CD96, also known as Tactile (T cell-activated increased late expression), is a single pass type I transmembrane protein of the immunoglobulin superfamily (IgSF).  The extracellular domain (ECD) of CD96 contains 2 V-type and 1 C-type Ig-like domains, which are highly glycosylated.  An 80-kDa soluble form of CD96 is elevated in human serum during chronic hepatitis B. CD96 is expressed on activated T, NK and a subpopulation of B cells as well as on acute myeloid leukemia and  myelodysplastic stem cells. CD96 is allocated to the repertoire of human NK receptors. NK cells recognize poliovirus receptor (PVR) or CD155, a nectins and nectin-like protein family member serve to mediate cell-cell adhesion and cell migration. CD96 may be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response.  CD96 promotes NK cell adhesion to target cells expressing PVR, stimulates cytotoxicity of activated NK cells. The most N-terminal Ig-like domain of CD96 binds to PVR.  On NK cells, co-stimulatory molecules CD96 and DNAM-1 (CD226) are thought to have paired activity with inhibitory TIGIT, all of which bind CD155 and various members of nectin family.   CD96 may function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation.  CD96 mutations may cause a form of the C syndrome, a set of developmental anomalies in cranial bone, skin and viscera.  

CD96, also known as Tactile (T cell-activated increased late expression), is a single pass type I transmembrane protein of the immunoglobulin superfamily (IgSF).  The extracellular domain (ECD) of CD96 contains 2 V-type and 1 C-type Ig-like domains, which are highly glycosylated.  An 80-kDa soluble form of CD96 is elevated in human serum during chronic hepatitis B. CD96 is expressed on activated T, NK and a subpopulation of B cells as well as on acute myeloid leukemia and  myelodysplastic stem cells. CD96 is allocated to the repertoire of human NK receptors. NK cells recognize poliovirus receptor (PVR) or CD155, a nectins and nectin-like protein family member serve to mediate cell-cell adhesion and cell migration. CD96 may be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response.  CD96 promotes NK cell adhesion to target cells expressing PVR, stimulates cytotoxicity of activated NK cells. The most N-terminal Ig-like domain of CD96 binds to PVR.  On NK cells, co-stimulatory molecules CD96 and DNAM-1 (CD226) are thought to have paired activity with inhibitory TIGIT, all of which bind CD155 and various members of nectin family.   CD96 may function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation.  CD96 mutations may cause a form of the C syndrome, a set of developmental anomalies in cranial bone, skin and viscera.  

 

Product Details

 

Gene Symbol: CD96; TACTILE

 

NCBI Gene ID: 10225

 

Uniprot Entry: P40200

 

Construct Details: The recombinant human CD96-Fc fusion is expressed as a 719-amino acid protein consisting of  Val22 - Gly502 region of CD96 (Uniprot#P40200-2) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

  

Source: Human cells stably expressing human CD96-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

VWEKTVNTEE NVYATLGSDV NLTCQTQTVG FFVQMQWSKV TNKIDLIAVY HPQYGFYCAY 

        70         80         90        100        110        120 

GRPCESLVTF TETPENGSKW TLHLRNMSCS VSGRYECMLV LYPEGIQTKI YNLLIQTHVT 

       130        140        150        160        170        180 

ADEWNSNHTI EIEINQTLEI PCFQNSSSKI SSEFTYAWSV EDNGTQETLI SQNHLISNST 

       190        200        210        220        230        240 

LLKDRVKLGT DYRLHLSPVQ IFDDGRKFSC HIRVGPNKIL RSSTTVKVFA KPEIPVIVEN 

       250        260        270        280        290        300 

NSTDVLVERR FTCLLKNVFP KANITWFIDG SFLHDEKEGI YITNEERKGK DGFLELKSVL  

       310        320        330        340        350        360 

TRVHSNKPAQ SDNLTIWCMA LSPVPGNKVW NISSEKITFL LGSEISSTDP PLSVTESTLD 

       370        380        390        400        410        420 

TQPSPASSVS PARYPATSSV TLVDVSALRP NTTPQPSNSS MTTRGFNYPW TSSGTDTKKS 

       430        440        450        460        470        480 

VSRIPSETYS SSPSGAGSTL HDNVFTSTAR AFSEVPTTAN GSTKTNHVHI TGIVVNKPKD 

       490        500        510        520        530        540 

GSTTENLYFQ GSTGTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH 

       550        560        570        580        590        600 

EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL 

       610        620        630        640        650        660 

PAPIEKTISK AKGQPREPQV YTLPPSREEM TKNQVSLTCL VKGFYPSDIA VEWESNGQPE 

       670        680        690        700        710 

NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK 

 

  

M.W.: Calculated molecular mass (kDa): 80.1 Estimated by SDS-PAGE under reducing condition (kDa): 100-110.

 

Calculated PI: 6.47

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 108135

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds PVR/CD155/NECL5 (SKU#FCL1077) and neutralizes its mediated signaling activity.

 

 

 

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. J. Immunol. 148:2600 (1992)

2. J. Immunol. 172:3994 (2004)

3. Proc. Natl. Acad. Sci. 104:11008 (2007)

4. Am. J. Hum. Genet. 81:835 (2007)

5. J. Biol. Chem. 284:2235 (2009)

 

  

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2473B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.