VEGF, also known as VEGF-A (vascular endothelial growth factor A), VPF (vascular permeability factor) or MVCD1 (microvascular complications of diabetes 1), is the founding member of the VEGF family, including VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF.  VEGFs are secreted polypeptides with a highly conserved receptor-binding cystine-knot structure similar to that of the platelet-derived growth factors (PDGF).  VEGF plays important roles in vascular development and in diseases involving abnormal growth of blood vessels such as tumor-related angiogenesis.  VEGF is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. It induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and increases permeabilization of blood vessels. VEGF is known to bind to the FLT1 (VEGFR1) and KDR (VEGFR2) receptors, heparan sulfate and heparin. Targeting VEGF and its signaling pathway has shown therapeutic benefits in multiple cancer types. The purified recombinant monoclonal antibody binds both human and mouse VEGF with high affinity.  It potently blocks VEGF signaling activity.  It also inhibits HUVEC proliferation and tumor cell growth.

VEGF, also known as VEGF-A (vascular endothelial growth factor A), VPF (vascular permeability factor) or MVCD1 (microvascular complications of diabetes 1), is the founding member of the VEGF family, including VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF.  VEGFs are secreted polypeptides with a highly conserved receptor-binding cystine-knot structure similar to that of the platelet-derived growth factors (PDGF).  VEGF plays important roles in vascular development and in diseases involving abnormal growth of blood vessels such as tumor-related angiogenesis.  VEGF is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. It induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and increases permeabilization of blood vessels. VEGF is known to bind to the FLT1 (VEGFR1) and KDR (VEGFR2) receptors, heparan sulfate and heparin. Targeting VEGF and its signaling pathway has shown therapeutic benefits in multiple cancer types. The purified recombinant monoclonal antibody binds both human and mouse VEGF with high affinity.  It potently blocks VEGF signaling activity.  It also inhibits HUVEC proliferation and tumor cell growth.

 

Product Details

 

Gene Symbol: VEGFA; VEGF-A; VPF; MVCD1

 

NCBI Gene ID: 7422

 

Uniprot Entry: P15692

 

Construct Details: Expressed as the combination of a heavy chain (HC) containing VH from anti-human VEGF mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-human VEGF mAb and CL of human kappa light chain.  Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition (see the gel image above, labeled as DTT "-").

 

Source:  Mamamlian cells stably expressing anti-human VEGF human heavy chain and light chain and growing in chemical-defined media with no animal components or antibiotics

 

M.W.: Calculated molecular mass (kDa): 146 (49 kDa for a single HC, 24 kDa for a single LC); Estimated by SDS-PAGE under reducing condition(kDa): 50 kDa + 25 kDa.

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds human VEGF (SKU#FCL0387, F#CL0388) with high affinity  (K_D < 10 nM) as measured by ELISA.  Inhibits HUVEC proliferation and tumor cell growth. Has the same binding specificity as therapeutic anti-VEGF antibody: Avastin® (bevacizumab).

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Science 246:1306-1309, 1989
2. Science 246:1309-1312, 1989
3. Nature 362: 841-844, 1993
4. Semin Oncol 29:10–14, 2002
5. J Biol Chem 281: 6625–31,2006

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here. MAB2628-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.