NCR1, also known as NKp46 and CD335, is a single-pass type I transmembrane glycoprotein that contains two extracellular Immunoglobulin (Ig)-like domains and a short cytoplasmic tail.  CD335 together with NCR2/NKp44/CD336 and NCR3/NKp30/CD337 are activating receptors collectively termed the natural cytotoxicity receptors (NCRs). All these receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and are primarily responsible for triggering NK cell-mediated lytic activities of human tumor cells. CD335 and its co-receptor CD59 together activate cytotoxicity of human NK-cells by the association with ITAM­ bearing signal adapter proteins, including CD3ζ and FcεR1γ, which are able to form disulfide­linked homodimers and heterodimers. Blocking any of these three NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously. CD335 is also implicated in NK cell-mediated lysis of pathogen-infected cell lines and mononuclear phagocytes infected with an intracellular bacterium. CD335 recognizes virus­infected cells through its capacity to bind to viral hemagglutinins.  CD335 is involved in NK-cell cell death by apoptosis. 

NCR1, also known as NKp46 and CD335, is a single-pass type I transmembrane glycoprotein that contains two extracellular Immunoglobulin (Ig)-like domains and a short cytoplasmic tail.  CD335 together with NCR2/NKp44/CD336 and NCR3/NKp30/CD337 are activating receptors collectively termed the natural cytotoxicity receptors (NCRs). All these receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and are primarily responsible for triggering NK cell-mediated lytic activities of human tumor cells. CD335 and its co-receptor CD59 together activate cytotoxicity of human NK-cells by the association with ITAM­ bearing signal adapter proteins, including CD3ζ and FcεR1γ, which are able to form disulfide­linked homodimers and heterodimers. Blocking any of these three NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously. CD335 is also implicated in NK cell-mediated lysis of pathogen-infected cell lines and mononuclear phagocytes infected with an intracellular bacterium. CD335 recognizes virus­infected cells through its capacity to bind to viral hemagglutinins.  CD335 is involved in NK-cell cell death by apoptosis. 

 

Product Details

 

Gene Symbol: NCR1; LY94; CD335; NKP46; NK-p46; hNKp46; NCTR1

 

NCBI Gene ID: 9437

 

Uniprot Entry: O76036

 

Construct Details: Full length human NCR1/CD335/NKp46 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 915 (bp)

 

Gene Insert Sequence:  

ATGTCTTCCACACTCCCTGCCCTGCTCTGCGTCGGGCTGTGTCTGAGTCAGAGGATCAGCGCCCAGCAGC

AGACTCTCCCAAAACCGTTCATCTGGGCCGAGCCCCATTTCATGGTTCCAAAGGAAAAGCAAGTGACCAT

CTGTTGCCAGGGAAATTATGGGGCTGTTGAATACCAGCTGCACTTTGAAGGAAGCCTTTTTGCCGTGGAC

AGACCAAAACCCCCTGAGCGGATTAACAAAGTCCAATTCTACATCCCGGACATGAACTCCCGCATGGCAG

GGCAATACAGCTGCATCTATCGGGTTGGGGAGCTCTGGTCAGAGCCCAGCAACTTGCTGGATCTGGTGGT

AACAGAAATGTATGACACACCCACCCTCTCGGTTCATCCTGGACCCGAAGTGATCTCGGGAGAGAAGGTG

ACCTTCTACTGCCGTCTAGACACTGCAACAAGCATGTTCTTACTGCTCAAGGAGGGAAGATCCAGCCACG

TACAGCGCGGATACGGGAAGGTCCAGGCGGAGTTCCCCCTGGGCCCTGTGACCACAGCCCACAGAGGGAC

ATACCGATGTTTTGGCTCCTATAACAACCATGCCTGGTCTTTCCCCAGTGAGCCAGTGAAGCTCCTGGTC

ACAGGCGACATTGAGAACACCAGCCTTGCACCTGAAGACCCCACCTTTCCTGCAGACACTTGGGGCACCT

ACCTTTTAACCACAGAGACGGGACTCCAGAAAGACCATGCCCTCTGGGATCACACTGCCCAGAATCTCCT

TCGGATGGGCCTGGCCTTTCTAGTCCTGGTGGCTCTAGTGTGGTTCCTGGTTGAAGACTGGCTCAGCAGG

AAGAGGACTAGAGAGCGAGCCAGCAGAGCTTCCACTTGGGAAGGCAGGAGAAGGCTGAACACACAGACTC

TTTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. J. Exp. Med. 188:953 (1997) 

2. Nature 409:1055 (2001)

3.Blood 103:664 (2004)

4. J. Immunol. 172:1455 (2004)

5. Blood. 105: 251 (2005)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTP2153-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.