The leukocyte adhesion molecule CD226, also known as DNAM1 (DNAX accessory molecule­1) and PTA1 (platelet and T cell activation antigen 1), is a single-pass type I transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily (IgSF). CD226 contains a 236 amino acid (aa) extracellular domain (ECD) with 2 Ig­like C2­set (IgC2) domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins. CD226 is identified as an intercellular adhesion molecule, which is constitutively expressed on the majority of CD4+ and CD8+ T lymphocytes, natural killer (NK) cells, monocytes/macrophages, and a subset of B lymphocytes. CD226 is expressed on multiple lymphoid and myeloid cells and interacts with the poliovirus receptor CD155/PVR and its family member CD112/Nectin­2. Ligation of CD226 promotes the activation of NK cells, CD8+ T cells, and mast cells, dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; but inhibits the forrmation of osteoclasts. CD226 is critical for NK cell- and CD8+ T cell-mediated cytotoxicity.  It also plays an important role in immune surveillance of tumor development and is involved in triggering naive T cell differentiation and proliferation.  Platelet­endothelium interactions mediated by CD226 may contribute to the metastasis of tumor cells to the lung.

The leukocyte adhesion molecule CD226, also known as DNAM1 (DNAX accessory molecule­1) and PTA1 (platelet and T cell activation antigen 1), is a single-pass type I transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily (IgSF). CD226 contains a 236 amino acid (aa) extracellular domain (ECD) with 2 Ig­like C2­set (IgC2) domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins. CD226 is identified as an intercellular adhesion molecule, which is constitutively expressed on the majority of CD4+ and CD8+ T lymphocytes, natural killer (NK) cells, monocytes/macrophages, and a subset of B lymphocytes. CD226 is expressed on multiple lymphoid and myeloid cells and interacts with the poliovirus receptor CD155/PVR and its family member CD112/Nectin­2. Ligation of CD226 promotes the activation of NK cells, CD8+ T cells, and mast cells, dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; but inhibits the forrmation of osteoclasts. CD226 is critical for NK cell- and CD8+ T cell-mediated cytotoxicity.  It also plays an important role in immune surveillance of tumor development and is involved in triggering naive T cell differentiation and proliferation.  Platelet­endothelium interactions mediated by CD226 may contribute to the metastasis of tumor cells to the lung.

 

Product Details

 

Gene Symbol: CD226; PTA1; DNAM1; DNAM-1; TLiSA1

 

NCBI Gene ID: 10666

 

Uniprot Entry: Q15762

 

Construct Details: The recombinant human CD226-Fc fusion is expressed as a 466 amino acid protein consisting of Glu19 - Gly256 region of CD226 (Uniprot Accession #Q15762) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source:  Human cells stably expressing CD226-Fc growing in chemical-defined media with no animal components or antibiotics 

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

EEVLWHTSVP FAENMSLECV YPSMGILTQV EWFKIGTQQD SIAIFSPTHG MVIRKPYAER 

        70         80         90        100        110        120 

VYFLNSTMAS NNMTLFFRNA SEDDVGYYSC SLYTYPQGTW QKVIQVVQSD SFEAAVPSNS 

       130        140        150        160        170        180 

HIVSEPGKNV TLTCQPQMTW PVQAVRWEKI QPRQIDLLTY CNLVHGRNFT SKFPRQIVSN 

       190        200        210        220        230        240 

CSHGRWSVIV IPDVTVSDSG LYRCYLQASA GENETFVMRL TVAEGKTDNQ YTLFVAGGST 

       250        260        270        280        290        300 

GTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG 

       310        320        330        340        350        360 

VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG 

       370        380        390        400        410        420 

QPREPQVYTL PPSREEMTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD 

       430        440        450        460 

GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK 

 

M.W.: Calculated molecular mass (kDa): 52.5; Estimated by SDS-PAGE under reducing condition (kDa): 70-75

 

Calculated PI: 6.34

 

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm): 85550

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Immobilized CD226 interacts with Necl5/PVR/CD155 (SKU#FCL1077) and CD112 in a functional ELISA.

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Immunity 4:573 (1996)

2. J. Immunol. 166:1671 (1998)

3. J. Exp. Med. 198:557 (2003)

4. J. Biol. Chem. 278:36748 (2003)

5. J. Immunol. 175:1558 (2005)

6. Blood 107:1491 (2006)

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2323B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.