The TGFβ (transforming growth factor β) superfamily proteins are pleiotropic cytokines that regulate a diverse range of cellular processes, including proliferation, differentiation, migration, adhesion and death.  The TGFβ superfamily elicits 4 signaling pathways: TGFβ, Bone Morphogenic Protein (BMP), Activin, and Nodal.  Each pathway signals through a heteromeric receptor complex composed of at least two type-I and two type-II transmembrane receptors containing cytoplasmic serine/threonine kinase domains. These receptors are distinguished by the presence of a glycine/serine-rich juxta-membrane domain found only in the type I receptors. Either type receptor may initially bind ligand, followed by the recruitment of an alternate-type receptor counterpart to form a signal-activating complex. The type I receptors are also referred to as ALKs (Activin receptor-like kinases). ACVR1B (Activin receptor type-1B), also known as ALK4, SKR2, ActR-IB, and ACVRLK4, is a type I receptor in the TGFβ receptor (TGFBR) family.  ACVR1B acts as the main type I receptor to transduce signals of activin and activin-like ligands. Activin binds either ACVR2A or ACVR2B type II receptor and then forms a complex with ACVR1B to activate AVCR1B through phosphorylation. The activated ACVR1B in turn initiates downstream events by phosphorylation of its effectors to regulate gene expression and affect cellular phenotype. Activin signaling can be modulated by the direct interaction of ACVR1B with cripto or inhibin binding protein.  ACVR1B plays an important role in embryonic cell fate and axis determination, cell proliferation and apoptosis, as well as tumorigenesis.  

The TGFβ (transforming growth factor β) superfamily proteins are pleiotropic cytokines that regulate a diverse range of cellular processes, including proliferation, differentiation, migration, adhesion and death.  The TGFβ superfamily elicits 4 signaling pathways: TGFβ, Bone Morphogenic Protein (BMP), Activin, and Nodal.  Each pathway signals through a heteromeric receptor complex composed of at least two type-I and two type-II transmembrane receptors containing cytoplasmic serine/threonine kinase domains. These receptors are distinguished by the presence of a glycine/serine-rich juxta-membrane domain found only in the type I receptors. Either type receptor may initially bind ligand, followed by the recruitment of an alternate-type receptor counterpart to form a signal-activating complex. The type I receptors are also referred to as ALKs (Activin receptor-like kinases). ACVR1B (Activin receptor type-1B), also known as ALK4, SKR2, ActR-IB, and ACVRLK4, is a type I receptor in the TGFβ receptor (TGFBR) family.  ACVR1B acts as the main type I receptor to transduce signals of activin and activin-like ligands. Activin binds either ACVR2A or ACVR2B type II receptor and then forms a complex with ACVR1B to activate AVCR1B through phosphorylation. The activated ACVR1B in turn initiates downstream events by phosphorylation of its effectors to regulate gene expression and affect cellular phenotype. Activin signaling can be modulated by the direct interaction of ACVR1B with cripto or inhibin binding protein.  ACVR1B plays an important role in embryonic cell fate and axis determination, cell proliferation and apoptosis, as well as tumorigenesis.  

 

Product Details

 

Gene Symbol: ACVR1B; ALK4; ALK-4; SKR2; ACTRIB; ActR-IB; ACVRLK4; ACVRIB, ACVR-IB

 

NCBI Gene ID: 91

 

Uniprot Entry: P36896

 

Construct Details: The recombinant human ACVR1B-Fc fusion protein is expressed as a 331 amino acid protein consisting of Ser24 - Glu126 region of ACVR1B (UniProt accession #P36896) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition (see the gel image above, labeled as "DTT: -")

 

Source: Human cells stably expressing human ACVR1B-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence:

SGPRGVQALLCACTSCLQANYTCETDGACMVSIFNLDGMEHHVRTCIPK

VELVPAGKPFYCLSSEDLRNTHCCYTDYCNRIDLRVPSGHLKEPEHPSM

WGPVESTGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVV

VDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQD

WLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKN

QVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKL

TVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK

 

M.W.: Calculated molecular mass (kDa): 37.0; Estimated by SDS-PAGE under reducing condition (kDa): ~45

 

Calculated PI: 6.31

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  47870

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Immobilized ACVR1B binds human Cripto in a functional ELISA.  Blocks Cripto-mediated signaling activity.

 

 

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Oncogene 8:2879 (1993).   

2. Proc. Natl. Acad. Sci. 91:7957 (1994).   

3. Mol. Cell. Biol. 16:1066 (1996).   

4. Genes Dev. 12:844 (1998).  

4. Nature 404:411 (2000).  

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL0140B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.