NCR1, also known as NKp46 and CD335, is a single-pass type I transmembrane glycoprotein that contains two extracellular Immunoglobulin (Ig)-like domains and a short cytoplasmic tail.  CD335 together with NCR2/NKp44/CD336 and NCR3/NKp30/CD337 are activating receptors collectively termed the natural cytotoxicity receptors (NCRs). All these receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and are primarily responsible for triggering NK cell-mediated lytic activities of human tumor cells. CD335 and its co-receptor CD59 together activate cytotoxicity of human NK-cells by the association with ITAM­ bearing signal adapter proteins, including CD3ζ and FcεR1γ, which are able to form disulfide­linked homodimers and heterodimers. Blocking any of these three NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously. CD335 is also implicated in NK cell-mediated lysis of pathogen-infected cell lines and mononuclear phagocytes infected with an intracellular bacterium. CD335 recognizes virus­infected cells through its capacity to bind to viral hemagglutinins.  CD335 is involved in NK-cell cell death by apoptosis. 

NCR1, also known as NKp46 and CD335, is a single-pass type I transmembrane glycoprotein that contains two extracellular Immunoglobulin (Ig)-like domains and a short cytoplasmic tail.  CD335 together with NCR2/NKp44/CD336 and NCR3/NKp30/CD337 are activating receptors collectively termed the natural cytotoxicity receptors (NCRs). All these receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and are primarily responsible for triggering NK cell-mediated lytic activities of human tumor cells. CD335 and its co-receptor CD59 together activate cytotoxicity of human NK-cells by the association with ITAM­ bearing signal adapter proteins, including CD3ζ and FcεR1γ, which are able to form disulfide­linked homodimers and heterodimers. Blocking any of these three NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously. CD335 is also implicated in NK cell-mediated lysis of pathogen-infected cell lines and mononuclear phagocytes infected with an intracellular bacterium. CD335 recognizes virus­infected cells through its capacity to bind to viral hemagglutinins.  CD335 is involved in NK-cell cell death by apoptosis. 

 

Product Details

 

Gene Symbol: NCR1; LY94; CD335; NKP46; NK-p46; hNKp46; NCTR1

 

NCBI Gene ID: 9437

 

Uniprot Entry: O76036

 

Construct Details: The recombinant human NCR1/CD335-Fc fusion is expressed as a 470 amino acid protein consisting of Gln22- Gln254 region of NCR1/CD335 (Uniprot Accession #O76036) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source:  Human cells stably expressing NCR1/CD335-Fc growing in chemical-defined media with no animal components or antibiotics 

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

QQQTLPKPFI WAEPHFMVPK EKQVTICCQG NYGAVEYQLH FEGSLFAVDR PKPPERINKV 

        70         80         90        100        110        120 

KFYIPDMNSR MAGQYSCIYR VGELWSEPSN LLDLVVTEMY DTPTLSVHPG PEVISGEKVT 

       130        140        150        160        170        180 

FYCRLDTATS MFLLLKEGRS SHVQRGYGKV QAEFPLGPVT TAHRGTYRCF GSYNNHAWSF  

       190        200        210        220        230        240 

PSEPVKLLVT GDIENTSLAP EDPTFPDTWG TYLLTTETGL QKDHALWDHT AQSTTENLYF 

       250        260        270        280        290        300 

QGSTGTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW 

       310        320        330        340        350        360 

YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS 

       370        380        390        400        410        420 

KAKGQPREPQ VYTLPPSREE MTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV 

       430        440        450        460        470 

LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK 

 

M.W.: Calculated molecular mass (kDa): 53; Estimated by SDS-PAGE under reducing condition (kDa): ~65

 

Calculated PI: 6.13

 

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm): 81450

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to human vimentin and hemagglutinins.  Blocks partially NK-cell mediated cytotoxicity.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C for immediate use. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. J. Exp. Med. 188:953 (1997) 

2. Nature 409:1055 (2001)

3.Blood 103:664 (2004)

4. J. Immunol. 172:1455 (2004)

5. Blood. 105: 251 (2005)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2196B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.