GPNMB (transmembrane glycoprotein NMB), also known as Osteoactivin and HGFIN (hematopoietic growth factor-inducible neurokinin 1 ), is a single pass type I transmembrane glycoprotein of the NMB/pMEL­17 family. GPNMB is expressed in many cell types, including osteoclasts, macrophages, fibroblasts, dendritic cells, retinal pigment epithelial cells, melanocytes and tumor cells. It is often associated with the endosomal/lysosomal compartment.  GPNMB is composed of a 465 aa luminal/extracellular domain (ECD), a transmembrane segment and a 53 aa cytoplasmic tail. The ECD contains a heparin­binding motif, multiple glycosylation sites, an RGD motif and an 88 aa PKD domain.  The cytoplasmic tail has an ITAM (Y­x­x­I) and lysosomal targeting (L­L) motif. GPNMB may paly a role in osteoblast maturation, cell adhesion and migration. GPNMB may mediate BMP-2 effects on osteoblast differentiation and function. GPNMB may also participates in bone mineralization, and acts as a negative regulator of inflammation in macrophages. GPNMB expression downregulates the macrophage inflammatory response by inhibiting IL­6 and IL­12 production.  GPNMB is reported to bind to heparan sulfate­proteoglycan and integrins in mice. GPNMB is expressed at high levels in normal and inflammatory liver macrophages implicating a role in acute liver injury. GPNMB may also play a role in triggering renal interstitial fibrosis through activation of matrix metalloproteinase expression and collagen remodeling. GPNMB is expressed in human breast cancers and is capable of promoting breast cancer. 

GPNMB (transmembrane glycoprotein NMB), also known as Osteoactivin and HGFIN (hematopoietic growth factor-inducible neurokinin 1 ), is a single pass type I transmembrane glycoprotein of the NMB/pMEL­17 family. GPNMB is expressed in many cell types, including osteoclasts, macrophages, fibroblasts, dendritic cells, retinal pigment epithelial cells, melanocytes and tumor cells. It is often associated with the endosomal/lysosomal compartment.  GPNMB is composed of a 465 aa luminal/extracellular domain (ECD), a transmembrane segment and a 53 aa cytoplasmic tail. The ECD contains a heparin­binding motif, multiple glycosylation sites, an RGD motif and an 88 aa PKD domain.  The cytoplasmic tail has an ITAM (Y­x­x­I) and lysosomal targeting (L­L) motif. GPNMB may paly a role in osteoblast maturation, cell adhesion and migration. GPNMB may mediate BMP-2 effects on osteoblast differentiation and function. GPNMB may also participates in bone mineralization, and acts as a negative regulator of inflammation in macrophages. GPNMB expression downregulates the macrophage inflammatory response by inhibiting IL­6 and IL­12 production.  GPNMB is reported to bind to heparan sulfate­proteoglycan and integrins in mice. GPNMB is expressed at high levels in normal and inflammatory liver macrophages implicating a role in acute liver injury. GPNMB may also play a role in triggering renal interstitial fibrosis through activation of matrix metalloproteinase expression and collagen remodeling. GPNMB is expressed in human breast cancers and is capable of promoting breast cancer. 

 

Product Details

 

Gene Symbol: GPNMB; NMB; HGFIN; UNQ1725/PRO9925

 

NCBI Gene ID: 10457

 

Uniprot Entry: Q14956

 

Construct Details: Full length human GPNMB gene (encoding UniProt #Q14956) is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1719 (bp)

 

Gene Insert Sequence:  

ATGGAATGTCTCTACTATTTCCTGGGATTTCTGCTCCTGGCTGCAAGATTGCCACTTGATGCCGCCAAACGATTTCATGA

TGTGCTGGGCAATGAAAGACCTTCTGCTTACATGAGGGAGCACAATCAATTAAATGGCTGGTCTTCTGATGAAAATGACT

GGAATGAAAAACTCTACCCAGTGTGGAAGCGGGGAGACATGAGGTGGAAAAACTCCTGGAAGGGAGGCCGTGTGCAGGCG

GTCCTGACCAGTGACTCACCAGCCCTCGTGGGCTCAAATATAACATTTGCGGTGAACCTGATATTCCCTAGATGCCAAAA

GGAAGATGCCAATGGCAACATAGTCTATGAGAAGAACTGCAGAAATGAGGCTGGTTTATCTGCTGATCCGTATGTTTACA

ACTGGACAGCATGGTCAGAGGACAGTGACGGGGAAAATGGCACCGGCCAAAGCCATCATAACGTCTTCCCTGATGGGAAA

CCTTTTCCTCACCACCCCGGATGGAGAAGATGGAATTTCATCTACGTCTTCCACACACTTGGTCAGTATTTCCAGAAATT

GGGACGATGTTCAGTGAGAGTTTCTGTGAACACAGCCAATGTGACACTTGGGCCTCAACTCATGGAAGTGACTGTCTACA

GAAGACATGGACGGGCATATGTTCCCATCGCACAAGTGAAAGATGTGTACGTGGTAACAGATCAGATTCCTGTGTTTGTG

ACTATGTTCCAGAAGAACGATCGAAATTCATCCGACGAAACCTTCCTCAAAGATCTCCCCATTATGTTTGATGTCCTGAT

TCATGATCCTAGCCACTTCCTCAATTATTCTACCATTAACTACAAGTGGAGCTTCGGGGATAATACTGGCCTGTTTGTTT

CCACCAATCATACTGTGAATCACACGTATGTGCTCAATGGAACCTTCAGCCTTAACCTCACTGTGAAAGCTGCAGCACCA

GGACCTTGTCCGCCACCGCCACCACCACCCAGACCTTCAAAACCCACCCCTTCTTTAGCAACTACTCTAAAATCTTATGA

TTCAAACACCCCAGGACCTGCTGGTGACAACCCCCTGGAGCTGAGTAGGATTCCTGATGAAAACTGCCAGATTAACAGAT

ATGGCCACTTTCAAGCCACCATCACAATTGTAGAGGGAATCTTAGAGGTTAACATCATCCAGATGACAGACGTCCTGATG

CCGGTGCCATGGCCTGAAAGCTCCCTAATAGACTTTGTCGTGACCTGCCAAGGGAGCATTCCCACGGAGGTCTGTACCAT

CATTTCTGACCCCACCTGCGAGATCACCCAGAACACAGTCTGCAGCCCTGTGGATGTGGATGAGATGTGTCTGCTGACTG

TGAGACGAACCTTCAATGGGTCTGGGACGTACTGTGTGAACCTCACCCTGGGGGATGACACAAGCCTGGCTCTCACGAGC

ACCCTGATTTCTGTTCCTGACAGAGACCCAGCCTCGCCTTTAAGGATGGCAAACAGCGCCCTGATCTCCGTTGGCTGCTT

GGCCATATTTGTCACTGTGATCTCCCTCTTGGTGTACAAAAAACACAAGGAATACAACCCAATAGAAAATAGTCCTGGGA

ATGTGGTCAGAAGCAAAGGCCTGAGTGTCTTTCTCAACCGTGCAAAAGCCGTGTTCTTCCCGGGAAACCAGGAAAAGGAT

CCGCTACTCAAAAACCAAGAATTTAAAGGAGTTTCTTAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. J. Biol. Chem. 276:8125 (2001)

2. Nat. Genet. 30:81 (2002). 

3. Proc. Natl. Acad. Sci. 102:16013 (2005).

4. J. Hepatol. 42:565 (2005).

5. Clin. Cancer Res. 12:1970 (2006).

6. J. Immunol. 178:6557 (2007)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTP0933-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.