IL13RA1 (Interleukin-13 receptor subunit alpha-1), also known as CD213a1 and CT19 (cancer/testis antigen 19), is a subunit of the IL13 receptor complex. The human IL13RA1 is a single-pass type I transmembrane protein belonging to the type I cytokine receptor family. The extracellular domain (ECD) of IL13RA1 contains 3 fibronectin type III domains, D1, D2, and D3, of which the last two comprise the cytokine receptor homology modules (CRHs). IL13RA1 binds IL13 with a low affinity by itself and forms a functional receptor for IL13 together with IL4RA, a subunit shared by IL13 and IL4 receptors. The biological effects of IL13 and IL4 are closely related in part due to a shared receptor system.  IL-13 binds two types of receptor: (1) a heterodimer of the IL13 RA1 and the IL4RA, transducing the IL13-dependent proliferative signal; and (2) the IL13RA2, acting as a nonsignaling "decoy" receptor.  IL13RA1 also serves as an alternate accessory protein to the common cytokine receptor gamma chain for IL4 signaling, but cannot replace the function of IL2RG in enhancing IL2 binding activity. IL13RA1 has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4. 

IL13RA1 (Interleukin-13 receptor subunit alpha-1), also known as CD213a1 and CT19 (cancer/testis antigen 19), is a subunit of the IL13 receptor complex. The human IL13RA1 is a single-pass type I transmembrane protein belonging to the type I cytokine receptor family. The extracellular domain (ECD) of IL13RA1 contains 3 fibronectin type III domains, D1, D2, and D3, of which the last two comprise the cytokine receptor homology modules (CRHs). IL13RA1 binds IL13 with a low affinity by itself and forms a functional receptor for IL13 together with IL4RA, a subunit shared by IL13 and IL4 receptors. The biological effects of IL13 and IL4 are closely related in part due to a shared receptor system.  IL-13 binds two types of receptor: (1) a heterodimer of the IL13 RA1 and the IL4RA, transducing the IL13-dependent proliferative signal; and (2) the IL13RA2, acting as a nonsignaling "decoy" receptor.  IL13RA1 also serves as an alternate accessory protein to the common cytokine receptor gamma chain for IL4 signaling, but cannot replace the function of IL2RG in enhancing IL2 binding activity. IL13RA1 has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4. 

 

Product Details

 

Gene Symbol: IL13RA1; NR4; CT19; CD213A1; IL13R; IL13RA; IL-13Ra; IL13R1

 

NCBI Gene ID: 3597

 

Uniprot Entry: P78552

 

Construct Details: Full length human IL13RA1 gene (encoding UniProt #P78552) is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1284 (bp)

 

Gene Insert Sequence:  

ATGGAGTGGCCGGCGCGGCTCTGCGGGCTGTGGGCGCTGCTGCTCTGCGCCGGCGGCGGGGGCGGGGGCG

GGGGCGCCGCGCCTACGGAAACTCAGCCACCTGTGACAAATTTGAGTGTCTCTGTTGAAAACCTCTGCAC

AGTAATATGGACATGGAATCCACCCGAGGGAGCCAGCTCAAATTGTAGTCTATGGTATTTTAGTCATTTT

GGCGACAAACAAGATAAGAAAATAGCTCCGGAAACTCGTCGTTCAATAGAAGTACCCCTGAATGAGAGGA

TTTGTCTGCAAGTGGGGTCCCAGTGTAGCACCAATGAGAGTGAGAAGCCTAGCATTTTGGTTGAAAAATG

CATCTCACCCCCAGAAGGTGATCCTGAGTCTGCTGTGACTGAGCTTCAATGCATTTGGCACAACCTGAGC

TACATGAAGTGTTCTTGGCTCCCTGGAAGGAATACCAGTCCCGACACTAACTATACTCTCTACTATTGGC

ACAGAAGCCTGGAAAAAATTCATCAATGTGAAAACATCTTTAGAGAAGGCCAATACTTTGGTTGTTCCTT

TGATCTGACCAAAGTGAAGGATTCCAGTTTTGAACAACACAGTGTCCAAATAATGGTCAAGGATAATGCA

GGAAAAATTAAACCATCCTTCAATATAGTGCCTTTAACTTCCCGTGTGAAACCTGATCCTCCACATATTA

AAAACCTCTCCTTCCACAATGATGACCTATATGTGCAATGGGAGAATCCACAGAATTTTATTAGCAGATG

CCTATTTTATGAAGTAGAAGTCAATAACAGCCAAACTGAGACACATAATGTTTTCTACGTCCAAGAGGCT

AAATGTGAGAATCCAGAATTTGAGAGAAATGTGGAGAATACATCTTGTTTCATGGTCCCTGGTGTTCTTC

CTGATACTTTGAACACAGTCAGAATAAGAGTCAAAACAAATAAGTTATGCTATGAGGATGACAAACTCTG

GAGTAATTGGAGCCAAGAAATGAGTATAGGTAAGAAGCGCAATTCCACACTCTACATAACCATGTTACTC

ATTGTTCCAGTCATCGTCGCAGGTGCAATCATAGTACTCCTGCTTTACCTAAAAAGGCTCAAGATTATTA

TATTCCCTCCAATTCCTGATCCTGGCAAGATTTTTAAAGAAATGTTTGGAGACCAGAATGATGATACTCT

GCACTGGAAGAAGTACGACATCTATGAGAAGCAAACCAAGGAGGAAACCGACTCTGTAGTGCTGATAGAA

AACCTGAAGAAAGCCTCTCAGTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. J. Biol. Chem. 271:29265 (1996)

2. FEBS Lett. 401:163 (1997) 

3. J. Immunol. 161:2317 (1998)

4. Annu. Rev. Immunol. 21:425 (2003).

5. Cell 132:259 (2008)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTP2453-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.