EPCAM (Epithelial Cellular Adhesion Molecule), also known as CD326, EGP, KS1/4, KSA, gp40, GA733­2, 17­1A, MIC18 and TROP-1, is a single-pass type I transmembrane glycoprotein.  It is composed of a 242 amino acid (aa) extracellular domain (ECD) with 2 EGF-like repeats, a transmembrane domain, and a 26 aa cytoplasmic domain. EPCAM is expressed on most normal epithelial cells and functions as a homotypic calcium-independent cell adhesion molecule. Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers. EPCAM may act as a physical homophilic interaction molecule at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. It also plays a role in embryonic stem cells proliferation and differentiation. EpCAM expression has been found to increase in actively proliferating epithelia tissues and during adult liver regeneration. EpCAM expression is increased in human malignant neoplasias, with most carcinoma expressing EpCAM. Mutations in this gene result in congenital tufting enteropathy. EPCAM is a target for immunotherapy treatment of human carcinomas. 

EPCAM (Epithelial Cellular Adhesion Molecule), also known as CD326, EGP, KS1/4, KSA, gp40, GA733­2, 17­1A, MIC18 and TROP-1, is a single-pass type I transmembrane glycoprotein.  It is composed of a 242 amino acid (aa) extracellular domain (ECD) with 2 EGF-like repeats, a transmembrane domain, and a 26 aa cytoplasmic domain. EPCAM is expressed on most normal epithelial cells and functions as a homotypic calcium-independent cell adhesion molecule. Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers. EPCAM may act as a physical homophilic interaction molecule at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. It also plays a role in embryonic stem cells proliferation and differentiation. EpCAM expression has been found to increase in actively proliferating epithelia tissues and during adult liver regeneration. EpCAM expression is increased in human malignant neoplasias, with most carcinoma expressing EpCAM. Mutations in this gene result in congenital tufting enteropathy. EPCAM is a target for immunotherapy treatment of human carcinomas. 

 

Product Details

 

Gene Symbol: CD326; Ep-CAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1

 

NCBI Gene ID: 4072

 

Uniprot Entry: P16422

 

Construct Details: The recombinant human EPCAM-Fc fusion is expressed as a 470 amino acid protein consisting of Gln24 - Lys265 region of EPCAM (UniProt accession #P16422)  and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted).  

  

Source:  Human cells stably expressing EPCAM-Fc growing in chemical-defined media with no animal components or antibiotics 

 

Deduced Amino Acid Sequence:

 

        10         20         30         40         50         60 

QEECVCENYK LAVNCFVNNN RQCQCTSVGA QNTVICSKLA AKCLVMKAEM NGSKLGRRAK 

        70         80         90        100        110        120 

PEGALQNNDG LYDPDCDESG LFKAKQCNGT SMCWCVNTAG VRRTDKDTEI TCSERVRTYW 

       130        140        150        160        170        180 

IIIELKHKAR EKPYDSKSLR TALQKEITTR YQLDPKFITS ILYENNVITI DLVQNSSQKT 

       190        200        210        220        230        240 

QNDVDIADVA YYFEKDVKGE SLFHSKKMDL TVNGEQLDLD PGQTLIYYVD EKAPEFSMQG 

       250        260        270        280        290        300 

LKSTGTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW 

       310        320        330        340        350        360 

YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS 

       370        380        390        400        410        420 

KAKGQPREPQ VYTLPPSREE MTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV 

       430        440        450        460        470 

LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK 

 

M.W.: Calculated molecular mass 53 kDa; estimated by SDS-PAGE under reducing condition 55-60 kDa probably due to glycosylation. 

 

Calculated PI:  6.56 

 

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm):  62435

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier and preservative free).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds anti-EPCAM monoclonal antibodies, human IgG1 (SKU#MAB2084) and rabbit IgG (SKU#MAB2100).  

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Proc. Natl. Acad. Sci. 87: 2755 (1990) 

2. J. Cell. Biol. 125: 437 (1994)

3. Mol. Cell. Biol. 18:4388 (1998)

4. J. Biol. Chem. 276: 5804 (2001)

5. Cancer. Res. 67: 10831 (2007)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2042-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.