GPNMB (transmembrane glycoprotein NMB), also known as Osteoactivin and HGFIN (hematopoietic growth factor-inducible neurokinin 1 ), is a single pass type I transmembrane glycoprotein of the NMB/pMEL­17 family. GPNMB is expressed in many cell types, including osteoclasts, macrophages, fibroblasts, dendritic cells, retinal pigment epithelial cells, melanocytes and tumor cells. It is often associated with the endosomal/lysosomal compartment.  GPNMB is composed of a 465 aa luminal/extracellular domain (ECD), a transmembrane segment and a 53 aa cytoplasmic tail. The ECD contains a heparin­binding motif, multiple glycosylation sites, an RGD motif and an 88 aa PKD domain.  The cytoplasmic tail has an ITAM (Y­x­x­I) and lysosomal targeting (L­L) motif. GPNMB may paly a role in osteoblast maturation, cell adhesion and migration. GPNMB may mediate BMP-2 effects on osteoblast differentiation and function. GPNMB may also participates in bone mineralization, and acts as a negative regulator of inflammation in macrophages. GPNMB expression downregulates the macrophage inflammatory response by inhibiting IL­6 and IL­12 production.  GPNMB is reported to bind to heparan sulfate­proteoglycan and integrins in mice. GPNMB is expressed at high levels in normal and inflammatory liver macrophages implicating a role in acute liver injury. GPNMB may also play a role in triggering renal interstitial fibrosis through activation of matrix metalloproteinase expression and collagen remodeling. GPNMB is expressed in human breast cancers and is capable of promoting breast cancer. 

GPNMB (transmembrane glycoprotein NMB), also known as Osteoactivin and HGFIN (hematopoietic growth factor-inducible neurokinin 1 ), is a single pass type I transmembrane glycoprotein of the NMB/pMEL­17 family. GPNMB is expressed in many cell types, including osteoclasts, macrophages, fibroblasts, dendritic cells, retinal pigment epithelial cells, melanocytes and tumor cells. It is often associated with the endosomal/lysosomal compartment.  GPNMB is composed of a 465 aa luminal/extracellular domain (ECD), a transmembrane segment and a 53 aa cytoplasmic tail. The ECD contains a heparin­binding motif, multiple glycosylation sites, an RGD motif and an 88 aa PKD domain.  The cytoplasmic tail has an ITAM (Y­x­x­I) and lysosomal targeting (L­L) motif. GPNMB may paly a role in osteoblast maturation, cell adhesion and migration. GPNMB may mediate BMP-2 effects on osteoblast differentiation and function. GPNMB may also participates in bone mineralization, and acts as a negative regulator of inflammation in macrophages. GPNMB expression downregulates the macrophage inflammatory response by inhibiting IL­6 and IL­12 production.  GPNMB is reported to bind to heparan sulfate­proteoglycan and integrins in mice. GPNMB is expressed at high levels in normal and inflammatory liver macrophages implicating a role in acute liver injury. GPNMB may also play a role in triggering renal interstitial fibrosis through activation of matrix metalloproteinase expression and collagen remodeling. GPNMB is expressed in human breast cancers and is capable of promoting breast cancer. 

 

Product Details

 

Gene Symbol: GPNMB; NMB; HGFIN; UNQ1725/PRO9925

 

NCBI Gene ID: 10457

 

Uniprot Entry: Q14956

 

Construct Details: The recombinant human GPNMB-Fc fusion is expressed as a 693 amino acid protein consisting of Ala22 - Pro486 region of GPNMB (Uniprot Accession #Q14956) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source:  Human cells stably expressing GPNMB-Fc growing in chemical-defined media with no animal components or antibiotics 

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

AKRFHDVLGN ERPSAYMREH NQLNGWSSDE NDWNEKLYPV WKRGDMRWKN SWKGGRVQAV 

        70         80         90        100        110        120 

LTSDSPALVG SNITFAVNLI FPRCQKEDAN GNIVYEKNCR NEAGLSADPY VYNWTAWSED 

       130        140        150        160        170        180 

SDGENGTGQS HHNVFPDGKP FPHHPGWRRW NFIYVFHTLG QYFQKLGRCS VRVSVNTANV 

       190        200        210        220        230        240 

TLGPQLMEVT VYRRHGRAYV PIAQVKDVYV VTDQIPVFVT MFQKNDRNSS DETFLKDLPI 

       250        260        270        280        290        300 

MFDVLIHDPS HFLNYSTINY KWSFGDNTGL FVSTNHTVNH TYVLNGTFSL NLTVKAAAPG 

       310        320        330        340        350        360 

PCPPPPPPPR PSKPTPSLAT TLKSYDSNTP GPAGDNPLEL SRIPDENCQI NRYGHFQATI 

       370        380        390        400        410        420 

TIVEGILEVN IIQMTDVLMP VPWPESSLID FVVTCQGSIP TEVCTIISDP TCEITQNTVC 

       430        440        450        460        470        480 

SPVDVDEMCL LTVRRTFNGS GTYCVNLTLG DDTSLALTST LISVPSTGTH TCPPCPAPEL 

       490        500        510        520        530        540 

LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE 

       550        560        570        580        590        600 

QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS 

       610        620        630        640        650        660 

REEMTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK 

       670        680        690 

SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGK 

 

M.W.: Calculated molecular mass (kDa): 77.6; Estimated by SDS-PAGE under reducing condition (kDa): 95-100

 

Calculated PI: 6.08

 

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm): 120750

 

Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Immobilized GPNMB-Fc induces pro­MMP­9 secretion in human normal lung fibroblasts (NHLF).

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C for immediate use. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. J. Biol. Chem. 276:8125 (2001)

2. Nat. Genet. 30:81 (2002). 

3. Proc. Natl. Acad. Sci. 102:16013 (2005).

4. J. Hepatol. 42:565 (2005).

5. Clin. Cancer Res. 12:1970 (2006).

6. J. Immunol. 178:6557 (2007)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2301-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.