There are 3 classes of receptors for the Fc region of IgG (FcγRs): FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16), each with multiple isoforms.  All these receptors are members of the Ig superfamily (IgSF) and they play important roles in the activation or inhibition of immune responses. FcγRI (CD64) is a high-affinity receptor for IgG while both FcγRII and FcγRIII are low-affinity receptors that bind IgG in the form of immune complexes. CD64 is single pass transmembrane protein with 3 extracellular Ig-like C2 type motifs. CD64 is expressed constitutively on monocytes and macrophages and can be induced on neutrophils and eosinophils.  Its expression is up-regulated during bacterial infections and sepsis. CD64 functions in both innate and adaptive immune responses.  The high affinity recognition of IgG by Fc gamma  RI permits the triggering of effector responses at low IgG concentrations typical of early immune responses. Three highly homologous genes (A, B, and C) with 98% DNA sequence identity have been identified for the human CD64 group. The genes for isoform B and C contain stop codons within their membrane proximal Ig-like motifs generating possible secreted receptors.  A splice variant of CD64 has a deletion of the membrane-proximal Ig-like domain resulting in a putative transmembrane receptor.

There are 3 classes of receptors for the Fc region of IgG (FcγRs): FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16), each with multiple isoforms.  All these receptors are members of the Ig superfamily (IgSF) and they play important roles in the activation or inhibition of immune responses. FcγRI (CD64) is a high-affinity receptor for IgG while both FcγRII and FcγRIII are low-affinity receptors that bind IgG in the form of immune complexes. CD64 is single pass transmembrane protein with 3 extracellular Ig-like C2 type motifs. CD64 is expressed constitutively on monocytes and macrophages and can be induced on neutrophils and eosinophils.  Its expression is up-regulated during bacterial infections and sepsis. CD64 functions in both innate and adaptive immune responses.  The high affinity recognition of IgG by Fc gamma  RI permits the triggering of effector responses at low IgG concentrations typical of early immune responses. Three highly homologous genes (A, B, and C) with 98% DNA sequence identity have been identified for the human CD64 group. The genes for isoform B and C contain stop codons within their membrane proximal Ig-like motifs generating possible secreted receptors.  A splice variant of CD64 has a deletion of the membrane-proximal Ig-like domain resulting in a putative transmembrane receptor.

 

Product Details

 

Gene Symbol: CD64; FCGR1A; FCRI; CD64A; FCG1; FCGR1; IGFR1; FCGRI

 

NCBI Gene ID: 2209

 

Uniprot Entry: P12314

 

Construct Details: The recombinant human CD64 ECD is expressed as a 285 amino acid protein consisting of Gln16 - Val289 region of CD64 (UniProt accession #P12314) and a C-terminal His-tag. It contains 7 potential sites for N-linked glycosylation.

 

Source:  Human cells stably expressing CD64 ECD growing in chemical-defined media with no animal components or antibiotics 

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

QVDTTKAVIT LQPPWVSVFQ EETVTLHCEV LHLPGSSSTQ WFLNGTATQT STPSYRITSA 

        70         80         90        100        110        120 

SVNDSGEYRC QRGLSGRSDP IQLEIHRGWL LLQVSSRVFT EGEPLALRCH AWKDKLVYNV 

       130        140        150        160        170        180 

LYYRNGKAFK FFHWNSNLTI LKTNISHNGT YHCSGMGKHR YTSAGISVTV KELFPAPVLN 

       190        200        210        220        230        240 

ASVTSPLLEG NLVTLSCETK LLLQRPGLQL YFSFYMGSKT LRGRNTSSEY QILTARREDS  

       250        260        270        280 

GLYWCEAATE DGNVLKRSPE LELQVLGLQL PTPVSTGHHH HHHHH 

 

M.W.: Calculated molecular mass (kDa): 32.1; Estimated by SDS-PAGE under reducing condition (kDa): 50-55

 

Calculated PI: 8.71

 

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm): 49765

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds human IgG or Fc-fusion proteins with high affinity as measured by a functional ELISA (estimated Kd of 0.1-0.2 nM) 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Immunol. Today 14:215 (1993).

2. J Biol Chem. 270 (43): 25762 (1995)

3. Science 290:84 (2000)

4. Annu. Rev. Immunol.19:275 (2001).

5. Nature Rev. Immunol. 2:580 (2002)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2491-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.