CD58, is single-pass type I transmembrane or GPI-anchored glycoprotein belonging to the immunoglobulin superfamily (IgSF). LFA3 contains 1 Ig-like C2-type domain in its extracellular region. LFA3 is primarily expressed on Antigen Presenting Cells (APCs), particularly macrophages.  LFA3 is the ligand of the T-lymphocyte CD2 glycoprotein and functions in adhesion and activation of T lymphocytes. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and antigen-dependent interactions of T-lymphocytes with target cells. It is important in strengthening the adhesion between the T cells and Professional APCs. This adhesion occurs as part of the transitory initial encounters between T cells and APCs before T cell activation, when T cells are roaming the lymph nodes looking at the surface of APCs for peptide:MHC complexes that the T-cell receptors are reactive to. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA3+ cells and is important for the T-lymphocyte rosetting with erythrocytes. Polymorphisms in the LFA3 gene are associated with increased risk for multiple sclerosis.

 

Product Details

 

Gene Symbol: CD58; ag3; LFA3; LFA-3

 

NCBI Gene ID: 965

 

Uniprot Entry: P19256

 

Construct Details: Full length human LFA3 gene (encoding UniProt #P19256 isoform 1) is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter+/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 753 (bp)

 

Gene Insert Sequence:  

ATGGTTGCTGGGAGCGACGCGGGGCGGGCCCTGGGGGTCCTCAGCGTGGTCTGCCTGCTGCACTGCTTTG

GTTTCATCAGCTGTTTTTCCCAACAAATATATGGTGTTGTGTATGGGAATGTAACTTTCCATGTACCAAG

CAATGTGCCTTTAAAAGAGGTCCTATGGAAAAAACAAAAGGATAAAGTTGCAGAACTGGAAAATTCTGAA

TTCAGAGCTTTCTCATCTTTTAAAAATAGGGTTTATTTAGACACTGTGTCAGGTAGCCTCACTATCTACA

ACTTAACATCATCAGATGAAGATGAGTATGAAATGGAATCGCCAAATATTACTGATACCATGAAGTTCTT

TCTTTATGTGCTTGAGTCTCTTCCATCTCCCACACTAACTTGTGCATTGACTAATGGAAGCATTGAAGTC

CAATGCATGATACCAGAGCATTACAACAGCCATCGAGGACTTATAATGTACTCATGGGATTGTCCTATGG

AGCAATGTAAACGTAACTCAACCAGTATATATTTTAAGATGGAAAATGATCTTCCACAAAAAATACAGTG

TACTCTTAGCAATCCATTATTTAATACAACATCATCAATCATTTTGACAACCTGTATCCCAAGCAGCGGT

CATTCAAGACACAGATATGCACTTATACCCATACCATTAGCAGTAATTACAACATGTATTGTGCTGTATA

TGAATGGTATTCTGAAATGTGACAGAAAACCAGACAGAACCAACTCCAATTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. J. Exp. Med. 166:923 (1987)

2. Nature 329:840 (1987)

3. J. Immunol. 160:2862 (1998)

4. Cell 97:791 (1999)

5. Proc. Natl. Acad. Sci. 96:4289 (1999) 

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTP2774-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.