PSCA (prostate stem cell antigen) is a glycosylphosphatidylinositol (GPI)-anchored cell membrane glycoprotein of the UPAR/Ly6 family.  A variety of GPI-linked surface glycoproteins are composed of one or more copies of a conserved domain of ~100 amino-acid residues termed UPAR/Ly6 motif that was originally found in UPAR (Urokinase plasminogen activator surface receptor) and Ly6/CD59.  PSCA contains one UPAR/Ly6 domain in the extracellular domain (ECD). Mature human PSCA (amino acids 21-95) shares ~65% amino acid sequence identity with rodent PSCA.  PSCA may be involved in the regulation of cell proliferation with a cell-proliferation inhibition activity in vitro.  PSCA may be up- or down-regulated in cancer and may promote or suppress the tumor, depending on location. In addition to being highly expressed in the prostate, PSCA is also expressed in the bladder, placenta, colon, kidney, and stomach. PSCA is up-regulated in a large proportion of prostate cancers and is also detected in cancers of the bladder and pancreas. The PSCA gene includes a polymorphism that results in an upstream start codon in some individuals; this polymorphism is thought to be associated with a risk for certain gastric and bladder cancers.

PSCA (prostate stem cell antigen) is a glycosylphosphatidylinositol (GPI)-anchored cell membrane glycoprotein of the UPAR/Ly6 family.  A variety of GPI-linked surface glycoproteins are composed of one or more copies of a conserved domain of ~100 amino-acid residues termed UPAR/Ly6 motif that was originally found in UPAR (Urokinase plasminogen activator surface receptor) and Ly6/CD59.  PSCA contains one UPAR/Ly6 domain in the extracellular domain (ECD). Mature human PSCA (amino acids 21-95) shares ~65% amino acid sequence identity with rodent PSCA.  PSCA may be involved in the regulation of cell proliferation with a cell-proliferation inhibition activity in vitro.  PSCA may be up- or down-regulated in cancer and may promote or suppress the tumor, depending on location. In addition to being highly expressed in the prostate, PSCA is also expressed in the bladder, placenta, colon, kidney, and stomach. PSCA is up-regulated in a large proportion of prostate cancers and is also detected in cancers of the bladder and pancreas. The PSCA gene includes a polymorphism that results in an upstream start codon in some individuals; this polymorphism is thought to be associated with a risk for certain gastric and bladder cancers.

 

Product Details

 

Gene Symbol: PSCA; UNQ206/PRO232

 

NCBI Gene ID: 8000

 

Uniprot Entry: O43653

 

Construct Details: The recombinant human PSCA-Fc is expressed as a 312-amino acid protein consisting of Leu21 -  Ser95 region of PSCA (UniProt accession #O43653) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.   

  

Source:  Human cells stably expressing PSCA-Fc growing in chemical-defined media with no animal components or antibiotics 

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

LLCYSCKAQV SNEDCLQVEN CTQLGEQCWT ARIRAVGLLT VISKGCSLNC VDDSQDYYVG 

        70         80         90        100        110        120 

KKNITCCDTD LCNASTTENL YFQGSTGTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR 

       130        140        150        160        170        180 

TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN 

       190        200        210        220        230        240 

GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR EEMTKNQVSL TCLVKGFYPS 

       250        260        270        280        290        300 

DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH 

       310 

YTQKSLSLSP GK 

 

M.W.: Calculated molecular mass (kDa): 34.8; Estimated by SDS-PAGE under reducing condition (kDa): 50-55

 

Calculated PI: 6.02

 

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm): 47870

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Not available

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Proc. Natl. Acad. Sci. 95:1735 (1998)

2. Biochem. Biophys. Res. Commun. 275:783 (2000)

3. Oncogene 19:1288 (2000)

4. Nat. Genet. 40:730 (2008)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2506-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.