IL13RA2 (Interleukin-13 receptor subunit alpha-2), also known as CD213a2 and IL13BP (IL13-binding protein), is a single-pass type I transmembrane protein belonging to the type I cytokine receptor family. Like IL13RA1, the extracellular domain (ECD) of IL13RA1 contains 3 fibronectin type III domains, D1, D2, and D3, of which the last two comprise the cytokine receptor homology modules (CRHs).  In contrast to IL13RA1, IL13RA2 binds IL-13 with a high affinity but lacks cytoplasmic domain, and does not appear to function as a signal mediator. IL-13RA2 is widely expressed and detected on many types of cancer cells including glioblastoma, AIDS Kaposi's sarcoma, and head & neck cancer. IL-13 binds two types of receptor: (1) a heterodimer of the IL13 RA1 and the IL4RA, transducing the IL13-dependent proliferative signal; and (2) the IL13RA2, acting as a nonsignaling "decoy" receptor by binding and internalizing IL13. IL13RA2 can also block IL4-induced responses by inhibiting IL4 bound IL13RA1/IL4RA receptor complexes even though it does not bind IL 4 directly. Aside from its decoy function, IL13-IL13RA2 binding directly promotes the development of tissue fibrosis. IL13RA2 may also play an important role in the Th2 polarized immune responses characteristic of a variety of pathologies, including parasitic infections and allergic asthma. IL-13Rα2 exists both on the cell membrane and in soluble form which are generated from distinct transcripts.

IL13RA2 (Interleukin-13 receptor subunit alpha-2), also known as CD213a2 and IL13BP (IL13-binding protein), is a single-pass type I transmembrane protein belonging to the type I cytokine receptor family. Like IL13RA1, the extracellular domain (ECD) of IL13RA1 contains 3 fibronectin type III domains, D1, D2, and D3, of which the last two comprise the cytokine receptor homology modules (CRHs).  In contrast to IL13RA1, IL13RA2 binds IL-13 with a high affinity but lacks cytoplasmic domain, and does not appear to function as a signal mediator. IL-13RA2 is widely expressed and detected on many types of cancer cells including glioblastoma, AIDS Kaposi's sarcoma, and head & neck cancer. IL-13 binds two types of receptor: (1) a heterodimer of the IL13 RA1 and the IL4RA, transducing the IL13-dependent proliferative signal; and (2) the IL13RA2, acting as a nonsignaling "decoy" receptor by binding and internalizing IL13. IL13RA2 can also block IL4-induced responses by inhibiting IL4 bound IL13RA1/IL4RA receptor complexes even though it does not bind IL 4 directly. Aside from its decoy function, IL13-IL13RA2 binding directly promotes the development of tissue fibrosis. IL13RA2 may also play an important role in the Th2 polarized immune responses characteristic of a variety of pathologies, including parasitic infections and allergic asthma. IL-13Rα2 exists both on the cell membrane and in soluble form which are generated from distinct transcripts.

 

Product Details

 

Gene Symbol: IL13RA2; CD213a2; IL-13RA2; IL13BP; IL13R2 

 

NCBI Gene ID: 3598

 

Uniprot Entry: Q14627

 

Construct Details: The recombinant human IL13RA2-Fc fusion is expressed as a 552 amino acid protein consisting of Asp27 - Thr340 region of IL13RA2 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

   

Source: Human cells stably expressing human IL13RA2-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

DTEIKVNPPQ DFEIVDPGYL GYLYLQWQPP LSLDHFKECT VEYELKYRNI GSETWKTIIT 

        70         80         90        100        110        120 

KNLHYKDGFD LNKGIEAKIH TLLPWQCTNG SEVQSSWAET TYWISPQGIP ETKVQDMDCV 

       130        140        150        160        170        180 

YYNWQYLLCS WKPGIGVLLD TNYNLFYWYE GLDHALQCVD YIKADGQNIG CRFPYLEASD 

       190        200        210        220        230        240 

YKDFYICVNG SSENKPIRSS YFTFQLQNIV KPLPPVYLTF TRESSCEIKL KWSIPLGPIP 

       250        260        270        280        290        300 

ARCFDYEIEI REDDTTLVTA TVENETYTLK TTNETRQLCF VVRSKVNIYC SDDGIWSEWS 

       310        320        330        340        350        360 

DKQCWEGEDL SKKTSTTENL YFQGSTGTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR 

       370        380        390        400        410        420 

TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN 

       430        440        450        460        470        480 

GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR EEMTKNQVSL TCLVKGFYPS 

       490        500        510        520        530        540 

DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH 

       550 

YTQKSLSLSP GK

 

 

M.W.: Calculated molecular mass (kDa): 63.4; Estimated by SDS-PAGE under reducing condition (kDa): ~90

 

Calculated PI: 5.23

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 136805

 

Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to its human ligand IL13/IL4 and blocks IL13/IL4-induced signaling activity and proliferation of human erythroleukemic cells.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. J. Biol. Chem. 271:16921 (1996).

2. J. Immunol. 177:7905 (1998)

3. Annu. Rev. Immunol. 21:425 (2003).

4. Curr. Allergy Asthma Rep. 7:338 (2007).

5. J. Allergy Clin. Immunol. 122:625 (2008).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2454B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.