OPG (Osteoprotegerin), also known as OCIF (osteoclastogenesis inhibitory factor), is a secreted 55 - 60 kDa protein of the TNF-receptor superfamily (TNFRSF11B).  Mature OPG is a 380 amino acid soluble protein consisting of 4 TNFR cysteine-rich repeats, 2 death domains (DD) and 1 heparin-binding region. The cysteine-rich repeats are essential for ligand interaction, while a cysteine at the C-terminus mediates homodimerization.  OPG is widely expressed and constitutively released by mesenchymal stem cells, fibroblasts and endothelial cells. OPG is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. OPG specifically binds to its ligand, OPG ligand or RANKL (receptor activator of NF-κB ligand), both of which are key extracellular regulators of osteoclast development. OPG and RANKL may also play a role in lymph-node organogenesis and vascular calcification. OPG regulates bone density through inhibiting the activation of osteoclasts and promoting osteoclast apoptosis.  Bone homeostasis may depend on the local RANKL to OPG ratio. OPG also act as a decoy receptor for TRAIL (TNF-related apoptosis-inducing ligand). TRAIL binding blocks the inhibition of osteoclastogenesis. In addition TRAIL decreases the release of OPG from cells that express it, while OPG inhibits TRAIL-induced apoptosis. Binding of OPG by syndecan-1 heparin sulfates on multiple myeloma cells also results in OPG internalization and degradation, contributing to bone loss. OPG deficiency can cause juvenile Paget’s disease (JPD) in humans, and insufficient OPG can produce osteoporosis and vascular calcification in animals and humans.

OPG (Osteoprotegerin), also known as OCIF (osteoclastogenesis inhibitory factor), is a secreted 55 - 60 kDa protein of the TNF-receptor superfamily (TNFRSF11B).  Mature OPG is a 380 amino acid soluble protein consisting of 4 TNFR cysteine-rich repeats, 2 death domains (DD) and 1 heparin-binding region. The cysteine-rich repeats are essential for ligand interaction, while a cysteine at the C-terminus mediates homodimerization.  OPG is widely expressed and constitutively released by mesenchymal stem cells, fibroblasts and endothelial cells. OPG is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. OPG specifically binds to its ligand, OPG ligand or RANKL (receptor activator of NF-κB ligand), both of which are key extracellular regulators of osteoclast development. OPG and RANKL may also play a role in lymph-node organogenesis and vascular calcification. OPG regulates bone density through inhibiting the activation of osteoclasts and promoting osteoclast apoptosis.  Bone homeostasis may depend on the local RANKL to OPG ratio. OPG also act as a decoy receptor for TRAIL (TNF-related apoptosis-inducing ligand). TRAIL binding blocks the inhibition of osteoclastogenesis. In addition TRAIL decreases the release of OPG from cells that express it, while OPG inhibits TRAIL-induced apoptosis. Binding of OPG by syndecan-1 heparin sulfates on multiple myeloma cells also results in OPG internalization and degradation, contributing to bone loss. OPG deficiency can cause juvenile Paget’s disease (JPD) in humans, and insufficient OPG can produce osteoporosis and vascular calcification in animals and humans.

  

Product Details

 

Gene Symbol: OPG; TR1; OCIF; TNFRSF11B

  

NCBI Gene ID: 4982

 

Uniprot Entry: O00300

 

Construct Details: The recombinant human OPG-Fc fusion is expressed as a 608 amino acid protein consisting of Glu22 - Leu401 region of OPG (UniProt accession #O00300) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted above).  

 

Source: Human cells stably expressing human OPG-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

ETFPPKYLHY DEETSHQLLC DKCPPGTYLK QHCTAKWKTV CAPCPDHYYT DSWHTSDECL 

        70         80         90        100        110        120 

YCSPVCKELQ YVKQECNRTH NRVCECKEGR YLEIEFCLKH RSCPPGFGVV QAGTPERNTV 

       130        140        150        160        170        180 

CKRCPDGFFS NETSSKAPCR KHTNCSVFGL LLTQKGNATH DNICSGNSES TQKCGIDVTL 

       190        200        210        220        230        240 

CEEAFFRFAV PTKFTPNWLS VLVDNLPGTK VNAESVERIK RQHSSQEQTF QLLKLWKHQN 

       250        260        270        280        290        300 

KDQDIVKKII QDIDLCENSV QRHIGHANLT FEQLRSLMES LPGKKVGAED IEKTIKACKP 

       310        320        330        340        350        360 

SDQILKLLSL WRIKNGDQDT LKGLMHALKH SKTYHFPKTV TQSLKKTIRF LHSFTMYKLY 

       370        380        390        400        410        420 

QKLFLEMIGN QVQSVKISCL STGTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV 

       430        440        450        460        470        480 

TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY  

       490        500        510        520        530        540 

KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSREEMT KNQVSLTCLV KGFYPSDIAV 

       550        560        570        580        590        600 

EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK 

 

SLSLSPGK

 

M.W.: Calculated molecular mass (kDa): 69.2; Estimated by SDS-PAGE under reducing condition (kDa): 75-85

 

Calculated PI: 8.55

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 81050

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to its ligands TRAIL and RANKL.  Inhibits TRAIL- and RANKL-mediated signaling activity including NF-kB activation and TRAIL­induced cytotoxicity in L-929 mouse fibroblast cells.

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

1. Cell 89:309 (1997).

2. Proc. Natl. Acad. Sci. 95:3597 (1998). 

3. J. Biol. Chem. 273:5117 (1998). 

4. N. Engl. J. Med. 347:175 (2002). 

5. Bone 39:706 (2006). 

6. J. Atherosclerosis 204:321 (2009).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL0588-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.