B7­H4 (B7 homolog 4), also known as B7X, B7H4, B7S1, and VTCN1, is a single pass type I transmembrane glycoprotein that belongs to the B7 family of the Immunoglobulin (Ig) superfamily. Like other B7 family members, B7-H4 contains one Ig V-like and one Ig C-like domain in the extracellular region. Among the family members, they share about 20-25% amino acid identity. B7­H4 is expressed on the surface of activated lymphocytes, macrophages, monocytes, dendritic cells, epithelial cells, and bone marrow­derived mesenchymal stem cells. It can be up-regulated by IL6  and IL10 and inhibited by CSF2 / GM-CSF and IL4 / interleukin-4 on antigen-presenting cells. B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, B7-H4 plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. B7H4 is also involved in promoting epithelial cell transformation. B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis.  A soluble form of B7­H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status. 

B7­H4 (B7 homolog 4), also known as B7X, B7H4, B7S1, and VTCN1, is a single pass type I transmembrane glycoprotein that belongs to the B7 family of the Immunoglobulin (Ig) superfamily. Like other B7 family members, B7-H4 contains one Ig V-like and one Ig C-like domain in the extracellular region. Among the family members, they share about 20-25% amino acid identity. B7­H4 is expressed on the surface of activated lymphocytes, macrophages, monocytes, dendritic cells, epithelial cells, and bone marrow­derived mesenchymal stem cells. It can be up-regulated by IL6  and IL10 and inhibited by CSF2 / GM-CSF and IL4 / interleukin-4 on antigen-presenting cells. B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, B7-H4 plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. B7H4 is also involved in promoting epithelial cell transformation. B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis.  A soluble form of B7­H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status. 

 

Product Details

 

Gene Symbol: B7-H4; VTCN1; B7H4; B7x; B7S1; B7h.5; PRO1291; UNQ659/PRO1291

 

NCBI Gene ID: 79679

 

Uniprot Entry: Q7Z7D3

 

Construct Details: The recombinant human B7-H4-Fc fusion protein is expressed as a 472 amino acid protein consisting of Leu25 - Ser259 region of B7-H4 (UniProt accession #Q7Z7D3) and a C-terminal His-tag. It contains 3 potential sites for N-linked glycosylation.

 

Source:  Human cells stably expressing B7-H4-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence:

LIIGFGISGRHSITVTTVASAGNIGEDGILSCTFEPDIKLSDIVIQWLKEGVLGLVHEFKEGKDELSEQDEMF

RGRTAVFADQVIVGNASLRLKNVQLTDAGTYKCYIITSKGKGNANLEYKTGAFSMPEVNVDYNASSETLRCEA

PRWFPQPTVVWASQVDQGANFSEVSNTSFELNSENVTMKVVSVLYNVTINNTYSCMIENDIAKATGDIKVTES

EIKRRSHLQLLNSKASTTENLYFQGSTGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVS

HEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAK

GQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVD

KSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK

 

M.W.:  Calculated molecular mass 52.4 kDa; estimated by SDS-PAGE under reducing condition 65-75 kDa probably due to glycosylation

 

Calculated PI: 5.78

 

Calculated Extinction Coefficients (M-1 cm-1, at 280nm): 62965

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Inhibits anti­-CD3 antibody induced IL­2 secretion in human T cells with an ED₅₀ of 0.5 - 2.5 μg/ml 

  

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Proc. Natl. Acad. Sci. 100:10388 (2003).  

2. Immunity 18:863 (2003).  

3. Immunity 18:849 (2003).  

4. Clin. Cancer Res. 11:1842 (2005).

5. J. Exp. Med. 203:871 (2006).  

 

 

 

 FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL0745B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.