TACI (transmembrane activator and CAML ­interactor), also known as CD267, is a single-pass, type III transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF13B). The TNFRSF members have crucial roles in both innate and adaptive immunity as well as in apoptosis. The extracellular region of TACI contains two TNFR-cysteine rich repeats that mediate interactions with its TNF family ligands, including APRIL/TNFSF13 and BAFF/TNFSF13B. TACI binds both ligands with similar high affinity.  Within the TNFRSF, it shares the highest homology with BCMA/TNFRSF17. TACI and BCMA can bind both APRIL and BAFF. TACI is expressed on the cell surface of B cells and activated T cells, but not on resting T cells. TACI may play an important role in B cell development, function and regulation. TACI interacts with calcium-modulator and cyclophilin ligand (CAML). TACI mediates calcineurin-dependent activation of NF-AT, NF-κB and AP-1.  TACI is also involved in the stimulation of B- and T-cell function and the regulation of humoral immunity.  Mutations in the TACI gene are associated with common variable immunodeficiency 2 (CVID2) and immunoglobulin A deficiency 2 (IGAD2) in humans.

TACI (transmembrane activator and CAML ­interactor), also known as CD267, is a single-pass, type III transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF13B). The TNFRSF members have crucial roles in both innate and adaptive immunity as well as in apoptosis. The extracellular region of TACI contains two TNFR-cysteine rich repeats that mediate interactions with its TNF family ligands, including APRIL/TNFSF13 and BAFF/TNFSF13B. TACI binds both ligands with similar high affinity.  Within the TNFRSF, it shares the highest homology with BCMA/TNFRSF17. TACI and BCMA can bind both APRIL and BAFF. TACI is expressed on the cell surface of B cells and activated T cells, but not on resting T cells. TACI may play an important role in B cell development, function and regulation. TACI interacts with calcium-modulator and cyclophilin ligand (CAML). TACI mediates calcineurin-dependent activation of NF-AT, NF-κB and AP-1.  TACI is also involved in the stimulation of B- and T-cell function and the regulation of humoral immunity.  Mutations in the TACI gene are associated with common variable immunodeficiency 2 (CVID2) and immunoglobulin A deficiency 2 (IGAD2) in humans.

  

Product Details

 

Gene Symbol: TACI; CD267; CVID; RYZN; CVID2; TNFRSF13B

 

NCBI Gene ID: 23495

 

Uniprot Entry: O14836

 

Construct Details: Full length human TACI/CD267 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Sequence:  

ATGAGTGGCCTGGGCCGGAGCAGGCGAGGTGGCCGGAGCCGTGTGGACCAGGAGGAGCGCTTTCCACAGGGCCTGTGGAC

GGGGGTGGCTATGAGATCCTGCCCCGAAGAGCAGTACTGGGATCCTCTGCTGGGTACCTGCATGTCCTGCAAAACCATTT

GCAACCATCAGAGCCAGCGCACCTGTGCAGCCTTCTGCAGGTCACTCAGCTGCCGCAAGGAGCAAGGCAAGTTCTATGAC

CATCTCCTGAGGGACTGCATCAGCTGTGCCTCCATCTGTGGACAGCACCCTAAGCAATGTGCATACTTCTGTGAGAACAA

GCTCAGGAGCCCAGTGAACCTTCCACCAGAGCTCAGGAGACAGCGGAGTGGAGAAGTTGAAAACAATTCAGACAACTCGG

GAAGGTACCAAGGATTGGAGCACAGAGGCTCAGAAGCAAGTCCAGCTCTCCCGGGGCTGAAGCTGAGTGCAGATCAGGTG

GCCCTGGTCTACAGCACGCTGGGGCTCTGCCTGTGTGCCGTCCTCTGCTGCTTCCTGGTGGCGGTGGCCTGCTTCCTCAA

GAAGAGGGGGGATCCCTGCTCCTGCCAGCCCCGCTCAAGGCCCCGTCAAAGTCCGGCCAAGTCTTCCCAGGATCACGCGA

TGGAAGCCGGCAGCCCTGTGAGCACATCCCCCGAGCCAGTGGAGACCTGCAGCTTCTGCTTCCCTGAGTGCAGGGCGCCC

ACGCAGGAGAGCGCAGTCACGCCTGGGACCCCCGACCCCACTTGTGCTGGAAGGTGGGGGTGCCACACCAGGACCACAGT

CCTGCAGCCTTGCCCACACATCCCAGACAGTGGCCTTGGCATTGTGTGTGTGCCTGCCCAGGAGGGGGGCCCAGGTGCAT

AA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Science, 278:138 (1997). 

2. J. Exp. Med. 192:137 (2000).

3. Nature 404:995 (2000). 

4. Nature Immunol.1:37 (2000).

5. Nat Genet. 37: 820 (2005)

 

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTP0225-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.