NECL3 (Nectin-like protein 3), also known as IgSF4D and CADM2 (cell adhesion molecule 2), is a single pass type I transmembrane glycoprotein that belongs to the Nectin family of the Ig (immunoglobulin) superfamily.  Most Nectin family members function as cell adhesion molecules (CAMs) located on the cell surface and involved with the binding with other cells or with the extracellular matrix (ECM). Like other Nectin members, NECL3 contains 1 Ig-like V-type domain and 2 Ig-like C2-type domains in the extracellular region that interacts either with other CAMs of the same kind (homophilic binding) or with other CAMs or the extracellular matrix (heterophilic binding) in a Ca2+-independent manner. The expression and function of NECL3 has not been well characterized.  It is found in the axoplasm of myelinated axons.  It may play an mportant role in synapse organization, providing regulated trans-synaptic adhesion. NECL3 contains a cytoplasmic domain with protein 4.1 and PDZ domain binding sites, allowing connections to adaptor molecules that are critical for synaptogenesis.

NECL3 (Nectin-like protein 3), also known as IgSF4D and CADM2 (cell adhesion molecule 2), is a single pass type I transmembrane glycoprotein that belongs to the Nectin family of the Ig (immunoglobulin) superfamily.  Most Nectin family members function as cell adhesion molecules (CAMs) located on the cell surface and involved with the binding with other cells or with the extracellular matrix (ECM). Like other Nectin members, NECL3 contains 1 Ig-like V-type domain and 2 Ig-like C2-type domains in the extracellular region that interacts either with other CAMs of the same kind (homophilic binding) or with other CAMs or the extracellular matrix (heterophilic binding) in a Ca2+-independent manner. The expression and function of NECL3 has not been well characterized.  It is found in the axoplasm of myelinated axons.  It may play an mportant role in synapse organization, providing regulated trans-synaptic adhesion. NECL3 contains a cytoplasmic domain with protein 4.1 and PDZ domain binding sites, allowing connections to adaptor molecules that are critical for synaptogenesis.

 

Product Details

 

Gene Symbol: NECL3; CADM2; IGSF4D; Necl-3; synCAM2; SynCAM-2

 

NCBI Gene ID: 253559

 

Uniprot Entry: Q8N3J6

 

Construct Details: Full length human NECL3 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. 

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1308 (bp)

 

Gene Insert Sequence:  

ATGATTTGGAAACGCAGCGCCGTTCTCCGCTTCTACAGTGTCTGCGGGCTCCTGCTACAAGGCAGCCAAGGGCAGTTTCC

ACTAACACAGAATGTAACCGTTGTTGAAGGTGGAACTGCAATTTTGACCTGCAGGGTTGATCAAAATGATAACACCTCCC

TCCAGTGGTCAAATCCAGCTCAACAGACTCTGTACTTTGACGACAAGAAAGCTTTAAGGGACAATAGGATCGAGCTGGTT

CGCGCTTCCTGGCATGAATTGAGTATTAGTGTCAGTGATGTGTCTCTCTCTGATGAAGGACAGTACACCTGTTCTTTATT

TACAATGCCTGTCAAAACTTCCAAGGCATATCTCACCGTTCTGGGTGTTCCTGAAAAGCCTCAGATTAGTGGATTCTCAT

CACCAGTTATGGAGGGTGACTTGATGCAGCTGACTTGCAAAACATCTGGTAGTAAACCTGCAGCTGATATAAGATGGTTC

AAAAATGACAAAGAGATTAAAGATGTAAAATATTTAAAAGAAGAGGATGCAAATCGCAAGACATTCACTGTCAGCAGCAC

ACTGGACTTCCGAGTGGACCGGAGTGATGATGGAGTGGCGGTCATCTGCAGAGTAGATCACGAATCCCTCAATGCCACCC

CTCAGGTAGCCATGCAGGTGCTAGAAATACACTATACACCATCAGTTAAGATTATACCATCGACTCCTTTTCCACAAGAA

GGACAGCCTTTAATTTTGACTTGTGAATCCAAAGGAAAACCACTGCCAGAACCTGTTTTGTGGACAAAGGATGGCGGAGA

ATTACCAGATCCTGACCGAATGGTTGTGAGTGGTAGGGAGCTAAACATTCTTTTCCTGAACAAAACGGATAATGGTACAT

ATCGATGTGAAGCCACAAACACCATTGGCCAAAGCAGTGCGGAATATGTTCTCATTGTGCATGATGTTCCCAACACTTTG

CTTCCCACTACTATCATCCCCTCCCTTACCACTGCAACAGTCACAACCACTGTAGCCATAACAACCAGCCCAACCACATC

TGCAACAACCAGCAGCATCAGAGATCCTAATGCTTTGGCTGGCCAGAATGGCCCTGACCATGCTCTCATAGGAGGAATAG

TGGCTGTAGTTGTATTTGTCACGCTGTGTTCTATCTTTCTGCTTGGTCGATATCTGGCAAGGCATAAAGGAACGTATTTA

ACAAATGAAGCTAAAGGAGCTGAAGATGCACCAGATGCTGATACAGCCATTATCAATGCTGAAGGCAGCCAAGTCAATGC

TGAAGAGAAAAAAGAGTATTTCATTTAA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Curr. Opin. Cell Biol. 16:513 (2004).  

2. J. Cell Sci. 118:1267 (2004).  

3. Genomics 87:139 (2006).  

4. BMC Neurosci. 8:90-90(2007).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTP1145-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.