The mannose 6-phophate receptors (MPRs) are single-pass type I transmembrane glycoproteins that target the newly synthesized hydrolases from the Golgi complex and the cell surface to lysosomes. There are two different MPRs, one of ~300kDa termed CI-MPR (cation-independent) and a smaller, dimeric receptor of ~46kDa termed CD-MPR (cation-depnendent). Both MPRss are P-type lectins that bind terminal mannose 6-phosphate (M6P) with similar affinity (CI-MPR = 7 μM, CD-MPR = 8 μM) and contain similar cytoplasmic signals for intracellular trafficking. CD-MPR contain a 157 amino-acid M6P-binding-domain in the extracellular/luminal region. CI-MPR was also identified as the cell surface receptor for insulin-like growth factor 2 (IGF2R/CD222). IGF2R consists of 15 repeats homologous to the M6P-binding domain in CD-MPR. Repeats #3 and #9 are responsible for binding to M6P.  Repeat #11 binds IGF2 while repeat #13 contains a fibronectin type II motif that assists in IGF2 binding. IGF2R regulates many diverse biological processes such as intracellular trafficking, internalization of extracellular factors and modulation of cellular responses. It binds many non­M6P-containing ligands such as retinoic acid, plasminogen­activator receptor and plasminogen, as well as M6P­ containing molecules, including lysosomal enzymes, TGFβ, proliferin, LIF, CD26, herpes simplex glycoprotein D and granzymes.  IGF2R acts as a positive regulator of T-cell coactivation by binding CD26. IGF2R also functions in the activation of TGFβ, and the regulation of IGF2 levels.  Many studies also show that IGF2R is associated with the progression of tumorigenesis. 

The mannose 6-phophate receptors (MPRs) are single-pass type I transmembrane glycoproteins that target the newly synthesized hydrolases from the Golgi complex and the cell surface to lysosomes. There are two different MPRs, one of ~300kDa termed CI-MPR (cation-independent) and a smaller, dimeric receptor of ~46kDa termed CD-MPR (cation-depnendent). Both MPRss are P-type lectins that bind terminal mannose 6-phosphate (M6P) with similar affinity (CI-MPR = 7 μM, CD-MPR = 8 μM) and contain similar cytoplasmic signals for intracellular trafficking. CD-MPR contain a 157 amino-acid M6P-binding-domain in the extracellular/luminal region. CI-MPR was also identified as the cell surface receptor for insulin-like growth factor 2 (IGF2R/CD222). IGF2R consists of 15 repeats homologous to the M6P-binding domain in CD-MPR. Repeats #3 and #9 are responsible for binding to M6P.  Repeat #11 binds IGF2 while repeat #13 contains a fibronectin type II motif that assists in IGF2 binding. IGF2R regulates many diverse biological processes such as intracellular trafficking, internalization of extracellular factors and modulation of cellular responses. It binds many non­M6P-containing ligands such as retinoic acid, plasminogen­activator receptor and plasminogen, as well as M6P­ containing molecules, including lysosomal enzymes, TGFβ, proliferin, LIF, CD26, herpes simplex glycoprotein D and granzymes.  IGF2R acts as a positive regulator of T-cell coactivation by binding CD26. IGF2R also functions in the activation of TGFβ, and the regulation of IGF2 levels.  Many studies also show that IGF2R is associated with the progression of tumorigenesis. 

 

Product Details

 

Gene Symbol: IGF2R; MPR1; MPRI; CD222; CIMPR; M6P-R

 

NCBI Gene ID: 3482

 

Uniprot Entry: P11717

 

Construct Details: The recombinant human IGF2R-Fc is expressed as a 714 amino acid protein consisting of Met1508 - Leu1992 region of IGF2R (UniProt #P11717, repeat #11-13) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition.

 

Source:  Human cells stably expressing human IGF2R-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

MKSNEHDDCQ VTNPSTGHLF DLSSLSGRAG FTAAYSEKGL VYMSICGENE NCPPGVGACF 

        70         80         90        100        110        120 

GQTRISVGKA NKRLRYVDQV LQLVYKDGSP CPSKSGLSYK SVISFVCRPE AGPTNRPMLI  

       130        140        150        160        170        180 

SLDKQTCTLF FSWHTPLACE QATECSVRNG SSIVDLSPLI HRTGGYEAYD ESEDDASDTN 

       190        200        210        220        230        240 

PDFYINICQP LNPMHGVPCP AGAAVCKVPI DGPPIDIGRV AGPPILNPIA NEIYLNFESS 

       250        260        270        280        290        300 

TPCLADKHFN YTSLIAFHCK RGVSMGTPKL LRTSECDFVF EWETPVVCPD EVRMDGCTLT 

       310        320        330        340        350        360 

DEQLLYSFNL SSLSTSTFKV TRDSRTYSVG VCTFAVGPEQ GGCKDGGVCL LSGTKGASFG 

       370        380        390        400        410        420 

RLQSMKLDYR HQDEAVVLSY VNGDRCPPET DDGVPCVFPF IFNGKSYEEC IIESRAKLWC 

       430        440        450        460        470        480 

STTADYDRDH EWGFCRHSNS YRTSSIIFKC DEDEDIGRPQ VFSEVRGCDV TFEWKTKVVC 

       490        500        510        520        530        540 

PPKKLRTDKT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV 

       550        560        570        580        590        600 

KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV SNKALPAPIE 

       610        620        630        640        650        660 

KTISKAKGQP REPQVYTLPP SREEMTKNQV SLTCLVKGFY PSDIAVEWES NGQPENNYKT 

       670        680        690        700        710 

TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF SCSVMHEALH NHYTQKSLSL SPGK 

  

M.W.:  Calculated molecular mass 79.2 kDa; estimated by SDS-PAGE under reducing condition 85-90 kDa probably due to glycosylation

 

Calculated PI: 5.72

 

Calculated Extinction Coefficients (M-1 cm-1, at 280nm): 90365

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier free & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to its ligand IGF2 and inhibits IGF2-mediated signaling activity.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Proc. Natl. Acad. Sci. 82: 4428 (1985).

2. J. Biol. Chem. 260: 12008 (1985).

3. Proc. Natl. Acad. Sci. 84:5575 (1987). 

4. Nature 329:301 (1987). 

5. J. Biol. Chem. 263:2553 (1988).

 

 

 

 

 

 FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL0031B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.