IL13RA1 (Interleukin-13 receptor subunit alpha-1), also known as CD213a1 and CT19 (cancer/testis antigen 19), is a subunit of the IL13 receptor complex. The human IL13RA1 is a single-pass type I transmembrane protein belonging to the type I cytokine receptor family. The extracellular domain (ECD) of IL13RA1 contains 3 fibronectin type III domains, D1, D2, and D3, of which the last two comprise the cytokine receptor homology modules (CRHs). IL13RA1 binds IL13 with a low affinity by itself and forms a functional receptor for IL13 together with IL4RA, a subunit shared by IL13 and IL4 receptors. The biological effects of IL13 and IL4 are closely related in part due to a shared receptor system.  IL-13 binds two types of receptor: (1) a heterodimer of the IL13 RA1 and the IL4RA, transducing the IL13-dependent proliferative signal; and (2) the IL13RA2, acting as a nonsignaling "decoy" receptor.  IL13RA1 also serves as an alternate accessory protein to the common cytokine receptor gamma chain for IL4 signaling, but cannot replace the function of IL2RG in enhancing IL2 binding activity. IL13RA1 has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4. 

IL13RA1 (Interleukin-13 receptor subunit alpha-1), also known as CD213a1 and CT19 (cancer/testis antigen 19), is a subunit of the IL13 receptor complex. The human IL13RA1 is a single-pass type I transmembrane protein belonging to the type I cytokine receptor family. The extracellular domain (ECD) of IL13RA1 contains 3 fibronectin type III domains, D1, D2, and D3, of which the last two comprise the cytokine receptor homology modules (CRHs). IL13RA1 binds IL13 with a low affinity by itself and forms a functional receptor for IL13 together with IL4RA, a subunit shared by IL13 and IL4 receptors. The biological effects of IL13 and IL4 are closely related in part due to a shared receptor system.  IL-13 binds two types of receptor: (1) a heterodimer of the IL13 RA1 and the IL4RA, transducing the IL13-dependent proliferative signal; and (2) the IL13RA2, acting as a nonsignaling "decoy" receptor.  IL13RA1 also serves as an alternate accessory protein to the common cytokine receptor gamma chain for IL4 signaling, but cannot replace the function of IL2RG in enhancing IL2 binding activity. IL13RA1 has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4. 

 

Product Details

 

Gene Symbol: IL13RA1; NR4; CT19; CD213A1; IL13R; IL13RA; IL-13Ra; IL13R1

 

NCBI Gene ID: 3597

 

Uniprot Entry: P78552

 

Construct Details: The recombinant human IL13RA1-Fc fusion is expressed as a 560 amino acid protein consisting of Gly22 - Thr343 region of IL13RA1 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

   

Source: Human cells stably expressing human IL13RA1-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

GGGGAAPTET QPPVTNLSVS VENLCTVIWT WNPPEGASSN CSLWYFSHFG DKQDKKIAPE 

        70         80         90        100        110        120 

TRRSIEVPLN ERICLQVGSQ CSTNESEKPS ILVEKCISPP EGDPESAVTE LQCIWHNLSY 

       130        140        150        160        170        180 

MKCSWLPGRN TSPDTNYTLY YWHRSLEKIH QCENIFREGQ YFGCSFDLTK VKDSSFEQHS  

       190        200        210        220        230        240 

VQIMVKDNAG KIKPSFNIVP LTSRVKPDPP HIKNLSFHND DLYVQWENPQ NFISRCLFYE 

       250        260        270        280        290        300 

VEVNNSQTET HNVFYVQEAK CENPEFERNV ENTSCFMVPG VLPDTLNTVR IRVKTNKLCY 

       310        320        330        340        350        360 

EDDKLWSNWS QEMSIGKKRN STSTTENLYF QGSTGTHTCP PCPAPELLGG PSVFLFPPKP 

       370        380        390        400        410        420 

KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT 

       430        440        450        460        470        480 

VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSREE MTKNQVSLTC 

       490        500        510        520        530        540 

LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV 

       550        560 

MHEALHNHYT QKSLSLSPGK 

  

M.W.: Calculated molecular mass (kDa): 63.6; Estimated by SDS-PAGE under reducing condition (kDa): 90-100

 

Calculated PI: 5.98

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 102425

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to its human ligand IL13/IL4 and blocks IL13/IL4-induced signaling activity and proliferation of human erythroleukemic cells.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. J. Biol. Chem. 271:29265 (1996)

2. FEBS Lett. 401:163 (1997) 

3. J. Immunol. 161:2317 (1998)

4. Annu. Rev. Immunol. 21:425 (2003).

5. Cell 132:259 (2008)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2453-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.